Genetic engineering of the multicellular green alga Volvox: a modified and multiplied bacterial antibiotic resistance gene as a dominant selectable marker

Hallmann A, Rappel A (1999)
Plant J. 17(1): 99-109.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
;
Abstract
The green alga Volvox represents the simplest multicellular organism: Volvox is composed of only two cell types, somatic and reproductive. Volvox, therefore, is an attractive model system for studying various aspects of multicellularity. With the biolistic nuclear transformation of Volvox carteri, the powerful molecular genetic manipulation of this organism has been established, but applications have been restricted to an auxotrophic mutant serving as the DNA recipient. Therefore, a dominant selectable marker working in all strains and mutants of this organism is required. Among several gene constructs tested, the most advantageous results were obtained with a chimeric gene composed of the coding sequence of the bacterial ble gene, conferring resistance to the antibiotic zeocin, modified with insertions of two endogenous introns from the Volvox arylsulfatase gene and fused to 5' and 3' untranslated regions from the Volvox beta 2-tubulin gene. In the most suitable plasmid used, the gene dosage was increased 16-fold by a technique that allows exponential multiplication of a DNA fragment. Co-transformation of this plasmid and a non-selectable plasmid allowed the identification of zeocin resistant transformants with nuclear integration of both selectable and non-selectable plasmids. Stable expression of the ble gene and of genes from several non-selectable plasmids is demonstrated. The modified ble gene provides the first dominant marker for transformation of both wild-type and mutant strains of Volvox.
Publishing Year
ISSN
PUB-ID

Cite this

Hallmann A, Rappel A. Genetic engineering of the multicellular green alga Volvox: a modified and multiplied bacterial antibiotic resistance gene as a dominant selectable marker. Plant J. 1999;17(1):99-109.
Hallmann, A., & Rappel, A. (1999). Genetic engineering of the multicellular green alga Volvox: a modified and multiplied bacterial antibiotic resistance gene as a dominant selectable marker. Plant J., 17(1), 99-109.
Hallmann, A., and Rappel, A. (1999). Genetic engineering of the multicellular green alga Volvox: a modified and multiplied bacterial antibiotic resistance gene as a dominant selectable marker. Plant J. 17, 99-109.
Hallmann, A., & Rappel, A., 1999. Genetic engineering of the multicellular green alga Volvox: a modified and multiplied bacterial antibiotic resistance gene as a dominant selectable marker. Plant J., 17(1), p 99-109.
A. Hallmann and A. Rappel, “Genetic engineering of the multicellular green alga Volvox: a modified and multiplied bacterial antibiotic resistance gene as a dominant selectable marker”, Plant J., vol. 17, 1999, pp. 99-109.
Hallmann, A., Rappel, A.: Genetic engineering of the multicellular green alga Volvox: a modified and multiplied bacterial antibiotic resistance gene as a dominant selectable marker. Plant J. 17, 99-109 (1999).
Hallmann, Armin, and Rappel, A. “Genetic engineering of the multicellular green alga Volvox: a modified and multiplied bacterial antibiotic resistance gene as a dominant selectable marker”. Plant J. 17.1 (1999): 99-109.
This data publication is cited in the following publications:
This publication cites the following data publications:

18 Citations in Europe PMC

Data provided by Europe PubMed Central.

Transgene Expression in Microalgae-From Tools to Applications.
Doron L, Segal N, Shapira M., Front Plant Sci 7(), 2016
PMID: 27148328
The Potential for Microalgae as Bioreactors to Produce Pharmaceuticals.
Yan N, Fan C, Chen Y, Hu Z., Int J Mol Sci 17(6), 2016
PMID: 27322258
The inducible nitA promoter provides a powerful molecular switch for transgene expression in Volvox carteri.
von der Heyde EL, Klein B, Abram L, Hallmann A., BMC Biotechnol 15(), 2015
PMID: 25888095
Stable nuclear transformation of Pandorina morum.
Lerche K, Hallmann A., BMC Biotechnol 14(), 2014
PMID: 25031031
Stable nuclear transformation of Eudorina elegans.
Lerche K, Hallmann A., BMC Biotechnol 13(), 2013
PMID: 23402598
Physical methods for genetic plant transformation.
Rivera AL, Gómez-Lim M, Fernández F, Loske AM., Phys Life Rev 9(3), 2012
PMID: 22704230
Evolution of reproductive development in the volvocine algae.
Hallmann A., Sex Plant Reprod 24(2), 2011
PMID: 21174128
Microalgae as platforms for production of recombinant proteins and valuable compounds: progress and prospects.
Gong Y, Hu H, Gao Y, Xu X, Gao H., J Ind Microbiol Biotechnol 38(12), 2011
PMID: 21882013
Modifications of the metabolic pathways of lipid and triacylglycerol production in microalgae.
Yu WL, Ansari W, Schoepp NG, Hannon MJ, Mayfield SP, Burkart MD., Microb Cell Fact 10(), 2011
PMID: 22047615
Biofuels from algae: challenges and potential.
Hannon M, Gimpel J, Tran M, Rasala B, Mayfield S., Biofuels 1(5), 2010
PMID: 21833344
Stable nuclear transformation of Gonium pectorale.
Lerche K, Hallmann A., BMC Biotechnol 9(), 2009
PMID: 19591675
Characterization of a heat-shock-inducible hsp70 gene of the green alga Volvox carteri.
Cheng Q, Hallmann A, Edwards L, Miller SM., Gene 371(1), 2006
PMID: 16476527
Hsp70A and GlsA interact as partner chaperones to regulate asymmetric division in Volvox.
Cheng Q, Pappas V, Hallmann A, Miller SM., Dev Biol 286(2), 2005
PMID: 16168403
Cell-death alternative model organisms: why and which?
Golstein P, Aubry L, Levraud JP., Nat Rev Mol Cell Biol 4(10), 2003
PMID: 14570057
Differentiation of germinal and somatic cells in Volvox carteri.
Schmitt R., Curr Opin Microbiol 6(6), 2003
PMID: 14662357
Evolution of multicellularity in the volvocine algae.
Kirk DL., Curr Opin Plant Biol 2(6), 1999
PMID: 10607653

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 10069071
PubMed | Europe PMC

Search this title in

Google Scholar