The Global Repressor SugR Controls Expression of Genes of Glycolysis and of the L-Lactate Dehydrogenase LdhA in Corynebacterium glutamicum

Engels V, Lindner S, Wendisch VF (2008)
Journal of Bacteriology 190(24): 8033-8044.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
The transcriptional regulator SugR from Corynebacterium glutamicum represses genes of the phosphoenol-pyruvate-dependent phosphotransferase system (PTS). Growth experiments revealed that the overexpression of sugR not only perturbed the growth of C. glutamicum on the PTS sugars glucose, fructose, and sucrose but also led to a significant growth inhibition on ribose, which is not taken up via the PTS. Chromatin immuno-precipitation combined with DNA microarray analysis and gel retardation experiments were performed to identify further target genes of SugR. Gel retardation analysis confirmed that SugR bound to the promoter regions of genes of the glycolytic enzymes 6-phosphofructokinase (pfkA), fructose-1,6-bisphosphate aldolase (fba), enolase (eno), pyruvate kinase (pyk), and NAD-dependent L-lactate dehydrogenase (ldhA). The deletion of sugR resulted in increased mRNA levels of eno, pyk, and ldhA in acetate medium. Enzyme activity measurements revealed that SugR-mediated repression affects the activities of PfkA, Fba, and LdhA in vivo. As the deletion of sugR led to increased LdhA activity under aerobic and under oxygen deprivation conditions, L-lactate production by C. glutamicum was determined. The overexpression of sugR reduced L-lactate production by about 25%, and sugR deletion increased L-lactate formation under oxygen deprivation conditions by threefold. Thus, SugR functions as a global repressor of genes of the PTS, glycolysis, and fermentative L-lactate dehydrogenase in C. glutamicum.
Publishing Year
ISSN
PUB-ID

Cite this

Engels V, Lindner S, Wendisch VF. The Global Repressor SugR Controls Expression of Genes of Glycolysis and of the L-Lactate Dehydrogenase LdhA in Corynebacterium glutamicum. Journal of Bacteriology. 2008;190(24):8033-8044.
Engels, V., Lindner, S., & Wendisch, V. F. (2008). The Global Repressor SugR Controls Expression of Genes of Glycolysis and of the L-Lactate Dehydrogenase LdhA in Corynebacterium glutamicum. Journal of Bacteriology, 190(24), 8033-8044.
Engels, V., Lindner, S., and Wendisch, V. F. (2008). The Global Repressor SugR Controls Expression of Genes of Glycolysis and of the L-Lactate Dehydrogenase LdhA in Corynebacterium glutamicum. Journal of Bacteriology 190, 8033-8044.
Engels, V., Lindner, S., & Wendisch, V.F., 2008. The Global Repressor SugR Controls Expression of Genes of Glycolysis and of the L-Lactate Dehydrogenase LdhA in Corynebacterium glutamicum. Journal of Bacteriology, 190(24), p 8033-8044.
V. Engels, S. Lindner, and V.F. Wendisch, “The Global Repressor SugR Controls Expression of Genes of Glycolysis and of the L-Lactate Dehydrogenase LdhA in Corynebacterium glutamicum”, Journal of Bacteriology, vol. 190, 2008, pp. 8033-8044.
Engels, V., Lindner, S., Wendisch, V.F.: The Global Repressor SugR Controls Expression of Genes of Glycolysis and of the L-Lactate Dehydrogenase LdhA in Corynebacterium glutamicum. Journal of Bacteriology. 190, 8033-8044 (2008).
Engels, V., Lindner, Steffen, and Wendisch, Volker F. “The Global Repressor SugR Controls Expression of Genes of Glycolysis and of the L-Lactate Dehydrogenase LdhA in Corynebacterium glutamicum”. Journal of Bacteriology 190.24 (2008): 8033-8044.
This data publication is cited in the following publications:
This publication cites the following data publications:

21 Citations in Europe PMC

Data provided by Europe PubMed Central.

Genome-wide analysis of the role of global transcriptional regulator GntR1 in Corynebacterium glutamicum.
Tanaka Y, Takemoto N, Ito T, Teramoto H, Yukawa H, Inui M., J. Bacteriol. 196(18), 2014
PMID: 24982307
Engineering of Corynebacterium glutamicum for growth and L-lysine and lycopene production from N-acetyl-glucosamine.
Matano C, Uhde A, Youn JW, Maeda T, Clermont L, Marin K, Kramer R, Wendisch VF, Seibold GM., Appl. Microbiol. Biotechnol. 98(12), 2014
PMID: 24668244
Adaptation of Bacillus subtilis carbon core metabolism to simultaneous nutrient limitation and osmotic challenge: a multi-omics perspective.
Kohlstedt M, Sappa PK, Meyer H, Maaß S, Zaprasis A, Hoffmann T, Becker J, Steil L, Hecker M, van Dijl JM, Lalk M, Mader U, Stulke J, Bremer E, Volker U, Wittmann C., Environ. Microbiol. 16(6), 2014
PMID: 24571712
Proteome response of Corynebacterium glutamicum to high concentration of industrially relevant C₄ and C₅ dicarboxylic acids.
Vasco-Cardenas MF, Banos S, Ramos A, Martin JF, Barreiro C., J Proteomics 85(), 2013
PMID: 23624027
Maltose uptake by the novel ABC transport system MusEFGK2I causes increased expression of ptsG in Corynebacterium glutamicum.
Henrich A, Kuhlmann N, Eck AW, Kramer R, Seibold GM., J. Bacteriol. 195(11), 2013
PMID: 23543710
Phosphotransferase system-mediated glucose uptake is repressed in phosphoglucoisomerase-deficient Corynebacterium glutamicum strains.
Lindner SN, Petrov DP, Hagmann CT, Henrich A, Kramer R, Eikmanns BJ, Wendisch VF, Seibold GM., Appl. Environ. Microbiol. 79(8), 2013
PMID: 23396334
Link between phosphate starvation and glycogen metabolism in Corynebacterium glutamicum, revealed by metabolomics.
Woo HM, Noack S, Seibold GM, Willbold S, Eikmanns BJ, Bott M., Appl. Environ. Microbiol. 76(20), 2010
PMID: 20802079
Integrating multiple 'omics' analysis for microbial biology: application and methodologies.
Zhang W, Li F, Nie L., Microbiology (Reading, Engl.) 156(Pt 2), 2010
PMID: 19910409

64 References

Data provided by Europe PubMed Central.


AUTHOR UNKNOWN, 2003
CcpA mutants with differential activities in Bacillus subtilis.
Sprehe M, Seidel G, Diel M, Hillen W., J. Mol. Microbiol. Biotechnol. 12(1-2), 2007
PMID: 17183216

AUTHOR UNKNOWN, 1997
Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production.
Stansen C, Uy D, Delaunay S, Eggeling L, Goergen JL, Wendisch VF., Appl. Environ. Microbiol. 71(10), 2005
PMID: 16204505

AUTHOR UNKNOWN, 1989

AUTHOR UNKNOWN, 1989
Regulation of the expression of phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS) genes in Corynebacterium glutamicum R.
Tanaka Y, Okai N, Teramoto H, Inui M, Yukawa H., Microbiology (Reading, Engl.) 154(Pt 1), 2008
PMID: 18174145
Role of CcpA in regulation of the central pathways of carbon catabolism in Bacillus subtilis.
Tobisch S, Zuhlke D, Bernhardt J, Stulke J, Hecker M., J. Bacteriol. 181(22), 1999
PMID: 10559165

AUTHOR UNKNOWN, 2006

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 18849435
PubMed | Europe PMC

Search this title in

Google Scholar