Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase

Georgi T, Rittmann D, Wendisch VF (2005)
Metabolic Engineering 7(4): 291-301.

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In the biotechnological production Of L-lysine and L-glutamate by Corynebacterium glutamicum media based on glucose, fructose or sucrose are typically used. Glutamate production by C glutamicum ATCC13032 was very similar on glucose, fructose, glucose plus fructose and sucrose. In contrast, lysine production of genetically defined C glutamicum strains was significantly higher on glucose than on the other carbon sources. To test whether malic enzyme or fructose-1,6-bisphosphatase might limit growth and lysine on fructose, glucose plus fructose or sucrose, strains overexpressing either malE which encodes the NADPH-dependent malic enzyme or the fructose-1,6-bisphosphatase gene fbp were generated. Overexpression of malE did not improve lysine production on any of the tested carbon sources. Upon overexpression of fbp lysine yields on glucose and/or fructose were unchanged, but the lysine yield on sucrose increased twofold. Thus, fructose-1,6-bisphosphatase was identified as a limiting factor for lysine production by C glutamicum with sucrose as the carbon source. (c) 2005 Elsevier Inc. All rights reserved.
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Georgi T, Rittmann D, Wendisch VF. Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metabolic Engineering. 2005;7(4):291-301.
Georgi, T., Rittmann, D., & Wendisch, V. F. (2005). Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metabolic Engineering, 7(4), 291-301.
Georgi, T., Rittmann, D., and Wendisch, V. F. (2005). Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metabolic Engineering 7, 291-301.
Georgi, T., Rittmann, D., & Wendisch, V.F., 2005. Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metabolic Engineering, 7(4), p 291-301.
T. Georgi, D. Rittmann, and V.F. Wendisch, “Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase”, Metabolic Engineering, vol. 7, 2005, pp. 291-301.
Georgi, T., Rittmann, D., Wendisch, V.F.: Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metabolic Engineering. 7, 291-301 (2005).
Georgi, T., Rittmann, D., and Wendisch, Volker F. “Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase”. Metabolic Engineering 7.4 (2005): 291-301.
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63 References

Data provided by Europe PubMed Central.

A functionally split pathway for lysine synthesis in Corynebacterium glutamicium.
Schrumpf B, Schwarzer A, Kalinowski J, Puhler A, Eggeling L, Sahm H., J. Bacteriol. 173(14), 1991
PMID: 1906065
Isolation and prominent characteristics of an l-lysine hyperproducing strain of Corynebacterium glutamicum
Schrumpf, Appl. Microbiol. Biotechnol. 37(), 1992
Concerted inhibition and its reversal by end products of aspartate kinase in Brevibacterium flavum
Shiio, J. Biochem. (Tokyo) 65(), 1969
Proposal for a new classification system, Actinobacteria classis nov
Stackebrandt, Int. J. Syst. Bacteriol. 47(), 1997
Network rigidity and metabolic engineering in metabolite overproduction.
Stephanopoulos G, Vallino JJ., Science 252(5013), 1991
PMID: 1904627
Regulation of 6-phosphogluconate dehydrogenase in Brevibacterium flavum
Sugimoto, Agric. Biol. Chem. 51(), 1987
Regulation of glucose-6-phosphate dehydrogenase in Brevibacterium flavum.
Sugimoto SI, Shiio I., Agric. Biol. Chem. 51(1), 1987
PMID: IND87014542
Cloning of a DNA fragment from Corynebacterium glutamicum conferring aminoethyl cysteine resistance and feedback resistance to aspartokinase.
Thierbach G, Kalinowski J, Bachmann B, Puhler A., Appl. Microbiol. Biotechnol. 32(4), 1990
PMID: 1366393
Global control of sugar metabolism: a gram-positive solution.
Titgemeyer F, Hillen W., Antonie Van Leeuwenhoek 82(1-4), 2002
PMID: 12369205

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