Phosphorylation of sucrose synthase at serine 170: occurrence and possible role as a signal for proteolysis.

Hardin SC, Tang G-Q, Scholz A, Holtgräwe D, Winter H, Huber SC (2003)
Plant J 35(5): 588-603.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ;
Abstract / Bemerkung
Sequence analysis identified serine 170 (S170) of the maize (Zea mays L.) SUS1 sucrose synthase (SUS) protein as a possible, second phosphorylation site. Maize leaves contained two calcium-dependent protein kinase activities and a calcium-independent kinase activity with characteristics of an sucrose non-fermenting 1 (SNF1)-related protein kinase. Phosphorylation of the novel S170 and the known serine 15 (S15) site by these protein kinases was determined in peptide substrates and detected in SUS1 protein substrates utilizing sequence- and phosphorylation-specific antibodies. We demonstrate phosphorylation of S170 in vitro and in vivo. The calcium-dependent protein kinases phosphorylated both S170 and S15, whereas SNF1-related protein kinase activity was restricted to S15. Calcium-dependent protein-kinase-mediated S170 and S15 phosphorylation kinetics were determined in wild-type and mutant SUS1 substrates. These analyses revealed that kinase specificity for S170 was threefold lower than that for S15, and that phosphorylation of S170 was stimulated by prior phosphorylation at the S15 site. The SUS-binding peptides encoded by early nodulin 40 (ENOD40) specifically antagonized S170 phosphorylation in vitro. A model wherein S170 phosphorylation functions as part of a mechanism targeting SUS for proteasome-mediated degradation is supported by the observations that SUS proteolytic fragments: (i) were detected and possessed relatively high phosphorylated-S170 (pS170) stoichiometry; (ii) were spatially coincident with proteasome activity within developing leaves; and (iii) co-sedimented with proteasome activity. In addition, full-length pS170-SUS protein was less stable than S170-SUS in cultured leaf segments and was stabilized by proteasome inhibition. Post-translational control of SUS protein level through pS170-promoted proteolysis may explain the specific and significant decrease in SUS abundance that accompanies the sink-to-source transition in developing maize leaves.
Erscheinungsjahr
Zeitschriftentitel
Plant J
Band
35
Zeitschriftennummer
5
Seite
588-603
ISSN
eISSN
PUB-ID

Zitieren

Hardin SC, Tang G-Q, Scholz A, Holtgräwe D, Winter H, Huber SC. Phosphorylation of sucrose synthase at serine 170: occurrence and possible role as a signal for proteolysis. Plant J. 2003;35(5):588-603.
Hardin, S. C., Tang, G. - Q., Scholz, A., Holtgräwe, D., Winter, H., & Huber, S. C. (2003). Phosphorylation of sucrose synthase at serine 170: occurrence and possible role as a signal for proteolysis. Plant J, 35(5), 588-603. doi:10.1046/j.1365-313X.2003.01831.x
Hardin, S. C., Tang, G. - Q., Scholz, A., Holtgräwe, D., Winter, H., and Huber, S. C. (2003). Phosphorylation of sucrose synthase at serine 170: occurrence and possible role as a signal for proteolysis. Plant J 35, 588-603.
Hardin, S.C., et al., 2003. Phosphorylation of sucrose synthase at serine 170: occurrence and possible role as a signal for proteolysis. Plant J, 35(5), p 588-603.
S.C. Hardin, et al., “Phosphorylation of sucrose synthase at serine 170: occurrence and possible role as a signal for proteolysis.”, Plant J, vol. 35, 2003, pp. 588-603.
Hardin, S.C., Tang, G.-Q., Scholz, A., Holtgräwe, D., Winter, H., Huber, S.C.: Phosphorylation of sucrose synthase at serine 170: occurrence and possible role as a signal for proteolysis. Plant J. 35, 588-603 (2003).
Hardin, S.C., Tang, G.-Q., Scholz, A., Holtgräwe, Daniela, Winter, H., and Huber, S.C. “Phosphorylation of sucrose synthase at serine 170: occurrence and possible role as a signal for proteolysis.”. Plant J 35.5 (2003): 588-603.

42 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Sucrose transport and carbon fluxes during wood formation.
Mahboubi A, Niittylä T., Physiol Plant 164(1), 2018
PMID: 29572842
Exploring natural genetic variation in tomato sucrose synthases on the basis of increased kinetic properties.
Dinh QD, Finkers R, Westphal AH, van Dongen WMAM, Visser RGF, Trindade LM., PLoS One 13(10), 2018
PMID: 30372500
Noncoding RNAs, Emerging Regulators in Root Endosymbioses.
Lelandais-Brière C, Moreau J, Hartmann C, Crespi M., Mol Plant Microbe Interact 29(3), 2016
PMID: 26894282
A Role for Barley Calcium-Dependent Protein Kinase CPK2a in the Response to Drought.
Cieśla A, Mituła F, Misztal L, Fedorowicz-Strońska O, Janicka S, Tajdel-Zielińska M, Marczak M, Janicki M, Ludwików A, Sadowski J., Front Plant Sci 7(), 2016
PMID: 27826303
Transgenic switchgrass (Panicum virgatum L.) biomass is increased by overexpression of switchgrass sucrose synthase (PvSUS1).
Poovaiah CR, Mazarei M, Decker SR, Turner GB, Sykes RW, Davis MF, Stewart CN., Biotechnol J 10(4), 2015
PMID: 25327983
Alterations in stem sugar content and metabolism in sorghum genotypes subjected to drought stress
Qazi HA, Srinivasa Rao P, Kashikar A, Suprasanna P, Bhargava S., Funct Plant Biol 41(9), 2014
PMID: IND600817476
Soybean miR172c targets the repressive AP2 transcription factor NNC1 to activate ENOD40 expression and regulate nodule initiation.
Wang Y, Wang L, Zou Y, Chen L, Cai Z, Zhang S, Zhao F, Tian Y, Jiang Q, Ferguson BJ, Gresshoff PM, Li X., Plant Cell 26(12), 2014
PMID: 25549672
Plastidial starch phosphorylase in sweet potato roots is proteolytically modified by protein-protein interaction with the 20S proteasome.
Lin YC, Chen HM, Chou IM, Chen AN, Chen CP, Young GH, Lin CT, Cheng CH, Chang SC, Juang RH., PLoS One 7(4), 2012
PMID: 22506077
Plant nucleotide sugar formation, interconversion, and salvage by sugar recycling.
Bar-Peled M, O'Neill MA., Annu Rev Plant Biol 62(), 2011
PMID: 21370975
Peptide signalling in the rhizobium-legume symbiosis.
Batut J, Mergaert P, Masson-Boivin C., Curr Opin Microbiol 14(2), 2011
PMID: 21236724
Dual RNAs in plants.
Bardou F, Merchan F, Ariel F, Crespi M., Biochimie 93(11), 2011
PMID: 21824505
The structure of sucrose synthase-1 from Arabidopsis thaliana and its functional implications.
Zheng Y, Anderson S, Zhang Y, Garavito RM., J Biol Chem 286(41), 2011
PMID: 21865170
Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development.
Abid G, Silue S, Muhovski Y, Jacquemin JM, Toussaint A, Baudoin JP., Gene 439(1-2), 2009
PMID: 19306919
Sugarcane genes associated with sucrose content.
Papini-Terzi FS, Rocha FR, Vêncio RZ, Felix JM, Branco DS, Waclawovsky AJ, Del Bem LE, Lembke CG, Costa MD, Nishiyama MY, Vicentini R, Vincentz MG, Ulian EC, Menossi M, Souza GM., BMC Genomics 10(), 2009
PMID: 19302712
Sucrose metabolism during fruit development in Coffea racemosa
Geromel C, Ferreira LP, Bottcher A, Pot D, Pereira LFP, Leroy T, Vieira LGE, Mazzafera P, Marraccini P., Ann Appl Biol 152(2), 2008
PMID: IND44036244
Diamonds in the rough: mRNA-like non-coding RNAs.
Rymarquis LA, Kastenmayer JP, Hüttenhofer AG, Green PJ., Trends Plant Sci 13(7), 2008
PMID: 18448381
In-depth investigation of the soybean seed-filling proteome and comparison with a parallel study of rapeseed.
Agrawal GK, Hajduch M, Graham K, Thelen JJ., Plant Physiol 148(1), 2008
PMID: 18599654
Banana (Musa spp.) as a model to study the meristem proteome: acclimation to osmotic stress.
Carpentier SC, Witters E, Laukens K, Van Onckelen H, Swennen R, Panis B., Proteomics 7(1), 2007
PMID: 17149779
Regulation of invertase: a 'suite' of transcriptional and post-transcriptional mechanisms
Huang LF, Bocock PN, Davis JM, Koch KE., Functional plant biology : FPB. 34(6), 2007
PMID: IND43941851
Light and metabolic signals control the selective degradation of sucrose synthase in maize leaves during deetiolation.
Qiu QS, Hardin SC, Mace J, Brutnell TP, Huber SC., Plant Physiol 144(1), 2007
PMID: 17400707
Peptide hormones in plants.
Matsubayashi Y, Sakagami Y., Annu Rev Plant Biol 57(), 2006
PMID: 16669777
Plant bioactive peptides: an expanding class of signaling molecules
Germain H, Chevalier E, Matton DP., Can J Bot 84(1), 2006
PMID: IND43889082
Protein phosphorylation and membrane association of sucrose synthase in developing tomato fruit.
Anguenot R, Nguyen-Quoc B, Yelle S, Michaud D., Plant Physiol Biochem 44(5-6), 2006
PMID: 16806956
The three maize sucrose synthase isoforms differ in distribution, localization, and phosphorylation.
Duncan KA, Hardin SC, Huber SC., Plant Cell Physiol 47(7), 2006
PMID: 16760218
Plants, symbiosis and parasites: a calcium signalling connection.
Harper JF, Harmon A., Nat Rev Mol Cell Biol 6(7), 2005
PMID: 16072038
Identification of a new motif for CDPK phosphorylation in vitro that suggests ACC synthase may be a CDPK substrate.
Hernández Sebastià C, Hardin SC, Clouse SD, Kieber JJ, Huber SC., Arch Biochem Biophys 428(1), 2004
PMID: 15234272
Modification of soybean sucrose synthase by S-thiolation with ENOD40 peptide A.
Röhrig H, John M, Schmidt J., Biochem Biophys Res Commun 325(3), 2004
PMID: 15541370

41 References

Daten bereitgestellt von Europe PubMed Central.

Identification of Ser-543 as the major regulatory phosphorylation site in spinach leaf nitrate reductase.
Bachmann M, Shiraishi N, Campbell WH, Yoo BC, Harmon AC, Huber SC, Davies E., Plant Cell 8(3), 1996
PMID: 8721752
Multiple, distinct isoforms of sucrose synthase in pea.
Barratt DH, Barber L, Kruger NJ, Smith AM, Wang TL, Martin C., Plant Physiol. 127(2), 2001
PMID: 11598239
Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family.
Cheng SH, Willmann MR, Chen HC, Sheen J., Plant Physiol. 129(2), 2002
PMID: 12068094
Expression of two sucrose synthase genes in endosperm and seedling cells of maize: evidence of tissue specific polymerization of protomers
Chourey, Mol. Gen. Genet. 203(), 1986
Structure and functions of the 20S and 26S proteasomes.
Coux O, Tanaka K, Goldberg AL., Annu. Rev. Biochem. 65(), 1996
PMID: 8811196
Sucrose synthase in legume nodules is essential for nitrogen fixation
Gordon AJ, Minchin FR, James CL, Komina O., Plant Physiol. 120(3), 1999
PMID: 10398723
Carbon partitioning to cellulose synthesis.
Haigler CH, Ivanova-Datcheva M, Hogan PS, Salnikov VV, Hwang S, Martin K, Delmer DP., Plant Mol. Biol. 47(1-2), 2001
PMID: 11554477
Metabolic signalling and carbon partitioning: role of Snf1-related (SnRK1) protein kinase.
Halford NG, Hey S, Jhurreea D, Laurie S, McKibbin RS, Paul M, Zhang Y., J. Exp. Bot. 54(382), 2003
PMID: 12508057
Plant development: regulation by protein degradation.
Hellmann H, Estelle M., Science 297(5582), 2002
PMID: 12161644
Phosphorylation of serine-15 of maize leaf sucrose synthase. Occurrence in vivo and possible regulatory significance.
Huber SC, Huber JL, Liao PC, Gage DA, McMichael RW Jr, Chourey PS, Hannah LC, Koch K., Plant Physiol. 112(2), 1996
PMID: 8883390
Ubiquitin- and proteasome-dependent proteolysis in plants.
Ingvardsen C, Veierskov B., Physiol Plant 112(4), 2001
PMID: 11473704
CARBOHYDRATE-MODULATED GENE EXPRESSION IN PLANTS.
Koch KE., Annu. Rev. Plant Physiol. Plant Mol. Biol. 47(), 1996
PMID: 15012299
Sugar Levels Modulate Differential Expression of Maize Sucrose Synthase Genes.
Koch KE, Nolte KD, Duke ER, McCarty DR, Avigne WT., Plant Cell 4(1), 1992
PMID: 12297629
Rice ENOD40: isolation and expression analysis in rice and transgenic soybean root nodules.
Kouchi H, Takane K, So RB, Ladha JK, Reddy PM., Plant J. 18(2), 1999
PMID: 10363365
The measurement of ubiquitin and ubiquitinated proteins.
Mimnaugh EG, Bonvini P, Neckers L., Electrophoresis 20(2), 1999
PMID: 10197449
An increase in apparent affinity for sucrose of mung bean sucrose synthase is caused by in vitro phosphorylation or directed mutagenesis of Ser11.
Nakai T, Konishi T, Zhang XQ, Chollet R, Tonouchi N, Tsuchida T, Yoshinaga F, Mori H, Sakai F, Hayashi T., Plant Cell Physiol. 39(12), 1998
PMID: 10050318
Soybean ENOD40 encodes two peptides that bind to sucrose synthase.
Rohrig H, Schmidt J, Miklashevichs E, Schell J, John M., Proc. Natl. Acad. Sci. U.S.A. 99(4), 2002
PMID: 11842184
Characteristics of 26 S proteases from fission yeast mutants, which arrest in mitosis.
Seeger M, Gordon C, Ferrell K, Dubiel W., J. Mol. Biol. 263(3), 1996
PMID: 8918598
Temporal and Spatial Expression Pattern of Sucrose Synthase during Tomato Fruit Development.
Wang F, Smith AG, Brenner ML., Plant Physiol. 104(2), 1994
PMID: 12232103
Regulation of sucrose metabolism in higher plants: localization and regulation of activity of key enzymes
Winter, Crit. Rev. Plant Sci. 19(), 2000
Identification of sucrose synthase as an actin-binding protein.
Winter H, Huber JL, Huber SC, Davies E., FEBS Lett. 430(3), 1998
PMID: 9688539
Purification and characterization of the 26S proteasome from cultured rice (Oryza sativa) cells
Yanagawa, Plant Sci. 149(), 1999
Soybean nodule sucrose synthase (nodulin-100): further analysis of its phosphorylation using recombinant and authentic root-nodule enzymes.
Zhang XQ, Lund AA, Sarath G, Cerny RL, Roberts DM, Chollet R., Arch. Biochem. Biophys. 371(1), 1999
PMID: 10525291

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 12940952
PubMed | Europe PMC

Suchen in

Google Scholar