Import of agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins

Ziemienowicz A, Merkle T, Schoumacher F, Hohn B, Rossi L (2001)
The Plant Cell 13(2): 369-384.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
To study the mechanism of nuclear import of T-DNA, complexes consisting of the virulence proteins VirD2 and VirE2 as well as single-stranded DNA (ssDNA) were tested for import into plant nuclei in vitro. Import of these complexes was fast and efficient and could be inhibited by a competitor, a nuclear localization signal (NLS) coupled to BSA. For import of short ssDNA, VirD2 was sufficient, whereas import of long ssDNA additionally required VirE2. A VirD2 mutant lacking its C-terminal NLS was unable to mediate import of the T-DNA complexes into nuclei. Although free VirE2 molecules were imported into nuclei, once bound to ssDNA they were not imported, implying that when complexed to DNA, the NLSs of VirE2 are not exposed and thus do not function. RecA, another ssDNA binding protein, could substitute for VirE2 in the nuclear import of T-DNA but not in earlier events of T-DNA transfer to plant cells. We propose that VirD2 directs the T-DNA complex to the nuclear pore, whereas both proteins mediate its passage through the pore. Therefore, by binding to ssDNA, VirE2 may shape the T-DNA complex such that it is accepted for translocation into the nucleus.
Publishing Year
ISSN
PUB-ID

Cite this

Ziemienowicz A, Merkle T, Schoumacher F, Hohn B, Rossi L. Import of agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins. The Plant Cell. 2001;13(2):369-384.
Ziemienowicz, A., Merkle, T., Schoumacher, F., Hohn, B., & Rossi, L. (2001). Import of agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins. The Plant Cell, 13(2), 369-384.
Ziemienowicz, A., Merkle, T., Schoumacher, F., Hohn, B., and Rossi, L. (2001). Import of agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins. The Plant Cell 13, 369-384.
Ziemienowicz, A., et al., 2001. Import of agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins. The Plant Cell, 13(2), p 369-384.
A. Ziemienowicz, et al., “Import of agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins”, The Plant Cell, vol. 13, 2001, pp. 369-384.
Ziemienowicz, A., Merkle, T., Schoumacher, F., Hohn, B., Rossi, L.: Import of agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins. The Plant Cell. 13, 369-384 (2001).
Ziemienowicz, A., Merkle, Thomas, Schoumacher, F., Hohn, B., and Rossi, L. “Import of agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins”. The Plant Cell 13.2 (2001): 369-384.
This data publication is cited in the following publications:
This publication cites the following data publications:

38 Citations in Europe PMC

Data provided by Europe PubMed Central.

Agrobacterium: nature's genetic engineer.
Nester EW., Front Plant Sci 5(), 2014
PMID: 25610442
Identification of rice Di19 family reveals OsDi19-4 involved in drought resistance.
Wang L, Yu C, Chen C, He C, Zhu Y, Huang W., Plant Cell Rep. 33(12), 2014
PMID: 25236158
Visualization of VirE2 protein translocation by the Agrobacterium type IV secretion system into host cells.
Sakalis PA, van Heusden GP, Hooykaas PJ., Microbiologyopen 3(1), 2014
PMID: 24376037
Biological activity of the tzs gene of nopaline Agrobacterium tumefaciens GV3101 in plant regeneration and genetic transformation.
Han ZF, Hunter DM, Sibbald S, Zhang JS, Tian L., Mol. Plant Microbe Interact. 26(11), 2013
PMID: 24088018
Applying horizontal gene transfer phenomena to enhance non-viral gene therapy.
Elmer JJ, Christensen MD, Rege K., J Control Release 172(1), 2013
PMID: 23994344
VIP1: linking Agrobacterium-mediated transformation to plant immunity?
Liu Y, Kong X, Pan J, Li D., Plant Cell Rep. 29(8), 2010
PMID: 20473505
Transient expression of minimum linear gene cassettes in onion epidermal cells via direct transformation.
Cheng YQ, Yang J, Xu FP, An LJ, Liu JF, Chen ZW., Appl. Biochem. Biotechnol. 159(3), 2009
PMID: 19255730
Localization of the plasmid-encoded proteins TraI and MobA in eukaryotic cells.
Silby MW, Ferguson GC, Billington C, Heinemann JA., Plasmid 57(2), 2007
PMID: 17084894

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 11226191
PubMed | Europe PMC

Search this title in

Google Scholar