Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana

Köhler C, Merkle T, Neuhaus G (1999)
The Plant Journal 18(1): 97-104.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ;
Abstract / Bemerkung
In plants, cyclic GMP is involved in signal transduction in response to light and gibberellic acid. For cyclic AMP, a potential role during the plant cell cycle was recently reported. However, cellular targets for cyclic nucleotides in plants are largely unknown. Here we report on the identification and characterisation of a new gene family in Arabidopsis, which share features with cyclic nucleotide-gated channels from animals and inward-rectifying K+ channels from plants. The identified gene family comprises six members (Arabidopsis thaliana cyclic nucleotide-gated channels, AtCNGC1-6) with significant homology among the deduced proteins. Hydrophobicity analysis predicted six membrane-spanning domains flanked by hydrophilic amino and carboxy termini. A putative cyclic nucleotide binding domain (CNBD) which contains several residues that are invariant in other CNBDs was located in the carboxy terminus. This domain overlaps with a predicted calmodulin (CaM) binding site, suggesting interaction between cyclic nucleotide and CaM regulation. We demonstrated interaction of the carboxy termini of AtCNGC1 and AtCNGC2 with CaM in yeast, indicating that the CaM binding sites are functional. Furthermore, it was shown that both AtCNGC1 and AtCNGC2 can partly complement the K(+)-uptake-deficient yeast mutant CY162. Therefore, we propose that the identified genes constitute a family of plant cyclic nucleotide- and CaM-regulated ion channels.
Erscheinungsjahr
Zeitschriftentitel
The Plant Journal
Band
18
Zeitschriftennummer
1
Seite
97-104
ISSN
eISSN
PUB-ID

Zitieren

Köhler C, Merkle T, Neuhaus G. Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. The Plant Journal. 1999;18(1):97-104.
Köhler, C., Merkle, T., & Neuhaus, G. (1999). Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. The Plant Journal, 18(1), 97-104. doi:10.1046/j.1365-313X.1999.00422.x
Köhler, C., Merkle, T., and Neuhaus, G. (1999). Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. The Plant Journal 18, 97-104.
Köhler, C., Merkle, T., & Neuhaus, G., 1999. Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. The Plant Journal, 18(1), p 97-104.
C. Köhler, T. Merkle, and G. Neuhaus, “Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana”, The Plant Journal, vol. 18, 1999, pp. 97-104.
Köhler, C., Merkle, T., Neuhaus, G.: Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. The Plant Journal. 18, 97-104 (1999).
Köhler, C., Merkle, Thomas, and Neuhaus, G. “Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana”. The Plant Journal 18.1 (1999): 97-104.

53 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Plant ion channels: from molecular structures to physiological functions.
Zimmermann S, Sentenac H., Curr Opin Plant Biol 2(6), 1999
PMID: 10607654

35 References

Daten bereitgestellt von Europe PubMed Central.


AUTHOR UNKNOWN, 0
Cloning and expression in yeast of a plant potassium ion transport system.
Sentenac H, Bonneaud N, Minet M, Lacroute F, Salmon JM, Gaymard F, Grignon C., Science 256(5057), 1992
PMID: 1585180
Cyclic nucleotide-binding domains in proteins having diverse functions.
Shabb JB, Corbin JD., J. Biol. Chem. 267(9), 1992
PMID: 1313416
Guanosine cyclic 3',5'-phosphate dependent protein kinase, a chimeric protein homologous with two separate protein families.
Takio K, Wade RD, Smith SB, Krebs EG, Walsh KA, Titani K., Biochemistry 23(18), 1984
PMID: 6091741
Amino acid sequence of the regulatory subunit of bovine type I adenosine cyclic 3',5'-phosphate dependent protein kinase.
Titani K, Sasagawa T, Ericsson LH, Kumar S, Smith SB, Krebs EG, Walsh KA., Biochemistry 23(18), 1984
PMID: 6487597
A distinct potassium channel polypeptide encoded by the Drosophila eag locus.
Warmke J, Drysdale R, Ganetzky B., Science 252(5012), 1991
PMID: 1840699
Calmodulin controls the rod photoreceptor CNG channel through an unconventional binding site in the N-terminus of the beta-subunit.
Weitz D, Zoche M, Muller F, Beyermann M, Korschen HG, Kaupp UB, Koch KW., EMBO J. 17(8), 1998
PMID: 9545240

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 10341447
PubMed | Europe PMC

Suchen in

Google Scholar