Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation

Krings E, Krumbach K, Bathe B, Kelle R, Wendisch VF, Sahm H, Eggeling L (2006)
Journal of Bacteriology 188(23): 8054-8061.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ;
Abstract
Although numerous bacteria possess genes annotated iol in their genomes, there have been very few studies on the possibly associated myo-inositol metabolism and its significance for the cell. We found that Corynebacterium glutamicum utilizes myo-inositol as a carbon and energy source, enabling proliferation with a high maximum rate of 0.35 h(-1). Whole-genome DNA microarray analysis revealed that 31 genes respond to myo-inositol utilization, with 21 of them being localized in two clusters of > 14 kb. A set of genomic mutations and functional studies yielded the result that some genes in the two clusters are redundant, and only cluster I is necessary for catabolizing the polyol. There are three genes which encode carriers belonging to the major facilitator superfamily and which exhibit a > 12-fold increased mRNA level on myo-inositol. As revealed by mutant characterizations, one carrier is not involved in myo-inositol uptake whereas the other two are active and can completely replace each other with apparent K(m)s for myo-inositol as a substrate of 0.20 mM and 0.45 mM, respectively. Interestingly, upon utilization of myo-inositol, the L-lysine yield is 0.10 mol/mol, as opposed to 0.30 mol/mol, with glucose as the substrate. This is probably not only due to myo-inositol metabolism alone since a mixture of 187 mM glucose and 17 mM myo-inositol, where the polyol only contributes 8% of the total carbon, reduced the L-lysine yield by 29%. Moreover, genome comparisons with other bacteria highlight the core genes required for growth on myo-inositol, whose metabolism is still weakly defined.
Publishing Year
ISSN
PUB-ID

Cite this

Krings E, Krumbach K, Bathe B, et al. Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. Journal of Bacteriology. 2006;188(23):8054-8061.
Krings, E., Krumbach, K., Bathe, B., Kelle, R., Wendisch, V. F., Sahm, H., & Eggeling, L. (2006). Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. Journal of Bacteriology, 188(23), 8054-8061.
Krings, E., Krumbach, K., Bathe, B., Kelle, R., Wendisch, V. F., Sahm, H., and Eggeling, L. (2006). Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. Journal of Bacteriology 188, 8054-8061.
Krings, E., et al., 2006. Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. Journal of Bacteriology, 188(23), p 8054-8061.
E. Krings, et al., “Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation”, Journal of Bacteriology, vol. 188, 2006, pp. 8054-8061.
Krings, E., Krumbach, K., Bathe, B., Kelle, R., Wendisch, V.F., Sahm, H., Eggeling, L.: Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. Journal of Bacteriology. 188, 8054-8061 (2006).
Krings, E., Krumbach, K., Bathe, B., Kelle, R., Wendisch, Volker F., Sahm, H., and Eggeling, L. “Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation”. Journal of Bacteriology 188.23 (2006): 8054-8061.
This data publication is cited in the following publications:
This publication cites the following data publications:

30 Citations in Europe PMC

Data provided by Europe PubMed Central.

Mutations and genomic islands can explain the strain dependency of sugar utilization in 21 strains of Propionibacterium freudenreichii.
Loux V, Mariadassou M, Almeida S, Chiapello H, Hammani A, Buratti J, Gendrault A, Barbe V, Aury JM, Deutsch SM, Parayre S, Madec MN, Chuat V, Jan G, Peterlongo P, Azevedo V, Le Loir Y, Falentin H., BMC Genomics 16(), 2015
PMID: 25886522
Investigation of ptsG gene in response to xylose utilization in Corynebacterium glutamicum.
Wang C, Cai H, Zhou Z, Zhang K, Chen Z, Chen Y, Wan H, Ouyang P., J. Ind. Microbiol. Biotechnol. 41(8), 2014
PMID: 24859809

31 References

Data provided by Europe PubMed Central.


AUTHOR UNKNOWN, 2004
Identification of two myo-inositol transporter genes of Bacillus subtilis.
Yoshida K, Yamamoto Y, Omae K, Yamamoto M, Fujita Y., J. Bacteriol. 184(4), 2002
PMID: 11807058
Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis.
Yoshida KI, Aoyama D, Ishio I, Shibayama T, Fujita Y., J. Bacteriol. 179(14), 1997
PMID: 9226270
Combined transcriptome and proteome analysis as a powerful approach to study genes under glucose repression in Bacillus subtilis.
Yoshida K, Kobayashi K, Miwa Y, Kang CM, Matsunaga M, Yamaguchi H, Tojo S, Yamamoto M, Nishi R, Ogasawara N, Nakayama T, Fujita Y., Nucleic Acids Res. 29(3), 2001
PMID: 11160890
The fifth gene of the iol operon of Bacillus subtilis, iolE, encodes 2-keto-myo-inositol dehydratase.
Yoshida K, Yamaguchi M, Ikeda H, Omae K, Tsurusaki K, Fujita Y., Microbiology (Reading, Engl.) 150(Pt 3), 2004
PMID: 14993306

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 16997948
PubMed | Europe PMC

Search this title in

Google Scholar