Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri

Nematollahi G, Kianianmomeni A, Hallmann A (2006)
BMC Genomics 7(1): 321.

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Abstract
Background: The multicellular alga Volvox carteri possesses only two cell types: mortal, motile somatic cells and potentially immortal, immotile reproductive cells. It is therefore an attractive model system for studying how cell-autonomous cytodifferentiation is programmed within a genome. Moreover, there are ongoing genome projects both in Volvox carteri and in the closely related unicellular alga Chlamydomonas reinhardtii. However, gene sequencing is only the beginning. To identify cell-type specific expression and to determine relative expression rates, we evaluate the potential of real-time RT-PCR for quantifying gene transcript levels. Results: Here we analyze a diversified pool of 39 target genes by real-time RT-PCR for each cell type. This gene pool contains previously known genes with unknown localization of cellular expression, 28 novel genes which are described in this study for the first time, and a few known, cell-type specific genes as a control. The respective gene products are, for instance, part of photosynthesis, cellular regulation, stress response, or transport processes. We provide expression data for all these genes. Conclusion: The results show that quantitative real-time RT-PCR is a favorable approach to analyze cell-type specific gene expression in Volvox, which can be extended to a much larger number of genes or to developmental or metabolic mutants. Our expression data also provide a basis for a detailed analysis of individual, previously unknown, cell-type specifically expressed genes.
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Nematollahi G, Kianianmomeni A, Hallmann A. Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri. BMC Genomics. 2006;7(1): 321.
Nematollahi, G., Kianianmomeni, A., & Hallmann, A. (2006). Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri. BMC Genomics, 7(1): 321.
Nematollahi, G., Kianianmomeni, A., and Hallmann, A. (2006). Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri. BMC Genomics 7:321.
Nematollahi, G., Kianianmomeni, A., & Hallmann, A., 2006. Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri. BMC Genomics, 7(1): 321.
G. Nematollahi, A. Kianianmomeni, and A. Hallmann, “Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri”, BMC Genomics, vol. 7, 2006, : 321.
Nematollahi, G., Kianianmomeni, A., Hallmann, A.: Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri. BMC Genomics. 7, : 321 (2006).
Nematollahi, Ghazaleh, Kianianmomeni, Arash, and Hallmann, Armin. “Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri”. BMC Genomics 7.1 (2006): 321.
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14 Citations in Europe PMC

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PMID: 25516378
MicroRNAs in a multicellular green alga Volvox carteri.
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PMID: 24369344
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Kianianmomeni A, Hallmann A., Planta 239(1), 2014
PMID: 24081482
Building blocks of the nexin-dynein regulatory complex in Chlamydomonas flagella.
Lin J, Tritschler D, Song K, Barber CF, Cobb JS, Porter ME, Nicastro D., J. Biol. Chem. 286(33), 2011
PMID: 21700706
Architecture and organization of chicken microchromosome 16: order of the NOR, MHC-Y, and MHC-B subregions.
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PMID: 19617522
Translational control of photosynthetic gene expression in phototrophic eukaryotes
Wobbe L, Schwarz C, Nickelsen J, Kruse O., Physiol Plant 133(3), 2008
PMID: IND44069756
Translational control of photosynthetic gene expression in phototrophic eukaryotes.
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PMID: 18346070

61 References

Data provided by Europe PubMed Central.

NCBI BLAST
AUTHOR UNKNOWN, 0
DOE Joint Genome Institute (JGI)
AUTHOR UNKNOWN, 0
Cloning and characterization of the actin-encoding gene of Chlamydomonas reinhardtii.
Sugase Y, Hirono M, Kindle KL, Kamiya R., Gene 168(1), 1996
PMID: 8626057
Volvox germline-specific genes that are putative targets of RegA repression encode chloroplast proteins.
Meissner M, Stark K, Cresnar B, Kirk DL, Schmitt R., Curr. Genet. 36(6), 1999
PMID: 10654090
Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii.
Fernandez E, Schnell R, Ranum LP, Hussey SC, Silflow CD, Lefebvre PA., Proc. Natl. Acad. Sci. U.S.A. 86(17), 1989
PMID: 2475871
A cDNA clone encoding Chlamydomonas reinhardtii preferredoxin.
Stein M, Jacquot JP, Miginiac-Maslow M., Plant Physiol. 102(4), 1993
PMID: 8278553

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