Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana
Köhler C, Merkle T, Neuhaus G (1999)
The Plant Journal 18(1): 97-104.
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In plants, cyclic GMP is involved in signal transduction in response to light and gibberellic acid. For cyclic AMP, a potential role during the plant cell cycle was recently reported. However, cellular targets for cyclic nucleotides in plants are largely unknown. Here we report on the identification and characterisation of a new gene family in Arabidopsis, which share features with cyclic nucleotide-gated channels from animals and inward-rectifying K+ channels from plants. The identified gene family comprises six members (Arabidopsis thaliana cyclic nucleotide-gated channels, AtCNGC1-6) with significant homology among the deduced proteins. Hydrophobicity analysis predicted six membrane-spanning domains flanked by hydrophilic amino and carboxy termini. A putative cyclic nucleotide binding domain (CNBD) which contains several residues that are invariant in other CNBDs was located in the carboxy terminus. This domain overlaps with a predicted calmodulin (CaM) binding site, suggesting interaction between cyclic nucleotide and CaM regulation. We demonstrated interaction of the carboxy termini of AtCNGC1 and AtCNGC2 with CaM in yeast, indicating that the CaM binding sites are functional. Furthermore, it was shown that both AtCNGC1 and AtCNGC2 can partly complement the K(+)-uptake-deficient yeast mutant CY162. Therefore, we propose that the identified genes constitute a family of plant cyclic nucleotide- and CaM-regulated ion channels.
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Köhler C, Merkle T, Neuhaus G. Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. The Plant Journal. 1999;18(1):97-104.
Köhler, C., Merkle, T., & Neuhaus, G. (1999). Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. The Plant Journal, 18(1), 97-104. doi:10.1046/j.1365-313X.1999.00422.x
Köhler, C., Merkle, T., and Neuhaus, G. (1999). Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. The Plant Journal 18, 97-104.
Köhler, C., Merkle, T., & Neuhaus, G., 1999. Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. The Plant Journal, 18(1), p 97-104.
C. Köhler, T. Merkle, and G. Neuhaus, “Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana”, The Plant Journal, vol. 18, 1999, pp. 97-104.
Köhler, C., Merkle, T., Neuhaus, G.: Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. The Plant Journal. 18, 97-104 (1999).
Köhler, C., Merkle, Thomas, and Neuhaus, G. “Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana”. The Plant Journal 18.1 (1999): 97-104.
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10 Items found citing this article
Cyclic nucleotide-gated ion channel 4 (UNIPROT: Q94AS9)
Organism: Arabidopsis thaliana
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Organism: Arabidopsis thaliana
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Probable cyclic nucleotide-gated ion channel 6 (UNIPROT: O82226)
Organism: Arabidopsis thaliana
Download in FASTA format
Organism: Arabidopsis thaliana
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Cyclic nucleotide-gated ion channel 2 (UNIPROT: O65718)
Organism: Arabidopsis thaliana
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Organism: Arabidopsis thaliana
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Cyclic nucleotide-gated ion channel 4 (UNIPROT: F4K0A1)
Organism: Arabidopsis thaliana
Download in FASTA format
Organism: Arabidopsis thaliana
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Cyclic nucleotide-gated ion channel 2 (UNIPROT: F4K9S7)
Organism: Arabidopsis thaliana
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Organism: Arabidopsis thaliana
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Probable cyclic nucleotide-gated ion channel 5 (UNIPROT: Q8RWS9)
Organism: Arabidopsis thaliana
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Organism: Arabidopsis thaliana
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Cyclic nucleotide-gated ion channel 1 (UNIPROT: O65717)
Organism: Arabidopsis thaliana
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Organism: Arabidopsis thaliana
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Probable cyclic nucleotide-gated ion channel 3 (UNIPROT: Q9SKD7)
Organism: Arabidopsis thaliana
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Organism: Arabidopsis thaliana
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53 Citations in Europe PMC
Data provided by Europe PubMed Central.
Characterisation of calmodulin binding to cyclic nucleotide-gated ion channels from Arabidopsis thaliana.
Köhler C, Neuhaus G., FEBS Lett 471(2-3), 2000
PMID: 10767408
Köhler C, Neuhaus G., FEBS Lett 471(2-3), 2000
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A tobacco plasma membrane calmodulin-binding transporter confers Ni2+ tolerance and Pb2+ hypersensitivity in transgenic plants.
Arazi T, Sunkar R, Kaplan B, Fromm H., Plant J 20(2), 1999
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Arazi T, Sunkar R, Kaplan B, Fromm H., Plant J 20(2), 1999
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Plant ion channels: from molecular structures to physiological functions.
Zimmermann S, Sentenac H., Curr Opin Plant Biol 2(6), 1999
PMID: 10607654
Zimmermann S, Sentenac H., Curr Opin Plant Biol 2(6), 1999
PMID: 10607654
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