Site preferences of insertional mutagenesis agents in Arabidopsis

Pan X, Li Y, Stein L (2005)
Plant Physiology 137(1): 168-175.

Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
; ;
Abstract / Bemerkung
We have performed a comparative analysis of the insertion sites of engineered Arabidopsis (Arabidopsis thaliana) insertional mutagenesis vectors that are based on the maize (Zea mays) transposable elements and Agrobacterium T-DNA. The transposon-based agents show marked preference for high GC content, whereas the T-DNA-based agents show preference for low GC content regions. The transposon-based agents show a bias toward insertions near the translation start codons of genes, while the T-DNAs show a predilection for the putative transcriptional regulatory regions of genes. The transposon-based agents also have higher insertion site densities in exons than do the T-DNA insertions. These observations show that the transposon-based and T-DNA-based mutagenesis techniques could complement one another well, and neither alone is sufficient to achieve the goal of saturation mutagenesis in Arabidopsis. These results also suggest that transposon-based mutagenesis techniques may prove the most effective for obtaining gene disruptions and for generating gene traps, while T-DNA-based agents may be more effective for activation tagging and enhancer trapping. From the patterns of insertion site distributions, we have identified a set of nucleotide sequence motifs that are overrepresented at the transposon insertion sites. These motifs may play a role in the transposon insertion site preferences. These results could help biologists to study the mechanisms of insertions of the insertional mutagenesis agents and to design better strategies for genome-wide insertional mutagenesis.
Plant Physiology


Pan X, Li Y, Stein L. Site preferences of insertional mutagenesis agents in Arabidopsis. Plant Physiology. 2005;137(1):168-175.
Pan, X., Li, Y., & Stein, L. (2005). Site preferences of insertional mutagenesis agents in Arabidopsis. Plant Physiology, 137(1), 168-175. doi:10.1104/pp.104.053215
Pan, X., Li, Y., and Stein, L. (2005). Site preferences of insertional mutagenesis agents in Arabidopsis. Plant Physiology 137, 168-175.
Pan, X., Li, Y., & Stein, L., 2005. Site preferences of insertional mutagenesis agents in Arabidopsis. Plant Physiology, 137(1), p 168-175.
X. Pan, Y. Li, and L. Stein, “Site preferences of insertional mutagenesis agents in Arabidopsis”, Plant Physiology, vol. 137, 2005, pp. 168-175.
Pan, X., Li, Y., Stein, L.: Site preferences of insertional mutagenesis agents in Arabidopsis. Plant Physiology. 137, 168-175 (2005).
Pan, X., Li, Yong, and Stein, L. “Site preferences of insertional mutagenesis agents in Arabidopsis”. Plant Physiology 137.1 (2005): 168-175.

23 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

A collection of enhancer trap insertional mutants for functional genomics in tomato.
Pérez-Martín F, Yuste-Lisbona FJ, Pineda B, Angarita-Díaz MP, García-Sogo B, Antón T, Sánchez S, Giménez E, Atarés A, Fernández-Lozano A, Ortíz-Atienza A, García-Alcázar M, Castañeda L, Fonseca R, Capel C, Goergen G, Sánchez J, Quispe JL, Capel J, Angosto T, Moreno V, Lozano R., Plant Biotechnol J 15(11), 2017
PMID: 28317264
The neutral metallopeptidase NMP1 of Trichoderma guizhouense is required for mycotrophy and self-defence.
Zhang J, Bayram Akcapinar G, Atanasova L, Rahimi MJ, Przylucka A, Yang D, Kubicek CP, Zhang R, Shen Q, Druzhinina IS., Environ Microbiol 18(2), 2016
PMID: 26118314
Spectrum of T-DNA integrations for insertional mutagenesis of Histoplasma capsulatum.
Kemski MM, Stevens B, Rappleye CA., Fungal Biol 117(1), 2013
PMID: 23332832
How important are transposons for plant evolution?
Lisch D., Nat Rev Genet 14(1), 2013
PMID: 23247435
Analysis of selected singleton transposable elements (SSTEs) and their application for the development of land PATE markers in Magnaporthe oryzae
Zhang Hy, He Dy, Kasetsomboon T, Zhou H, Li P, Li Xl, Jantasuriyarat C, Zhou B., J. Gen. Plant Pathol. 79(2), 2013
PMID: IND500635846
A versatile gene trap to visualize and interrogate the function of the vertebrate proteome.
Trinh le A, Hochgreb T, Graham M, Wu D, Ruf-Zamojski F, Jayasena CS, Saxena A, Hawk R, Gonzalez-Serricchio A, Dixson A, Chow E, Gonzales C, Leung HY, Solomon I, Bronner-Fraser M, Megason SG, Fraser SE., Genes Dev 25(21), 2011
PMID: 22056673
SNP discovery and genetic mapping of T-DNA insertional mutants in Fragaria vesca L.
Ruiz-Rojas JJ, Sargent DJ, Shulaev V, Dickerman AW, Pattison J, Holt SH, Ciordia A, Veilleux RE., Theor Appl Genet 121(3), 2010
PMID: 20349033
Epigenetic regulation of transposable elements in plants.
Lisch D., Annu Rev Plant Biol 60(), 2009
PMID: 19007329
The Lmgpi15 gene, encoding a component of the glycosylphosphatidylinositol anchor biosynthesis pathway, is required for morphogenesis and pathogenicity in Leptosphaeria maculans.
Remy E, Meyer M, Blaise F, Simon UK, Kuhn D, Chabirand M, Riquelme M, Balesdent MH, Rouxel T., New Phytol 179(4), 2008
PMID: 18557818
Many or most genes in Arabidopsis transposed after the origin of the order Brassicales.
Freeling M, Lyons E, Pedersen B, Alam M, Ming R, Lisch D., Genome Res 18(12), 2008
PMID: 18836034
An efficient method for producing an indexed, insertional-mutant library in rice.
He C, Dey M, Lin Z, Duan F, Li F, Wu R., Genomics 89(4), 2007
PMID: 17229544
Discovery of chemically induced mutations in rice by TILLING.
Till BJ, Cooper J, Tai TH, Colowit P, Greene EA, Henikoff S, Comai L., BMC Plant Biol 7(), 2007
PMID: 17428339
Genetic transformation in potato: Approaches and strategies.
Chakravarty B, Wang-Pruski G, Flinn B, Gustafson V, Regan S., Am. J. Potato Res. 84(4), 2007
PMID: IND43959874
Genome-wide analysis of T-DNA integration into the chromosomes of Magnaporthe oryzae.
Choi J, Park J, Jeon J, Chi MH, Goh J, Yoo SY, Park J, Jung K, Kim H, Park SY, Rho HS, Kim S, Kim BR, Han SS, Kang S, Lee YH., Mol Microbiol 66(2), 2007
PMID: 17850257
A systematic analysis of T-DNA insertion events in Magnaporthe oryzae.
Meng Y, Patel G, Heist M, Betts MF, Tucker SL, Galadima N, Donofrio NM, Brown D, Mitchell TK, Li L, Xu JR, Orbach M, Thon M, Dean RA, Farman ML., Fungal Genet Biol 44(10), 2007
PMID: 17544743
Diverse developmental mutants revealed in an activation-tagged population of poplar
Harrison EJ, Bush M, Plett JM, McPhee DP, Vitez R, O'Malley B, Sharma V, Bosnich W, Séguin A, MacKay J., Can J Bot 85(11), 2007
PMID: IND44007647
Mapped Ds/T-DNA launch pads for functional genomics in barley.
Zhao T, Palotta M, Langridge P, Prasad M, Graner A, Schulze-Lefert P, Koprek T., Plant J 47(5), 2006
PMID: 16889649
An alpha-(1,4)-amylase is essential for alpha-(1,3)-glucan production and virulence in Histoplasma capsulatum.
Marion CL, Rappleye CA, Engle JT, Goldman WE., Mol Microbiol 62(4), 2006
PMID: 17038119
Novel gene functions required for melanization of the human pathogen Cryptococcus neoformans.
Walton FJ, Idnurm A, Heitman J., Mol Microbiol 57(5), 2005
PMID: 16102007


Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®


PMID: 15618417
PubMed | Europe PMC

Suchen in

Google Scholar