RHM2 is involved in mucilage pectin synthesis and is required for the development of the seed coat in Arabidopsis

Usadel B, Kuschinsky AM, Rosso MG, Eckermann N, Pauly M (2004)
Plant Physiology 134(1): 286-295.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Pectins are major components of primary plant cell walls and the seed mucilage of Arabidopsis. Despite progress in the structural elucidation of pectins, only very few enzymes participating in or regulating their synthesis have been identified. A first candidate gene involved in the synthesis of pectinaceous rhamnogalacturonan I is RHM2, a putative plant ortholog to NDP-rhamnose biosynthetic enzymes in bacteria. Expression studies with a promoter beta-glucuronidase construct and reverse transcription PCR data show that RHM2 is expressed ubiquitously. Rhm2 T-DNA insertion mutant lines were identified using a reverse genetics approach. Analysis of the rhm2 seeds by various staining methods and chemical analysis of the mucilage revealed a strong reduction of rhamnogalacturonan I in the mucilage and a decrease of its molecular weight. In addition, scanning electron microscopy of the seed surface indicated a distorted testa morphology, illustrating not only a structural but also a developmental role for RGI or rhamnose metabolism in proper testa formation.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Usadel B, Kuschinsky AM, Rosso MG, Eckermann N, Pauly M. RHM2 is involved in mucilage pectin synthesis and is required for the development of the seed coat in Arabidopsis. Plant Physiology. 2004;134(1):286-295.
Usadel, B., Kuschinsky, A. M., Rosso, M. G., Eckermann, N., & Pauly, M. (2004). RHM2 is involved in mucilage pectin synthesis and is required for the development of the seed coat in Arabidopsis. Plant Physiology, 134(1), 286-295.
Usadel, B., Kuschinsky, A. M., Rosso, M. G., Eckermann, N., and Pauly, M. (2004). RHM2 is involved in mucilage pectin synthesis and is required for the development of the seed coat in Arabidopsis. Plant Physiology 134, 286-295.
Usadel, B., et al., 2004. RHM2 is involved in mucilage pectin synthesis and is required for the development of the seed coat in Arabidopsis. Plant Physiology, 134(1), p 286-295.
B. Usadel, et al., “RHM2 is involved in mucilage pectin synthesis and is required for the development of the seed coat in Arabidopsis”, Plant Physiology, vol. 134, 2004, pp. 286-295.
Usadel, B., Kuschinsky, A.M., Rosso, M.G., Eckermann, N., Pauly, M.: RHM2 is involved in mucilage pectin synthesis and is required for the development of the seed coat in Arabidopsis. Plant Physiology. 134, 286-295 (2004).
Usadel, B., Kuschinsky, A.M., Rosso, Mario G., Eckermann, N., and Pauly, M. “RHM2 is involved in mucilage pectin synthesis and is required for the development of the seed coat in Arabidopsis”. Plant Physiology 134.1 (2004): 286-295.
This data publication is cited in the following publications:
This publication cites the following data publications:

41 Citations in Europe PMC

Data provided by Europe PubMed Central.

Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
Huang D, Wang C, Yuan J, Cao J, Lan H., Acta Biochim. Biophys. Sin. (Shanghai) 47(10), 2015
PMID: 26341978
Arabidopsis seed mucilage secretory cells: regulation and dynamics.
Francoz E, Ranocha P, Burlat V, Dunand C., Trends Plant Sci. 20(8), 2015
PMID: 25998090
Starting to gel: how Arabidopsis seed coat epidermal cells produce specialized secondary cell walls.
Voiniciuc C, Yang B, Schmidt MH, Gunl M, Usadel B., Int J Mol Sci 16(2), 2015
PMID: 25658798
Local evolution of seed flotation in Arabidopsis.
Saez-Aguayo S, Rondeau-Mouro C, Macquet A, Kronholm I, Ralet MC, Berger A, Salle C, Poulain D, Granier F, Botran L, Loudet O, de Meaux J, Marion-Poll A, North HM., PLoS Genet. 10(3), 2014
PMID: 24625826
TRANSPARENT TESTA2 regulates embryonic fatty acid biosynthesis by targeting FUSCA3 during the early developmental stage of Arabidopsis seeds.
Wang Z, Chen M, Chen T, Xuan L, Li Z, Du X, Zhou L, Zhang G, Jiang L., Plant J. 77(5), 2014
PMID: 24397827
Diverse roles of Groucho/Tup1 co-repressors in plant growth and development.
Lee JE, Golz JF., Plant Signal Behav 7(1), 2012
PMID: 22301974
Increase in pectin deposition by overexpression of an ERF gene in cultured cells of Arabidopsis thaliana.
Nakano T, Naito Y, Kakegawa K, Ohtsuki N, Tsujimoto-Inui Y, Shinshi H, Suzuki K., Biotechnol. Lett. 34(4), 2012
PMID: 22160296
Degradation of seed mucilage by soil microflora promotes early seedling growth of a desert sand dune plant.
Yang X, Baskin CC, Baskin JM, Zhang W, Huang Z., Plant Cell Environ. 35(5), 2012
PMID: 22070663
Evolution of plant nucleotide-sugar interconversion enzymes.
Yin Y, Huang J, Gu X, Bar-Peled M, Xu Y., PLoS ONE 6(11), 2011
PMID: 22125650
Mass spectrometry for pectin structure analysis.
Ralet MC, Lerouge P, Quemener B., Carbohydr. Res. 344(14), 2009
PMID: 19058795

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 14671019
PubMed | Europe PMC

Search this title in

Google Scholar