Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy

Göhler A, Andre S, Kaltner H, Sauer M, Gabius H-J, Doose S (2010)
BIOPHYSICAL JOURNAL 98(12): 3044-3053.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Fluorescence correlation spectroscopy is applied on homologous human lectins (i.e., adhesion/growth-regulatory galectins) to detect influence of ligand binding and presence of the linker peptide in tandem-repeat-type proteins on hydrodynamic properties. Among five tested proteins, lactose binding increased the diffusion constant only in the cases of homodimeric galectin-1 and the linkerless variant of tandem-repeat-type galectin-4. To our knowledge, the close structural similarity among galectins does not translate into identical response to ligand binding. Kinetic measurements show association and dissociation rate constants in the order of 1 to 10(3) M-1 s(-1) and 10(-4) s(-1), respectively. Presence of the linker peptide in tandem-repeat-type protein leads to anomalous scaling with molecular mass. These results provide what we believe to be new insights into lectin responses to glycan binding, detectable so far only by small angle neutron scattering, and the structural relevance of the linker peptide. Methodologically, fluorescence correlation spectroscopy is shown to be a rather simple technical tool to characterize hydrodynamic properties of these proteins at a high level of sensitivity.
Erscheinungsjahr
Zeitschriftentitel
BIOPHYSICAL JOURNAL
Band
98
Zeitschriftennummer
12
Seite
3044-3053
ISSN
PUB-ID

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Göhler A, Andre S, Kaltner H, Sauer M, Gabius H-J, Doose S. Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy. BIOPHYSICAL JOURNAL. 2010;98(12):3044-3053.
Göhler, A., Andre, S., Kaltner, H., Sauer, M., Gabius, H. - J., & Doose, S. (2010). Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy. BIOPHYSICAL JOURNAL, 98(12), 3044-3053. doi:10.1016/j.bpj.2010.03.040
Göhler, A., Andre, S., Kaltner, H., Sauer, M., Gabius, H. - J., and Doose, S. (2010). Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy. BIOPHYSICAL JOURNAL 98, 3044-3053.
Göhler, A., et al., 2010. Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy. BIOPHYSICAL JOURNAL, 98(12), p 3044-3053.
A. Göhler, et al., “Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy”, BIOPHYSICAL JOURNAL, vol. 98, 2010, pp. 3044-3053.
Göhler, A., Andre, S., Kaltner, H., Sauer, M., Gabius, H.-J., Doose, S.: Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy. BIOPHYSICAL JOURNAL. 98, 3044-3053 (2010).
Göhler, Antonia, Andre, Sabine, Kaltner, Herbert, Sauer, Markus, Gabius, Hans-Joachim, and Doose, Soeren. “Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy”. BIOPHYSICAL JOURNAL 98.12 (2010): 3044-3053.

25 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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Ruiz FM, Gilles U, Lindner I, André S, Romero A, Reusch D, Gabius HJ., Chemistry 21(39), 2015
PMID: 26270612
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Kübler D, Seidler J, André S, Kumar S, Schwartz-Albiez R, Lehmann WD, Gabius HJ., Anal Biochem 449(), 2014
PMID: 24333252
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Ruiz FM, Scholz BA, Buzamet E, Kopitz J, André S, Menéndez M, Romero A, Solís D, Gabius HJ., FEBS J 281(5), 2014
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Ruiz FM, Fernández IS, López-Merino L, Lagartera L, Kaltner H, Menéndez M, André S, Solís D, Gabius HJ, Romero A., Acta Crystallogr D Biol Crystallogr 69(pt 9), 2013
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Stancic M, Slijepcevic D, Nomden A, Vos MJ, de Jonge JC, Sikkema AH, Gabius HJ, Hoekstra D, Baron W., Glia 60(6), 2012
PMID: 22431161
Adhesion/growth-regulatory galectins in the human eye: localization profiles and tissue reactivities as a standard to detect disease-associated alterations.
Schlötzer-Schrehardt U, André S, Janko C, Kaltner H, Kopitz J, Gabius HJ, Herrmann M., Graefes Arch Clin Exp Ophthalmol 250(8), 2012
PMID: 22527325
Comparative study of the glycan specificities of cell-bound human tandem-repeat-type galectin-4, -8 and -9.
Vokhmyanina OA, Rapoport EM, André S, Severov VV, Ryzhov I, Pazynina GV, Korchagina E, Gabius HJ, Bovin NV., Glycobiology 22(9), 2012
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Inhibitory potential of chemical substitutions at bioinspired sites of β-D-galactopyranose on neoglycoprotein/cell surface binding of two classes of medically relevant lectins.
Giguère D, André S, Bonin MA, Bellefleur MA, Provencal A, Cloutier P, Pucci B, Roy R, Gabius HJ., Bioorg Med Chem 19(10), 2011
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Witten KG, Rech C, Eckert T, Charrak S, Richtering W, Elling L, Simon U., Small 7(14), 2011
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Sensing ligand binding to a clinically relevant lectin by tryptophan fluorescence anisotropy.
Göhler A, Büchner C, André S, Doose S, Kaltner H, Gabius HJ., Analyst 136(24), 2011
PMID: 22016878

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