Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum

Lindner S, Knebel S, Pallerla SR, Schoberth SM, Wendisch VF (2010)
Applied Microbiology and Biotechnology 87(2): 703-713.

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The Corynebacterium glutamicum gene cg2091 is encoding a polyphosphate (PolyP)/ATP-dependent glucokinase (PPGK). Previous work demonstrated the association of PPGK to PolyP granules. The deduced amino acid sequence of PPGK shows 45% sequence identity to PolyP/ATP glucomannokinase of Arthrobacter sp. strain KM and 50% sequence identity to PolyP glucokinase of Mycobacterium tuberculosis H37Rv. PPGK from C. glutamicum was purified from recombinant Escherichia coli. PolyP was highly preferred over ATP and other NTPs as substrate and with respect to the tested PolyPs differing in chain length; the protein was most active with PolyP(75). Gel filtration analysis revealed that PolyP supported the formation of homodimers of PPGK and that PPGK was active as a homodimer. A ppgK deletion mutant (Delta ppgK) showed slowed growth in minimal medium with maltose as sole carbon source. Moreover, in minimal medium containing 2 to 4% (w/v) glucose as carbon source, Delta ppgK grew to lower final biomass concentrations than the wild type. Under phosphate starvation conditions, growth of Delta ppgK was reduced, and growth of a ppgK overexpressing strain was increased as compared to wild type and empty vector control, respectively. Thus, under conditions of glucose excess, the presence of PPGK entailed a growth advantage.
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Lindner S, Knebel S, Pallerla SR, Schoberth SM, Wendisch VF. Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum. Applied Microbiology and Biotechnology. 2010;87(2):703-713.
Lindner, S., Knebel, S., Pallerla, S. R., Schoberth, S. M., & Wendisch, V. F. (2010). Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum. Applied Microbiology and Biotechnology, 87(2), 703-713.
Lindner, S., Knebel, S., Pallerla, S. R., Schoberth, S. M., and Wendisch, V. F. (2010). Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum. Applied Microbiology and Biotechnology 87, 703-713.
Lindner, S., et al., 2010. Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum. Applied Microbiology and Biotechnology, 87(2), p 703-713.
S. Lindner, et al., “Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum”, Applied Microbiology and Biotechnology, vol. 87, 2010, pp. 703-713.
Lindner, S., Knebel, S., Pallerla, S.R., Schoberth, S.M., Wendisch, V.F.: Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum. Applied Microbiology and Biotechnology. 87, 703-713 (2010).
Lindner, Steffen, Knebel, S., Pallerla, S. R., Schoberth, S. M., and Wendisch, Volker F. “Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum”. Applied Microbiology and Biotechnology 87.2 (2010): 703-713.
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Biochemistry and regulatory functions of bacterial glucose kinases.
Romero-Rodriguez A, Ruiz-Villafan B, Rocha-Mendoza D, Manzo-Ruiz M, Sanchez S., Arch. Biochem. Biophys. 577-578(), 2015
PMID: 25978980
A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833.
Ikeda M, Noguchi N, Ohshita M, Senoo A, Mitsuhashi S, Takeno S., Appl. Microbiol. Biotechnol. 99(6), 2015
PMID: 25549619
Diversity of phosphorus reserves in microorganisms.
Kulakovskaya TV, Lichko LP, Ryazanova LP., Biochemistry Mosc. 79(13), 2014
PMID: 25749167
All1371 is a polyphosphate-dependent glucokinase in Anabaena sp. PCC 7120.
Klemke F, Beyer G, Sawade L, Saitov A, Korte T, Maldener I, Lockau W, Nurnberg DJ, Volkmer T., Microbiology (Reading, Engl.) 160(Pt 12), 2014
PMID: 25320362
Investigation of ptsG gene in response to xylose utilization in Corynebacterium glutamicum.
Wang C, Cai H, Zhou Z, Zhang K, Chen Z, Chen Y, Wan H, Ouyang P., J. Ind. Microbiol. Biotechnol. 41(8), 2014
PMID: 24859809
Glucose kinases from Streptomyces peucetius var. caesius.
Ruiz-Villafan B, Rodriguez-Sanoja R, Aguilar-Osorio G, Gosset G, Sanchez S., Appl. Microbiol. Biotechnol. 98(13), 2014
PMID: 24687748
Pushing product formation to its limit: metabolic engineering of Corynebacterium glutamicum for L-leucine overproduction.
Vogt M, Haas S, Klaffl S, Polen T, Eggeling L, van Ooyen J, Bott M., Metab. Eng. 22(), 2014
PMID: 24333966
Characterization of polyphosphate glucokinase SCO5059 from Streptomyces coelicolor A3(2).
Koide M, Miyanaga A, Kudo F, Eguchi T., Biosci. Biotechnol. Biochem. 77(11), 2013
PMID: 24200789
Maltose uptake by the novel ABC transport system MusEFGK2I causes increased expression of ptsG in Corynebacterium glutamicum.
Henrich A, Kuhlmann N, Eck AW, Kramer R, Seibold GM., J. Bacteriol. 195(11), 2013
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Accelerated pentose utilization by Corynebacterium glutamicum for accelerated production of lysine, glutamate, ornithine and putrescine.
Meiswinkel TM, Gopinath V, Lindner SN, Nampoothiri KM, Wendisch VF., Microb Biotechnol 6(2), 2013
PMID: 23164409
Metabolic engineering of Corynebacterium glutamicum aimed at alternative carbon sources and new products.
Zahoor A, Lindner SN, Wendisch VF., Comput Struct Biotechnol J 3(), 2012
PMID: 24688664
Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum.
Gopinath V, Meiswinkel TM, Wendisch VF, Nampoothiri KM., Appl. Microbiol. Biotechnol. 92(5), 2011
PMID: 21796382
Phosphotransferase system-independent glucose utilization in corynebacterium glutamicum by inositol permeases and glucokinases.
Lindner SN, Seibold GM, Henrich A, Kramer R, Wendisch VF., Appl. Environ. Microbiol. 77(11), 2011
PMID: 21478323
Link between phosphate starvation and glycogen metabolism in Corynebacterium glutamicum, revealed by metabolomics.
Woo HM, Noack S, Seibold GM, Willbold S, Eikmanns BJ, Bott M., Appl. Environ. Microbiol. 76(20), 2010
PMID: 20802079

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