Live-cell super-resolution imaging with trimethoprim conjugates

Wombacher R, Heidbreder M, van de Linde S, Sheetz MP, Heilemann M, Cornish VW, Sauer M (2010)
NATURE METHODS 7(9): 717-719.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at similar to 20 nm resolution.


Wombacher R, Heidbreder M, van de Linde S, et al. Live-cell super-resolution imaging with trimethoprim conjugates. NATURE METHODS. 2010;7(9):717-719.
Wombacher, R., Heidbreder, M., van de Linde, S., Sheetz, M. P., Heilemann, M., Cornish, V. W., & Sauer, M. (2010). Live-cell super-resolution imaging with trimethoprim conjugates. NATURE METHODS, 7(9), 717-719. doi:10.1038/NMETH.1489
Wombacher, R., Heidbreder, M., van de Linde, S., Sheetz, M. P., Heilemann, M., Cornish, V. W., and Sauer, M. (2010). Live-cell super-resolution imaging with trimethoprim conjugates. NATURE METHODS 7, 717-719.
Wombacher, R., et al., 2010. Live-cell super-resolution imaging with trimethoprim conjugates. NATURE METHODS, 7(9), p 717-719.
R. Wombacher, et al., “Live-cell super-resolution imaging with trimethoprim conjugates”, NATURE METHODS, vol. 7, 2010, pp. 717-719.
Wombacher, R., Heidbreder, M., van de Linde, S., Sheetz, M.P., Heilemann, M., Cornish, V.W., Sauer, M.: Live-cell super-resolution imaging with trimethoprim conjugates. NATURE METHODS. 7, 717-719 (2010).
Wombacher, Richard, Heidbreder, Meike, van de Linde, Sebastian, Sheetz, Michael P., Heilemann, Mike, Cornish, Virginia W., and Sauer, Markus. “Live-cell super-resolution imaging with trimethoprim conjugates”. NATURE METHODS 7.9 (2010): 717-719.

148 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Coordinate-based colocalization analysis of single-molecule localization microscopy data.
Malkusch S, Endesfelder U, Mondry J, Gelléri M, Verveer PJ, Heilemann M., Histochem Cell Biol 137(1), 2012
PMID: 22086768
Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging.
van de Linde S, Krstić I, Prisner T, Doose S, Heilemann M, Sauer M., Photochem Photobiol Sci 10(4), 2011
PMID: 21152594
FRET in cell biology: still shining in the age of super-resolution?
Grecco HE, Verveer PJ., Chemphyschem 12(3), 2011
PMID: 21344589
Live-cell dSTORM with SNAP-tag fusion proteins.
Klein T, Löschberger A, Proppert S, Wolter S, van de Linde S, Sauer M., Nat Methods 8(1), 2011
PMID: 21191367
Nanoscopy of cell architecture: The actin-membrane interface.
Ahmed S., Bioarchitecture 1(1), 2011
PMID: 21866260
Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe.
Izeddin I, Specht CG, Lelek M, Darzacq X, Triller A, Zimmer C, Dahan M., PLoS One 6(1), 2011
PMID: 21264214
PALM and STORM: unlocking live-cell super-resolution.
Henriques R, Griffiths C, Hesper Rego E, Mhlanga MM., Biopolymers 95(5), 2011
PMID: 21254001
Comparison between SOFI and STORM.
Geissbuehler S, Dellagiacoma C, Lasser T., Biomed Opt Express 2(3), 2011
PMID: 21412447
Histochemistry: live and in color.
Lavis LD., J Histochem Cytochem 59(2), 2011
PMID: 21339179
Principles of chromosomal organization: lessons from yeast.
Zimmer C, Fabre E., J Cell Biol 192(5), 2011
PMID: 21383075
Measuring localization performance of super-resolution algorithms on very active samples.
Wolter S, Endesfelder U, van de Linde S, Heilemann M, Sauer M., Opt Express 19(8), 2011
PMID: 21503016
Fast, three-dimensional super-resolution imaging of live cells.
Jones SA, Shim SH, He J, Zhuang X., Nat Methods 8(6), 2011
PMID: 21552254
Chemical tags: applications in live cell fluorescence imaging.
Wombacher R, Cornish VW., J Biophotonics 4(6), 2011
PMID: 21567974
Cross-linking of DNA through HMGA1 suggests a DNA scaffold.
Vogel B, Löschberger A, Sauer M, Hock R., Nucleic Acids Res 39(16), 2011
PMID: 21596776
Kinetic studies on visible-light-switchable photochromic fluorophores based on diarylethenes.
Seefeldt B, Altenhöner K, Tosic O, Geisler T, Sauer M, Mattay J., Photochem Photobiol Sci 10(9), 2011
PMID: 21603721
Direct stochastic optical reconstruction microscopy with standard fluorescent probes.
van de Linde S, Löschberger A, Klein T, Heidbreder M, Wolter S, Heilemann M, Sauer M., Nat Protoc 6(7), 2011
PMID: 21720313
Chemical tags for labeling proteins inside living cells.
Jing C, Cornish VW., Acc Chem Res 44(9), 2011
PMID: 21879706
Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis.
Sengupta P, Jovanovic-Talisman T, Skoko D, Renz M, Veatch SL, Lippincott-Schwartz J., Nat Methods 8(11), 2011
PMID: 21926998
Super-resolution 3D microscopy of live whole cells using structured illumination.
Shao L, Kner P, Rego EH, Gustafsson MG., Nat Methods 8(12), 2011
PMID: 22002026
Assessing the localization of centrosomal proteins by PALM/STORM nanoscopy.
Sillibourne JE, Specht CG, Izeddin I, Hurbain I, Tran P, Triller A, Darzacq X, Dahan M, Bornens M., Cytoskeleton (Hoboken) 68(11), 2011
PMID: 21976302
Breaking the diffraction barrier: super-resolution imaging of cells.
Huang B, Babcock H, Zhuang X., Cell 143(7), 2010
PMID: 21168201

25 References

Daten bereitgestellt von Europe PubMed Central.

shannon, proceedings of the institute of radio engineers 37(), 1949
Chromosome dynamics in the yeast interphase nucleus.
Heun P, Laroche T, Shimada K, Furrer P, Gasser SM., Science 294(5549), 2001
PMID: 11739961
Subdiffraction-resolution fluorescence microscopy of myosin-actin motility.
Endesfelder U, van de Linde S, Wolter S, Sauer M, Heilemann M., Chemphyschem 11(4), 2010
PMID: 20186905
Selective chemical labeling of proteins in living cells.
Miller LW, Cornish VW., Curr Opin Chem Biol 9(1), 2005
PMID: 15701454
Stimulated emission depletion nanoscopy of living cells using SNAP-tag fusion proteins.
Hein B, Willig KI, Wurm CA, Westphal V, Jakobs S, Hell SW., Biophys. J. 98(1), 2010
PMID: 20074516
In vivo protein labeling with trimethoprim conjugates: a flexible chemical tag.
Miller LW, Cai Y, Sheetz MP, Cornish VW., Nat. Methods 2(4), 2005
PMID: 15782216
A chromatin folding model that incorporates linker variability generates fibers resembling the native structures.
Woodcock CL, Grigoryev SA, Horowitz RA, Whitaker N., Proc. Natl. Acad. Sci. U.S.A. 90(19), 1993
PMID: 8415647
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
Rust MJ, Bates M, Zhuang X., Nat. Methods 3(10), 2006
PMID: 16896339
Imaging intracellular fluorescent proteins at nanometer resolution.
Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF., Science 313(5793), 2006
PMID: 16902090
Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.
Hess ST, Girirajan TP, Mason MD., Biophys. J. 91(11), 2006
PMID: 16980368
Optimized fluorescent trimethoprim derivatives for in vivo protein labeling.
Calloway NT, Choob M, Sanz A, Sheetz MP, Miller LW, Cornish VW., Chembiochem 8(7), 2007
PMID: 17378009
Far-field optical nanoscopy.
Hell SW., Science 316(5828), 2007
PMID: 17525330
Highly inclined thin illumination enables clear single-molecule imaging in cells.
Tokunaga M, Imamoto N, Sakata-Sogawa K., Nat. Methods 5(2), 2008
PMID: 18176568
High-density mapping of single-molecule trajectories with photoactivated localization microscopy.
Manley S, Gillette JM, Patterson GH, Shroff H, Hess HF, Betzig E, Lippincott-Schwartz J., Nat. Methods 5(2), 2008
PMID: 18193054
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics.
Shroff H, Galbraith CG, Galbraith JA, Betzig E., Nat. Methods 5(5), 2008
PMID: 18408726
Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes
Heilemann, Angewandte Chemie International Edition 47(33), 2008
Fluorescence nanoscopy by ground-state depletion and single-molecule return.
Folling J, Bossi M, Bock H, Medda R, Wurm CA, Hein B, Jakobs S, Eggeling C, Hell SW., Nat. Methods 5(11), 2008
PMID: 18794861
Precise nanometer localization analysis for individual fluorescent probes.
Thompson RE, Larson DR, Webb WW., Biophys. J. 82(5), 2002
PMID: 11964263
Multicolor photoswitching microscopy for subdiffraction-resolution fluorescence imaging.
van de Linde S, Endesfelder U, Mukherjee A, Schuttpelz M, Wiebusch G, Wolter S, Heilemann M, Sauer M., Photochem. Photobiol. Sci. 8(4), 2009
PMID: 19337659
Super-resolution video microscopy of live cells by structured illumination.
Kner P, Chhun BB, Griffis ER, Winoto L, Gustafsson MG., Nat. Methods 6(5), 2009
PMID: 19404253
An in vivo covalent TMP-tag based on proximity-induced reactivity.
Gallagher SS, Sable JE, Sheetz MP, Cornish VW., ACS Chem. Biol. 4(7), 2009
PMID: 19492849
Super-Resolution Imaging with Small Organic Fluorophores
Heilemann, Angewandte Chemie International Edition 48(37), 2009
Real-time computation of subdiffraction-resolution fluorescence images.
Wolter S, Schuttpelz M, Tscherepanow M, VAN DE Linde S, Heilemann M, Sauer M., J Microsc 237(1), 2010
PMID: 20055915
Resolving single-molecule assembled patterns with superresolution blink-microscopy.
Cordes T, Strackharn M, Stahl SW, Summerer W, Steinhauer C, Forthmann C, Puchner EM, Vogelsang J, Gaub HE, Tinnefeld P., Nano Lett. 10(2), 2010
PMID: 20017533


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