Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3

Smits THM, Rezzonico F, Pelludat C, Goesmann A, Frey JE, Duffy B (2010)
FEMS MICROBIOLOGY LETTERS 308(1): 48-54.

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Abstract
A 530-kb megaplasmid pPag3 contributing 10.8% of the total genome of Pantoea vagans biocontrol strain C9-1 was sequenced. A rare nonpigmented variant C9-1W was obtained and shown to have lost pPag3, but retained all other plasmids (pPag1, pPag2). Phenotypic characterization of the variant confirmed the function of several annotated genes that may influence ecological fitness and efficacy. Metabolic profiling revealed important plasmid-based carbon utilization phenotypes. Plasmid loss resulted in thiamine auxotrophy, absence of carotenoid pigmentation, desferrioxamine diffusible siderophore biosynthesis, inherent ampicillin resistance and expression of AI-1 quorum-sensing signaling. This confirmed the functional expression of the corresponding genes located on pPag3 in P. vagans.
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Smits THM, Rezzonico F, Pelludat C, Goesmann A, Frey JE, Duffy B. Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS MICROBIOLOGY LETTERS. 2010;308(1):48-54.
Smits, T. H. M., Rezzonico, F., Pelludat, C., Goesmann, A., Frey, J. E., & Duffy, B. (2010). Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS MICROBIOLOGY LETTERS, 308(1), 48-54.
Smits, T. H. M., Rezzonico, F., Pelludat, C., Goesmann, A., Frey, J. E., and Duffy, B. (2010). Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS MICROBIOLOGY LETTERS 308, 48-54.
Smits, T.H.M., et al., 2010. Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS MICROBIOLOGY LETTERS, 308(1), p 48-54.
T.H.M. Smits, et al., “Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3”, FEMS MICROBIOLOGY LETTERS, vol. 308, 2010, pp. 48-54.
Smits, T.H.M., Rezzonico, F., Pelludat, C., Goesmann, A., Frey, J.E., Duffy, B.: Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS MICROBIOLOGY LETTERS. 308, 48-54 (2010).
Smits, Theo H. M., Rezzonico, Fabio, Pelludat, Cosima, Goesmann, Alexander, Frey, Juerg E., and Duffy, Brion. “Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3”. FEMS MICROBIOLOGY LETTERS 308.1 (2010): 48-54.
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Draft genome sequences of Pantoea agglomerans and Pantoea vagans isolates associated with termites.
Palmer M, de Maayer P, Poulsen M, Steenkamp ET, van Zyl E, Coutinho TA, Venter SN., Stand Genomic Sci 11(), 2016
PMID: 26937267
Comparative genome analysis of Enterobacter cloacae.
Liu WY, Wong CF, Chung KM, Jiang JW, Leung FC., PLoS ONE 8(9), 2013
PMID: 24069314
The large universal Pantoea plasmid LPP-1 plays a major role in biological and ecological diversification.
De Maayer P, Chan WY, Blom J, Venter SN, Duffy B, Smits TH, Coutinho TA., BMC Genomics 13(), 2012
PMID: 23151240
Fire blight: applied genomic insights of the pathogen and host.
Malnoy M, Martens S, Norelli JL, Barny MA, Sundin GW, Smits TH, Duffy B., Annu Rev Phytopathol 50(), 2012
PMID: 22702352
Characterization of the biosynthetic operon for the antibacterial peptide herbicolin in Pantoea vagans biocontrol strain C9-1 and incidence in Pantoea species.
Kamber T, Lansdell TA, Stockwell VO, Ishimaru CA, Smits TH, Duffy B., Appl. Environ. Microbiol. 78(12), 2012
PMID: 22504810
The complete genome sequence of Pantoea ananatis AJ13355, an organism with great biotechnological potential.
Hara Y, Kadotani N, Izui H, Katashkina JI, Kuvaeva TM, Andreeva IG, Golubeva LI, Malko DB, Makeev VJ, Mashko SV, Kozlov YI., Appl. Microbiol. Biotechnol. 93(1), 2012
PMID: 22159605
Metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1.
Smits TH, Rezzonico F, Kamber T, Blom J, Goesmann A, Ishimaru CA, Frey JE, Stockwell VO, Duffy B., PLoS ONE 6(7), 2011
PMID: 21789243
Antibiosis activity of Pantoea agglomerans biocontrol strain E325 against Erwinia amylovora on apple flower stigmas.
Pusey PL, Stockwell VO, Reardon CL, Smits TH, Duffy B., Phytopathology 101(10), 2011
PMID: 21679036

47 References

Data provided by Europe PubMed Central.

Multiple antibiotic production by Erwinia herbicola.
Ishimaru CA, Klos EJ, Brubaker RR., Phytopathology 78(6), 1988
PMID: IND88033164
Management of fire blight
Johnson, a case study in microbial ecology 36(), 1998
Effect of sdiA on biosensors of N-acylhomoserine lactones.
Lindsay A, Ahmer BM., J. Bacteriol. 187(14), 2005
PMID: 15995228
In situ activation of the quorum-sensing transcription factor TraR by cognate and noncognate acyl-homoserine lactone ligands
Luo, kinetics and consequences 185(), 2003
Pantoea agglomerans pvs. gypsophilae and betae, recently evolved pathogens?
Manulis S, Barash I., Mol. Plant Pathol. 4(5), 2003
PMID: IND43614990
GenDB--an open source genome annotation system for prokaryote genomes.
Meyer F, Goesmann A, McHardy AC, Bartels D, Bekel T, Clausen J, Kalinowski J, Linke B, Rupp O, Giegerich R, Puhler A., Nucleic Acids Res. 31(8), 2003
PMID: 12682369
Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli.
Misawa N, Nakagawa M, Kobayashi K, Yamano S, Izawa Y, Nakamura K, Harashima K., J. Bacteriol. 172(12), 1990
PMID: 2254247
Genetic organization of the hrp gene cluster and dspAE/BF operon in Erwinia herbicola pv. gypsophilae.
Mor H, Manulis S, Zuck M, Nizan R, Coplin DL, Barash I., Mol. Plant Microbe Interact. 14(3), 2001
PMID: 11277443
The plant pathogen Pantoea ananatis produces N-acylhomoserine lactone and causes center rot disease of onion by quorum sensing.
Morohoshi T, Nakamura Y, Yamazaki G, Ishida A, Kato N, Ikeda T., J. Bacteriol. 189(22), 2007
PMID: 17827290
Molecular organization of the Escherichia coli gab cluster
Niegemann, nucleotide sequence of the structural genes gabD and gabP and expression of the GABA permease gene 160(), 1993
The type III effectors HsvG and HsvB of gall-forming Pantoea agglomerans determine host specificity and function as transcriptional activators.
Nissan G, Manulis-Sasson S, Weinthal D, Mor H, Sessa G, Barash I., Mol. Microbiol. 61(5), 2006
PMID: 16879413
Genotypic comparison of Pantoea agglomerans plant and clinical strains.
Rezzonico F, Smits TH, Montesinos E, Frey JE, Duffy B., BMC Microbiol. 9(), 2009
PMID: 19772624

Sambrook, 1989
Identification of carotenoids in Erwinia herbicola and in a transformed Escherichia coli strain
Sandmann, FEMS Microbiol Lett 71(), 1990
Universal chemical assay for the detection and determination of siderophores.
Schwyn B, Neilands JB., Anal. Biochem. 160(1), 1987
PMID: 2952030
Structural biology of enzymes of the thiamin biosynthesis pathway
Settembre, Curr Opin Struc Biol 13(), 2003
Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography
Shaw, P Natl Acad Sci USA 94(), 1997
Complete genome sequencing of Pantoea agglomerans strain C9-1
Smits, IOBC Bull 43(), 2009
Bioavailability of iron to Pseudomonas fluorescens strain A506 on flowers of pear and apple
Temple TN, Stockwell VO, Loper JE, Johnson KB., Phytopathology 94(12), 2004
PMID: IND43659493
Quorum sensing in plant-pathogenic bacteria.
Von Bodman SB, Bauer WD, Coplin DL., Annu Rev Phytopathol 41(), 2003
PMID: 12730390

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