Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum

Peters-Wendisch P, Schiel B, Wendisch VF, Katsoulidis E, Mockel B, Sahm H, Eikmanns BJ (2001)
Journal of Molecular Microbiology and Biotechnology 3(2): 295-300.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ; ;
Abstract / Bemerkung
Corynebacterium glutamicum possesses both phosphoenolpyruvate carboxylase (PEPCx) and pyruvate carboxylase (PCx) as anaplerotic enzymes for growth on carbohydrates. To analyze the significance of PCx for the amino acid production by this organism, the wild-type pyc gene, encoding PCx, was used for the construction of defined pyc-inactive and pyc-overexpressing strains and the glutamate, lysine and threonine production capabilities of these recombinant strains of C. glutamicum were tested in comparison to the respective host strains. No PCx activity was observed in the pyc-inactive mutants whereas the pyc-overexpressing strains showed eight-to elevenfold higher specific PCx activity when compared to the host strains. In a detergent-dependent glutamate production assay, the pyc-overexpressing strain showed more than sevenfold higher, the PCx-deficient strain about twofold lower glutamate production than the wild-type, Overexpression of the pyc gene and thus increasing the PCx activity in a lysine-producing strain of C. glutamicum resulted in approximately 50% higher lysine accumulation in the culture supernatant whereas inactivation of the pyc gene led to a decrease by 60%, In a threonine-producing strain of C. glutamicum, the overexpression of the pyc gene led to an only 10 to 20% increase in threonine production, however, to a more than 150% increase in the production of the threonine precursor homoserine. These results identify the anaplerotic PCx reaction as a major bottleneck for amino acid production by C. glutamicum and show that the enzyme is an important target for the molecular breeding of hyperproducing strains.
Erscheinungsjahr
Zeitschriftentitel
Journal of Molecular Microbiology and Biotechnology
Band
3
Zeitschriftennummer
2
Seite
295-300
ISSN
PUB-ID

Zitieren

Peters-Wendisch P, Schiel B, Wendisch VF, et al. Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. Journal of Molecular Microbiology and Biotechnology. 2001;3(2):295-300.
Peters-Wendisch, P., Schiel, B., Wendisch, V. F., Katsoulidis, E., Mockel, B., Sahm, H., & Eikmanns, B. J. (2001). Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. Journal of Molecular Microbiology and Biotechnology, 3(2), 295-300.
Peters-Wendisch, P., Schiel, B., Wendisch, V. F., Katsoulidis, E., Mockel, B., Sahm, H., and Eikmanns, B. J. (2001). Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. Journal of Molecular Microbiology and Biotechnology 3, 295-300.
Peters-Wendisch, P., et al., 2001. Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. Journal of Molecular Microbiology and Biotechnology, 3(2), p 295-300.
P. Peters-Wendisch, et al., “Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum”, Journal of Molecular Microbiology and Biotechnology, vol. 3, 2001, pp. 295-300.
Peters-Wendisch, P., Schiel, B., Wendisch, V.F., Katsoulidis, E., Mockel, B., Sahm, H., Eikmanns, B.J.: Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. Journal of Molecular Microbiology and Biotechnology. 3, 295-300 (2001).
Peters-Wendisch, Petra, Schiel, B., Wendisch, Volker F., Katsoulidis, E., Mockel, B., Sahm, H., and Eikmanns, B. J. “Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum”. Journal of Molecular Microbiology and Biotechnology 3.2 (2001): 295-300.

120 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Emerging Corynebacterium glutamicum systems biology.
Wendisch VF, Bott M, Kalinowski J, Oldiges M, Wiechert W., J Biotechnol 124(1), 2006
PMID: 16406159
Enhanced glutamic acid production by a H+-ATPase-defective mutant of Corynebacterium glutamicum.
Aoki R, Wada M, Takesue N, Tanaka K, Yokota A., Biosci Biotechnol Biochem 69(8), 2005
PMID: 16116273
Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production.
Stansen C, Uy D, Delaunay S, Eggeling L, Goergen JL, Wendisch VF., Appl Environ Microbiol 71(10), 2005
PMID: 16204505
Metabolic engineering of Corynebacterium glutamicum for L-serine production.
Peters-Wendisch P, Stolz M, Etterich H, Kennerknecht N, Sahm H, Eggeling L., Appl Environ Microbiol 71(11), 2005
PMID: 16269752
Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions.
Inui M, Murakami S, Okino S, Kawaguchi H, Vertès AA, Yukawa H., J Mol Microbiol Biotechnol 7(4), 2004
PMID: 15383716
Glutamate as an inhibitor of phosphoenolpyruvate carboxylase activity in Corynebacterium glutamicum.
Delaunay S, Daran-Lapujade P, Engasser JM, Goergen JL., J Ind Microbiol Biotechnol 31(4), 2004
PMID: 15133716
Roles of pyruvate kinase and malic enzyme in Corynebacterium glutamicum for growth on carbon sources requiring gluconeogenesis.
Netzer R, Krause M, Rittmann D, Peters-Wendisch PG, Eggeling L, Wendisch VF, Sahm H., Arch Microbiol 182(5), 2004
PMID: 15375646
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Krämer R, Linke B, McHardy AC, Meyer F, Möckel B, Pfefferle W, Pühler A, Rey DA, Rückert C, Rupp O, Sahm H, Wendisch VF, Wiegräbe I, Tauch A., J Biotechnol 104(1-3), 2003
PMID: 12948626
Acetate metabolism and its regulation in Corynebacterium glutamicum.
Gerstmeir R, Wendisch VF, Schnicke S, Ruan H, Farwick M, Reinscheid D, Eikmanns BJ., J Biotechnol 104(1-3), 2003
PMID: 12948633
The use of enzymes in the chemical industry in Europe.
Schmid A, Hollmann F, Park JB, Bühler B., Curr Opin Biotechnol 13(4), 2002
PMID: 12323359
IGF-I promotes a shift in metabolic flux in vascular smooth muscle cells.
Hall JL, Gibbons GH, Chatham JC., Am J Physiol Endocrinol Metab 283(3), 2002
PMID: 12169439

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 11321586
PubMed | Europe PMC

Suchen in

Google Scholar