Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system

Hartmann U, Valentine WJ, Christie JM, Hays J, Jenkins GI, Weisshaar B (1998)
Plant Molecular Biology 36(5): 741-754.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ;
Abstract / Bemerkung
To identify DNA sequences of the Arabidopsis thaliana chalcone synthase gene (CHS) concerned with induction by UV-B and UV-A/blue light, AtCHS promoter constructions were assayed by transient expression in protoplasts prepared from two different lines of cultured A. thaliana cells. The protoplasts responded similarly to A. thaliana leaf tissue in light-dependent CHS transcript accumulation. The reporter enzyme beta-glucuronidase (GUS) was used to monitor light-responsive promoter activity. A 1972 bp promoter conferred UV-B and UV-A/blue light induction of GUS activity. Deletion to 164 bp resulted in reduced promoter strength but retention of responsiveness to UV-B and UV-A/blue light. Further deletion abolished transcriptional activity. The 164 bp promoter contains sequences closely resembling LRUPcCHS, (light-responsive unit of the Petroselinum crispum CHS promoter). This A. thaliana CHS promoter region, designated LRUAtCHS, was sufficient to confer UV-B and UV-A/blue light responsiveness to a heterologous core promoter. Mutation of sequences in LRUAtCHS corresponding to the ACGT element and the MYB recognition element of LRUPcCHS resulted in inactivation of the 164 bp and 335 bp promoter deletions. However, the mutant 668 bp promoter retained residual UV-B and UV-A/blue light-induced expression, indicating the presence of additional functional sequences upstream of -335. Mutation of a single G-box-like sequence around -442 had no effect on light responsiveness, indicating that it does not function in light regulation of this promoter. Since no difference in responsiveness to UV-B and UV-A/blue light was observed with any promoter variant, we conclude that the two phototransduction pathways regulate transcription factors which interact with common promoter elements. The results from-our analysis of a A. thaliana light-responsive promoter will facilitate the study of light-dependent gene regulation by genetic means in Arabidopsis thaliana.
Erscheinungsjahr
Zeitschriftentitel
Plant Molecular Biology
Band
36
Zeitschriftennummer
5
Seite
741-754
ISSN
PUB-ID

Zitieren

Hartmann U, Valentine WJ, Christie JM, Hays J, Jenkins GI, Weisshaar B. Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology. 1998;36(5):741-754.
Hartmann, U., Valentine, W. J., Christie, J. M., Hays, J., Jenkins, G. I., & Weisshaar, B. (1998). Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology, 36(5), 741-754. doi:10.1023/A:1005921914384
Hartmann, U., Valentine, W. J., Christie, J. M., Hays, J., Jenkins, G. I., and Weisshaar, B. (1998). Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology 36, 741-754.
Hartmann, U., et al., 1998. Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology, 36(5), p 741-754.
U. Hartmann, et al., “Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system”, Plant Molecular Biology, vol. 36, 1998, pp. 741-754.
Hartmann, U., Valentine, W.J., Christie, J.M., Hays, J., Jenkins, G.I., Weisshaar, B.: Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology. 36, 741-754 (1998).
Hartmann, U., Valentine, W.J., Christie, J.M., Hays, J, Jenkins, G.I., and Weisshaar, Bernd. “Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system”. Plant Molecular Biology 36.5 (1998): 741-754.

72 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Genome-wide investigation of light and carbon signaling interactions in Arabidopsis.
Thum KE, Shin MJ, Palenchar PM, Kouranov A, Coruzzi GM., Genome Biol 5(2), 2004
PMID: 14759260
Characterization and structural features of a chalcone synthase mutation in a white-flowering line of Matthiola incana R. Br. (Brassicaceae).
Hemleben V, Dressel A, Epping B, Lukacin R, Martens S, Austin M., Plant Mol Biol 55(3), 2004
PMID: 15604692
TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana.
Baudry A, Heim MA, Dubreucq B, Caboche M, Weisshaar B, Lepiniec L., Plant J 39(3), 2004
PMID: 15255866
Construction of gene expression system in hop (Humulus lupulus) lupulin gland using valerophenone synthase promoter.
Okada Y, Saeki K, Inaba A, Suda N, Kaneko T, Ito K., J Plant Physiol 160(9), 2003
PMID: 14593812
Targets of AtWRKY6 regulation during plant senescence and pathogen defense.
Robatzek S, Somssich IE., Genes Dev 16(9), 2002
PMID: 12000796
Gene regulation by low level UV-B radiation: identification by DNA array analysis.
Brosché M, Schuler MA, Kalbina I, Connor L, Strid A., Photochem Photobiol Sci 1(9), 2002
PMID: 12665302
Expression of Arabidopsis LINEs from two promoters.
Ohta Y, Noma K, Tsuchimoto S, Ohtsubo E, Ohtsubo H., Plant J 32(5), 2002
PMID: 12472695
Genetic dissection of blue-light sensing in tomato using mutants deficient in cryptochrome 1 and phytochromes A, B1 and B2.
Weller JL, Perrotta G, Schreuder ME, van Tuinen A, Koornneef M, Giuliano G, Kendrick RE., Plant J 25(4), 2001
PMID: 11260499
UV and blue light signalling: pathways regulating chalcone synthase gene expression in Arabidopsis.
Jenkins GI, Long JC, Wade HK, Shenton MR, Bibikova TN., New Phytol 151(1), 2001
PMID: IND23226844
Comparative genomics and regulatory evolution: conservation and function of the Chs and Apetala3 promoters.
Koch MA, Weisshaar B, Kroymann J, Haubold B, Mitchell-Olds T., Mol Biol Evol 18(10), 2001
PMID: 11557794
UVB/UVA radiation activates a 48 kDa myelin basic protein kinase and potentiates wound signaling in tomato leaves.
Stratmann JW, Stelmach BA, Weiler EW, Ryan CA., Photochem Photobiol 71(2), 2000
PMID: 10687382
Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis.
Jin H, Cominelli E, Bailey P, Parr A, Mehrtens F, Jones J, Tonelli C, Weisshaar B, Martin C., EMBO J 19(22), 2000
PMID: 11080161
C4 GENE EXPRESSION.
Sheen J., Annu Rev Plant Physiol Plant Mol Biol 50(), 1999
PMID: 15012208
Three 4-coumarate:coenzyme A ligases in Arabidopsis thaliana represent two evolutionarily divergent classes in angiosperms.
Ehlting J, Büttner D, Wang Q, Douglas CJ, Somssich IE, Kombrink E., Plant J 19(1), 1999
PMID: 10417722
Phenylpropanoid biosynthesis and its regulation.
Weisshaar B, Jenkins GI., Curr Opin Plant Biol 1(3), 1998
PMID: 10066590
Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes.
Wisman E, Hartmann U, Sagasser M, Baumann E, Palme K, Hahlbrock K, Saedler H, Weisshaar B., Proc Natl Acad Sci U S A 95(21), 1998
PMID: 9770503

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 9526507
PubMed | Europe PMC

Suchen in

Google Scholar