NCgl2620 encodes a class II polyphosphate kinase in Corynebactetium glutamicum

Lindner S, Vidaurre D, Willbold S, Schoberth SM, Wendisch VF (2007)
Applied and Environmental Microbiology 73(15): 5026-5033.

Download
No fulltext has been uploaded. References only!
Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Corynebacterium glutamicum is able to accumulate up to 600 mM cytosolic phosphorus in the form of polyphosphate (poly P). Granular poly P (volutin) can make up to 37% of the internal cell volume. This bacterium lacks the classic enzyme of poly P synthesis, class I polyphosphate kinase (PPK1), but it possesses two genes, ppk2A (corresponds to NCg10880) and ppk2B (corresponds to NCg12620), for putative class II (PPK2) PPKs. Deletion of ppk2B decreased PPK activity and cellular poly P content, while overexpression of ppk2B increased both PPK activity and cellular poly P content. Neither deletion nor overexpression of ppk2A changed specific activity of PPK or cellular poly P content significantly. Purified PPK2B of C. glutamicum is active as a bomotetramer and formed poly P with an average chain length of about 125, as determined with P-31 nuclear magnetic resonance. The catalytic efficiency of C. glutamicum PPK2B was higher in the poly P-forming direction than for nucleoside triphosphate formation from poly P. The ppk2B deletion mutant, which accumulated very little poly P and grew as C. glutamicum wild type under phosphate-sufficient conditions, showed a growth defect under phosphate-limiting conditions.
Publishing Year
ISSN
PUB-ID

Cite this

Lindner S, Vidaurre D, Willbold S, Schoberth SM, Wendisch VF. NCgl2620 encodes a class II polyphosphate kinase in Corynebactetium glutamicum. Applied and Environmental Microbiology. 2007;73(15):5026-5033.
Lindner, S., Vidaurre, D., Willbold, S., Schoberth, S. M., & Wendisch, V. F. (2007). NCgl2620 encodes a class II polyphosphate kinase in Corynebactetium glutamicum. Applied and Environmental Microbiology, 73(15), 5026-5033. doi:10.1128/Aem.00600-07
Lindner, S., Vidaurre, D., Willbold, S., Schoberth, S. M., and Wendisch, V. F. (2007). NCgl2620 encodes a class II polyphosphate kinase in Corynebactetium glutamicum. Applied and Environmental Microbiology 73, 5026-5033.
Lindner, S., et al., 2007. NCgl2620 encodes a class II polyphosphate kinase in Corynebactetium glutamicum. Applied and Environmental Microbiology, 73(15), p 5026-5033.
S. Lindner, et al., “NCgl2620 encodes a class II polyphosphate kinase in Corynebactetium glutamicum”, Applied and Environmental Microbiology, vol. 73, 2007, pp. 5026-5033.
Lindner, S., Vidaurre, D., Willbold, S., Schoberth, S.M., Wendisch, V.F.: NCgl2620 encodes a class II polyphosphate kinase in Corynebactetium glutamicum. Applied and Environmental Microbiology. 73, 5026-5033 (2007).
Lindner, Steffen, Vidaurre, D., Willbold, S., Schoberth, S. M., and Wendisch, Volker F. “NCgl2620 encodes a class II polyphosphate kinase in Corynebactetium glutamicum”. Applied and Environmental Microbiology 73.15 (2007): 5026-5033.
This data publication is cited in the following publications:
This publication cites the following data publications:

27 Citations in Europe PMC

Data provided by Europe PubMed Central.

Inorganic polyphosphate: essential for growth and survival.
Rao NN, Gómez-García MR, Kornberg A., Annu Rev Biochem 78(), 2009
PMID: 19344251
Role of polyphosphates in microbial adaptation to extreme environments.
Seufferheld MJ, Alvarez HM, Farias ME., Appl Environ Microbiol 74(19), 2008
PMID: 18708516

44 References

Data provided by Europe PubMed Central.

Inorganic polyphosphate in Vibrio cholerae: genetic, biochemical, and physiologic features.
Ogawa N, Tzeng CM, Fraley CD, Kornberg A., J. Bacteriol. 182(23), 2000
PMID: 11073913

AUTHOR UNKNOWN, 2004
Formation of volutin granules in Corynebacterium glutamicum.
Pallerla SR, Knebel S, Polen T, Klauth P, Hollender J, Wendisch VF, Schoberth SM., FEMS Microbiol. Lett. 243(1), 2005
PMID: 15668011
Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum.
Peters-Wendisch PG, Schiel B, Wendisch VF, Katsoulidis E, Mockel B, Sahm H, Eikmanns BJ., J. Mol. Microbiol. Biotechnol. 3(2), 2001
PMID: 11321586
Nuclear polyphosphate as a possible source of energy during the sporulation of Physarum polycephalum.
Pilatus U, Mayer A, Hildebrandt A., Arch. Biochem. Biophys. 275(1), 1989
PMID: 2817896
Inorganic polyphosphate is required for motility of bacterial pathogens.
Rashid MH, Rao NN, Kornberg A., J. Bacteriol. 182(1), 2000
PMID: 10613886
Polyphosphate kinase is essential for biofilm development, quorum sensing, and virulence of Pseudomonas aeruginosa.
Rashid MH, Rumbaugh K, Passador L, Davies DG, Hamood AN, Iglewski BH, Kornberg A., Proc. Natl. Acad. Sci. U.S.A. 97(17), 2000
PMID: 10931957

AUTHOR UNKNOWN, 1989

AUTHOR UNKNOWN, 1999

AUTHOR UNKNOWN, 1992

AUTHOR UNKNOWN, 1997

AUTHOR UNKNOWN, 1999

AUTHOR UNKNOWN, 2005
A polyphosphate kinase (PPK2) widely conserved in bacteria.
Zhang H, Ishige K, Kornberg A., Proc. Natl. Acad. Sci. U.S.A. 99(26), 2002
PMID: 12486232

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 17545325
PubMed | Europe PMC

Search this title in

Google Scholar