Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation.

Obrdlik P, Neuhaus G, Merkle T (2000)
FEBS Letters 476(3): 208-212.

Download
No fulltext has been uploaded. References only!
DOI
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ;
Department
Fakultät für Biologie > Genomforschung
Abstract
Gbeta subunits from animals are anchored to membranes via Ggamma subunits. No Ggamma has been identified in plants to date. Using differential centrifugation of Arabidopsis and broccoli extracts, Gbeta was highly enriched in the microsomal pellet. Treatment of microsomes with detergents and salts indicates that plant Gbeta is located at the membrane surface and attached to membranes by hydrophobic interactions. Analysis of transgenic plants expressing Gbeta-GFP fusion proteins showed that mutations in the heptad repeat domain of Gbeta severely diminished their membrane association. We propose that plant Gbeta is anchored to membranes by an unknown protein similar to animal Gbeta by Ggamma, via coiled-coil formation.
Publishing Year
2000
ISSN
0014-5793
PUB-ID

Cite this

Obrdlik P, Neuhaus G, Merkle T. Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Letters. 2000;476(3):208-212.
Obrdlik, P., Neuhaus, G., & Merkle, T. (2000). Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Letters, 476(3), 208-212. doi:10.1016/S0014-5793(00)01706-3
Obrdlik, P., Neuhaus, G., and Merkle, T. (2000). Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Letters 476, 208-212.
Obrdlik, P., Neuhaus, G., & Merkle, T., 2000. Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Letters, 476(3), p 208-212.
P. Obrdlik, G. Neuhaus, and T. Merkle, “Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation.”, FEBS Letters, vol. 476, 2000, pp. 208-212.
Obrdlik, P., Neuhaus, G., Merkle, T.: Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation. FEBS Letters. 476, 208-212 (2000).
Obrdlik, P., Neuhaus, G., and Merkle, Thomas. “Plant heterotrimeric G protein beta subunit is associated with membranes via protein interactions involving coiled-coil formation.”. FEBS Letters 476.3 (2000): 208-212.
This data publication is cited in the following publications:
This publication cites the following data publications:

10 Citations in Europe PMC

Data provided by Europe PubMed Central.

Roles of an N-terminal coiled-coil-containing domain in the localization and function of Bem3, a Rho GTPase-activating protein in budding yeast.
Nie WC, He F, Yuan SM, Jia ZW, Wang RR, Gao XD., Fungal Genet Biol 99(), 2017
PMID: 28064039
Two-state conformational equilibrium in the Par-4 leucine zipper domain.
Schwalbe M, Dutta K, Libich DS, Venugopal H, Claridge JK, Gell DA, Mackay JP, Edwards PJ, Pascal SM., Proteins 78(11), 2010
PMID: 20602362
Arabidopsis extra large G-protein 2 (XLG2) interacts with the Gbeta subunit of heterotrimeric G protein and functions in disease resistance.
Zhu H, Li GJ, Ding L, Cui X, Berg H, Assmann SM, Xia Y., Mol Plant 2(3), 2009
PMID: 19825634
Characterization of the Arabidopsis heterotrimeric G protein.
Wang S, Assmann SM, Fedoroff NV., J Biol Chem 283(20), 2008
PMID: 18326491
Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein.
Shen Y, Han YJ, Kim JI, Song PS., BMB Rep 41(9), 2008
PMID: 18823588
Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein beta-subunit AGB1.
Anderson DJ, Botella JR., Plant Cell Rep 26(9), 2007
PMID: 17492287
Effects of urea pretreatment on the binding properties of adenosine A1 receptors.
May LT, Sexton PM, Christopoulos A., Br J Pharmacol 146(8), 2005
PMID: 16231004
Lipid-membrane affinity of chimeric metal-binding green fluorescent protein.
Prachayasittikul V, Isarankura Na Ayudhya C, Boonpangrak S, Galla HJ., J Membr Biol 200(1), 2004
PMID: 15386159
The response regulator 2 mediates ethylene signalling and hormone signal integration in Arabidopsis.
Hass C, Lohrmann J, Albrecht V, Sweere U, Hummel F, Yoo SD, Hwang I, Zhu T, Schäfer E, Kudla J, Harter K., EMBO J 23(16), 2004
PMID: 15282545
Heterotrimeric G-proteins in plant cell signaling.
Millner PA., New Phytol 151(1), 2001
PMID: IND23226852

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 10913615
PubMed | Europe PMC

Search this title in

Google Scholar