Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins

Akrap N, Seidel T, Barisas BG (2010)
ANALYTICAL BIOCHEMISTRY 402(1): 105-106.

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We present, for the red fluorescent protein mCherry acting as both fluorescence resonant energy transfer (FRET) donor and acceptor, Forster critical distance (r(0)) values with five important visible fluorescent protein (VFP) variants as well as with itself. The pair EYFP-mCherry exhibits an r(0) of 5.66 nm, equaling or exceeding any combination of VFPs reported previously. Moreover, mCherry should be an excellent chromophore for homo-FRET with an r(0) of 5.10 nm for energy transfer between two mCherry moieties. Finally, mCherry exhibits higher r(0) values than does DsRed. These characteristics, combined with mCherry's rapid folding and excellent spectral properties, suggest that mCherry constitutes a valuable long-wavelength hetero-FRET acceptor and probe for homo-FRET experiments. (C) 2010 Elsevier Inc. All rights reserved.
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Akrap N, Seidel T, Barisas BG. Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. ANALYTICAL BIOCHEMISTRY. 2010;402(1):105-106.
Akrap, N., Seidel, T., & Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. ANALYTICAL BIOCHEMISTRY, 402(1), 105-106. doi:10.1016/j.ab.2010.03.026
Akrap, N., Seidel, T., and Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. ANALYTICAL BIOCHEMISTRY 402, 105-106.
Akrap, N., Seidel, T., & Barisas, B.G., 2010. Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. ANALYTICAL BIOCHEMISTRY, 402(1), p 105-106.
N. Akrap, T. Seidel, and B.G. Barisas, “Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins”, ANALYTICAL BIOCHEMISTRY, vol. 402, 2010, pp. 105-106.
Akrap, N., Seidel, T., Barisas, B.G.: Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. ANALYTICAL BIOCHEMISTRY. 402, 105-106 (2010).
Akrap, Nina, Seidel, Thorsten, and Barisas, B. George. “Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins”. ANALYTICAL BIOCHEMISTRY 402.1 (2010): 105-106.
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