TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana

Baudry A, Heim MA, Dubreucq B, Caboche M, Weisshaar B, Lepiniec L (2004)
The Plant Journal 39(3): 366-380.

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Abstract
Genetic analyses have demonstrated that together with TTG1, a WD-repeat (WDR) protein, TT2 (MYB), and TT8 (bHLH) are necessary for the correct expression of BANYULS (BAN). This gene codes for the core enzyme of proanthocyanidin biosynthesis in Arabidopsis thaliana seed coat. The interplays of TT2, TT8, and their closest MYB/bHLH relatives, with TTG1 and the BAN promoter have been investigated using a combination of genetic and molecular approaches, both in yeast and in planta. The results obtained using glucocorticoid receptor fusion proteins in planta strongly suggest that TT2, TT8, and TTG1 can directly activate BAN expression. Experiments using yeast two- and three-hybrid clearly demonstrated that TT2, TT8, and TTG1 can form a stable ternary complex. Furthermore, although TT2 and TT8 were able to bind to the BAN promoter when simultaneously expressed in yeast, the activity of the complex correlated with the level of TTG1 expression in A. thaliana protoplasts. In addition, transient expression experiments revealed that TTG1 acts mainly through the bHLH partner (i.e. TT8 or related proteins) and that TT2 cannot be replaced by any other related A. thaliana MYB proteins to activate BAN. Finally and consistent with these results, the ectopic expression of TT2 was sufficient to trigger BAN activation in vegetative parts, but only where TTG1 was expressed. Taken together, these results indicate that TT2, TT8, and TTG1 can form a ternary complex directly regulating BAN expression in planta.
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Baudry A, Heim MA, Dubreucq B, Caboche M, Weisshaar B, Lepiniec L. TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana. The Plant Journal. 2004;39(3):366-380.
Baudry, A., Heim, M. A., Dubreucq, B., Caboche, M., Weisshaar, B., & Lepiniec, L. (2004). TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana. The Plant Journal, 39(3), 366-380.
Baudry, A., Heim, M. A., Dubreucq, B., Caboche, M., Weisshaar, B., and Lepiniec, L. (2004). TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana. The Plant Journal 39, 366-380.
Baudry, A., et al., 2004. TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana. The Plant Journal, 39(3), p 366-380.
A. Baudry, et al., “TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana”, The Plant Journal, vol. 39, 2004, pp. 366-380.
Baudry, A., Heim, M.A., Dubreucq, B., Caboche, M., Weisshaar, B., Lepiniec, L.: TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana. The Plant Journal. 39, 366-380 (2004).
Baudry, A., Heim, Marc A., Dubreucq, B., Caboche, M., Weisshaar, Bernd, and Lepiniec, L. “TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana”. The Plant Journal 39.3 (2004): 366-380.
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65 References

Data provided by Europe PubMed Central.

The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.
Harper JW, Adami GR, Wei N, Keyomarsi K, Elledge SJ., Cell 75(4), 1993
PMID: 8242751
Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development.
Ang LH, Chattopadhyay S, Wei N, Oyama T, Okada K, Batschauer A, Deng XW., Mol. Cell 1(2), 1998
PMID: 9659918
Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens.
Fromont-Racine M, Rain JC, Legrain P., Nat. Genet. 16(3), 1997
PMID: 9207794
A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules.
Oppenheimer DG, Herman PL, Sivakumaran S, Esch J, Marks MD., Cell 67(3), 1991
PMID: 1934056
The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector
Koncz, MGG Molecular & General Genetics 204(3), 1986
Arabidopsis and Nicotiana anthocyanin production activated by maize regulators R and C1.
Lloyd AM, Walbot V, Davis RW., Science 258(5089), 1992
PMID: 1465611
Epidermal cell fate determination in Arabidopsis: patterns defined by a steroid-inducible regulator.
Lloyd AM, Schena M, Walbot V, Davis RW., Science 266(5184), 1994
PMID: 7939683
Flavonoids in seeds and grains: physiological function, agronomic importance and the genetics of biosynthesis
Shirley, Seed Science Research 8(4), 1998

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