Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins

Lewis C, Jockusch H, Ohlendieck K (2010)
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY 2010: 1-20.

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Abstract
Although Duchenne muscular dystrophy is primarily classified as a neuromuscular disease, cardiac complications play an important role in the course of this X-linked inherited disorder. The pathobiochemical steps causing a progressive decline in the dystrophic heart are not well understood. We therefore carried out a fluorescence difference in-gel electrophoretic analysis of 9-month-old dystrophin-deficient versus age-matched normal heart, using the established MDX mouse model of muscular dystrophy-related cardiomyopathy. Out of 2,509 detectable protein spots, 79 2D-spots showed a drastic differential expression pattern, with the concentration of 3 proteins being increased, including nucleoside diphosphate kinase and lamin-A/C, and of 26 protein species being decreased, including ATP synthase, fatty acid binding-protein, isocitrate dehydrogenase, NADH dehydrogenase, porin, peroxiredoxin, adenylate kinase, tropomyosin, actin, and myosin light chains. Hence, the lack of cardiac dystrophin appears to trigger a generally perturbed protein expression pattern in the MDX heart, affecting especially energy metabolism and contractile proteins.
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Lewis C, Jockusch H, Ohlendieck K. Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY. 2010;2010:1-20.
Lewis, C., Jockusch, H., & Ohlendieck, K. (2010). Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2010, 1-20.
Lewis, C., Jockusch, H., and Ohlendieck, K. (2010). Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY 2010, 1-20.
Lewis, C., Jockusch, H., & Ohlendieck, K., 2010. Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2010, p 1-20.
C. Lewis, H. Jockusch, and K. Ohlendieck, “Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins”, JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, vol. 2010, 2010, pp. 1-20.
Lewis, C., Jockusch, H., Ohlendieck, K.: Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY. 2010, 1-20 (2010).
Lewis, Caroline, Jockusch, Harald, and Ohlendieck, Kay. “Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins”. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY 2010 (2010): 1-20.
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