DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize.

Röttgers K, Krohn NM, Lichota J, Stemmer C, Merkle T, Grasser KD (2000)
The Plant Journal 23(3): 395-405.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ;
Abstract
The structure-specific recognition protein 1 (SSRP1) is a member of the protein family containing a high mobility group (HMG) domain DNA-binding motif. We have functionally characterised the 71.4 kDa Zm-SSRP1 protein from maize. The chromatin-associated Zm-SSRP1 is detected by immunoblot analysis in maize leaves, kernels and suspension culture cells, but not in roots. Mediated by its HMG domain, recombinant Zm-SSRP1 interacts structure-specifically with supercoiled DNA and DNA minicircles when compared with linear DNA. In linear duplex DNA, the protein does not recognise a specific sequence, but it binds preferentially to sequences containing the deformable dinucleotide TG, as demonstrated by a random oligonucleotide selection experiment. Zm-SSRP1 modulates DNA structure by bending the target sequence, since it promotes the circularisation of short DNA fragments in the presence of DNA ligase. Moreover, Zm-SSRP1 facilitates the formation of nucleoprotein structures, as measured using the bacterial site-specific beta-mediated recombination reaction. Analysis of the subcellular localisation of various SSRP1-GFP fusions revealed that, in contrast to HMG domain transcription factors, the nuclear localisation sequence of Zm-SSRP1 is situated within a 20-amino acid residue region adjacent to the HMG domain rather than within the DNA-binding domain. The results are discussed in the context of the likely function of SSRP1 proteins in transcription and replication.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Röttgers K, Krohn NM, Lichota J, Stemmer C, Merkle T, Grasser KD. DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize. The Plant Journal. 2000;23(3):395-405.
Röttgers, K., Krohn, N. M., Lichota, J., Stemmer, C., Merkle, T., & Grasser, K. D. (2000). DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize. The Plant Journal, 23(3), 395-405.
Röttgers, K., Krohn, N. M., Lichota, J., Stemmer, C., Merkle, T., and Grasser, K. D. (2000). DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize. The Plant Journal 23, 395-405.
Röttgers, K., et al., 2000. DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize. The Plant Journal, 23(3), p 395-405.
K. Röttgers, et al., “DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize.”, The Plant Journal, vol. 23, 2000, pp. 395-405.
Röttgers, K., Krohn, N.M., Lichota, J., Stemmer, C., Merkle, T., Grasser, K.D.: DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize. The Plant Journal. 23, 395-405 (2000).
Röttgers, K., Krohn, N.M., Lichota, J., Stemmer, C., Merkle, Thomas, and Grasser, K.D. “DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize.”. The Plant Journal 23.3 (2000): 395-405.
This data publication is cited in the following publications:
This publication cites the following data publications:

12 Citations in Europe PMC

Data provided by Europe PubMed Central.

Changes in the nuclear proteome of developing wheat (Triticum aestivum L.) grain.
Bonnot T, Bancel E, Chambon C, Boudet J, Branlard G, Martre P., Front Plant Sci 6(), 2015
PMID: 26579155
Arabidopsis DEAD-box RNA helicase UAP56 interacts with both RNA and DNA as well as with mRNA export factors.
Kammel C, Thomaier M, Sorensen BB, Schubert T, Langst G, Grasser M, Grasser KD., PLoS ONE 8(3), 2013
PMID: 23555998
The plant-specific family of DNA-binding proteins containing three HMG-box domains interacts with mitotic and meiotic chromosomes.
Pedersen DS, Coppens F, Ma L, Antosch M, Marktl B, Merkle T, Beemster GT, Houben A, Grasser KD., New Phytol. 192(3), 2011
PMID: 21781122
Unexpected mobility of plant chromatin-associated HMGB proteins.
Merkle T, Grasser KD., Plant Signal Behav 6(6), 2011
PMID: 21543902
The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2.
Lolas IB, Himanen K, Gronlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD., Plant J. 61(4), 2010
PMID: 19947984
The Arabidopsis genome encodes structurally and functionally diverse HMGB-type proteins.
Grasser M, Lentz A, Lichota J, Merkle T, Grasser KD., J. Mol. Biol. 358(3), 2006
PMID: 16563436
The chromatin remodelling complex FACT associates with actively transcribed regions of the Arabidopsis genome.
Duroux M, Houben A, Ruzicka K, Friml J, Grasser KD., Plant J. 40(5), 2004
PMID: 15546350
SSRP1 functions as a co-activator of the transcriptional activator p63.
Zeng SX, Dai MS, Keller DM, Lu H., EMBO J. 21(20), 2002
PMID: 12374749

64 References

Data provided by Europe PubMed Central.

Analysis of local helix geometry in three B-DNA decamers and eight dodecamers.
Yanagi K, Prive GG, Dickerson RE., J. Mol. Biol. 217(1), 1991
PMID: 1988678
A signature for the HMG-1 box DNA-binding proteins.
Landsman D, Bustin M., Bioessays 15(8), 1993
PMID: 8135767
Phylogenetic relationships of HMG box DNA-binding domains.
Griess EA, Rensing SA, Grasser KD, Maier UG, Feix G., J. Mol. Evol. 37(2), 1993
PMID: 8411210
Curved DNA without A-A: experimental estimation of all 16 DNA wedge angles.
Bolshoy A, McNamara P, Harrington RE, Trifonov EN., Proc. Natl. Acad. Sci. U.S.A. 88(6), 1991
PMID: 2006170
A Drosophila single-strand DNA/RNA-binding factor contains a high-mobility-group box and is enriched in the nucleolus.
Hsu T, King DL, LaBonne C, Kafatos FC., Proc. Natl. Acad. Sci. U.S.A. 90(14), 1993
PMID: 7688122
Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees.
Schultz SC, Shields GC, Steitz TA., Science 253(5023), 1991
PMID: 1653449
Intercalation, DNA kinking, and the control of transcription.
Werner MH, Gronenborn AM, Clore GM., Science 271(5250), 1996
PMID: 8628992
The HMG-1 box protein family: classification and functional relationships.
Baxevanis AD, Landsman D., Nucleic Acids Res. 23(9), 1995
PMID: 7784217
A novel Arabidopsis DNA binding protein contains the conserved motif of HMG-box proteins.
Yamaguchi-Shinozaki K, Shinozaki K., Nucleic Acids Res. 20(24), 1992
PMID: 1480495
High-Mobility-Group Chromosomal Proteins: Architectural Components That Facilitate Chromatin Function
BUSTIN, 1996

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 10929132
PubMed | Europe PMC

Search this title in

Google Scholar