Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response

Kocan M, Schaffer S, Ishige T, Sorger-Herrmann U, Wendisch VF, Bott M (2006)
Journal of Bacteriology 188(2): 724-732.

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Abstract
Corynebacterium glutamicum contains genes for 13 two-component signal transduction systems. In order to test for their essentiality and involvement in the adaptive response to phosphate (Pi) starvation, a set of 12 deletion mutants was constructed. One of the mutants was specifically impaired in its ability to grow under Pi limitation, and therefore the genes lacking in this strain were named phoS (encoding the sensor kinase) and phoR (encoding the response regulator). DNA microarray analyses with the C glutamicum wild type and the Delta phoRS mutant supported a role for the PhoRS system in the adaptation to P, starvation. In contrast to the wild type, the Delta phoRS mutant did not induce the known Pi starvation-inducible (psi) genes within 1 hour after a shift from P, excess to Pi limitation, except for the pstSCAB operon, which was still partially induced. This indicates an activator function for PhoR and the existence of at least one additional regulator of the pst operon. Primer extension analysis of selected psi genes (pstS, ugpA, phoR, ushA, and nucH) confirmed the microarray data and provided evidence for positive autoregulation of the phoRS genes.
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Kocan M, Schaffer S, Ishige T, Sorger-Herrmann U, Wendisch VF, Bott M. Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response. Journal of Bacteriology. 2006;188(2):724-732.
Kocan, M., Schaffer, S., Ishige, T., Sorger-Herrmann, U., Wendisch, V. F., & Bott, M. (2006). Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response. Journal of Bacteriology, 188(2), 724-732.
Kocan, M., Schaffer, S., Ishige, T., Sorger-Herrmann, U., Wendisch, V. F., and Bott, M. (2006). Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response. Journal of Bacteriology 188, 724-732.
Kocan, M., et al., 2006. Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response. Journal of Bacteriology, 188(2), p 724-732.
M. Kocan, et al., “Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response”, Journal of Bacteriology, vol. 188, 2006, pp. 724-732.
Kocan, M., Schaffer, S., Ishige, T., Sorger-Herrmann, U., Wendisch, V.F., Bott, M.: Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response. Journal of Bacteriology. 188, 724-732 (2006).
Kocan, M., Schaffer, S., Ishige, T., Sorger-Herrmann, U., Wendisch, Volker F., and Bott, M. “Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response”. Journal of Bacteriology 188.2 (2006): 724-732.
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The Pho regulon: a huge regulatory network in bacteria.
Santos-Beneit F., Front Microbiol 6(), 2015
PMID: 25983732
Identification of Listeria monocytogenes determinants required for biofilm formation.
Alonso AN, Perry KJ, Regeimbal JM, Regan PM, Higgins DE., PLoS ONE 9(12), 2014
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Kretschmer M, Reiner E, Hu G, Tam N, Oliveira DL, Caza M, Yeon JH, Kim J, Kastrup CJ, Jung WH, Kronstad JW., Infect. Immun. 82(7), 2014
PMID: 24711572
The two-component system ChrSA is crucial for haem tolerance and interferes with HrrSA in haem-dependent gene regulation in Corynebacterium glutamicum.
Heyer A, Gatgens C, Hentschel E, Kalinowski J, Bott M, Frunzke J., Microbiology (Reading, Engl.) 158(Pt 12), 2012
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Arabitol metabolism of Corynebacterium glutamicum and its regulation by AtlR.
Laslo T, von Zaluskowski P, Gabris C, Lodd E, Ruckert C, Dangel P, Kalinowski J, Auchter M, Seibold G, Eikmanns BJ., J. Bacteriol. 194(5), 2012
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The two-component system PhoPR of Clostridium acetobutylicum is involved in phosphate-dependent gene regulation.
Fiedler T, Mix M, Meyer U, Mikkat S, Glocker MO, Bahl H, Fischer RJ., J. Bacteriol. 190(20), 2008
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Metabolic activity of Corynebacterium glutamicum grown on L: -lactic acid under stress.
Seletzky JM, Noack U, Fricke J, Hahn S, Buchs J., Appl. Microbiol. Biotechnol. 72(6), 2006
PMID: 16642330

34 References

Data provided by Europe PubMed Central.

The structure of the periplasmic ligand-binding domain of the sensor kinase CitA reveals the first extracellular PAS domain.
Reinelt S, Hofmann E, Gerharz T, Bott M, Madden DR., J. Biol. Chem. 278(40), 2003
PMID: 12867417
Binding of PhoP to promoters of phosphate-regulated genes in Streptomyces coelicolor: identification of PHO boxes.
Sola-Landa A, Rodriguez-Garcia A, Franco-Dominguez E, Martin JF., Mol. Microbiol. 56(5), 2005
PMID: 15882427

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