Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots

Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D, Kaltschmidt B, Kaltschmidt C, Heilemann M (2010)
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1803(10): 1224-1229.

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Abstract
We introduce semiconductor quantum dot-based fluorescence imaging with similar to 2-fold increased optical resolution in three dimensions as a method that allows both studying cellular structures and spatial organization of biomolecules in membranes and subcellular organelles. Target biomolecules are labelled with quantum dots via immunocytochemistry. The resolution enhancement is achieved by three-photon absorption of quantum dots and subsequent fluorescence emission from a higher-order excitonic state. Different from conventional multiphoton microscopy, this approach can be realized on any confocal microscope without the need for pulsed excitation light. We demonstrate quantum dot triexciton imaging (QDTI) of the microtubule network of U373 cells, 3D imaging of TNF receptor 2 on the plasma membrane of HeLa cells, and multicolor 3D imaging of mitochondrial cytochrome c oxidase and actin in COS-7 cells. (C) 2010 Elsevier B.V. All rights reserved.
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Heidbreder M, Endesfelder U, van de Linde S, et al. Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 2010;1803(10):1224-1229.
Heidbreder, M., Endesfelder, U., van de Linde, S., Hennig, S., Widera, D., Kaltschmidt, B., Kaltschmidt, C., et al. (2010). Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1803(10), 1224-1229.
Heidbreder, M., Endesfelder, U., van de Linde, S., Hennig, S., Widera, D., Kaltschmidt, B., Kaltschmidt, C., and Heilemann, M. (2010). Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1803, 1224-1229.
Heidbreder, M., et al., 2010. Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1803(10), p 1224-1229.
M. Heidbreder, et al., “Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots”, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, vol. 1803, 2010, pp. 1224-1229.
Heidbreder, M., Endesfelder, U., van de Linde, S., Hennig, S., Widera, D., Kaltschmidt, B., Kaltschmidt, C., Heilemann, M.: Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 1803, 1224-1229 (2010).
Heidbreder, Meike, Endesfelder, Ulrike, van de Linde, Sebastian, Hennig, Simon, Widera, Darius, Kaltschmidt, Barbara, Kaltschmidt, Christian, and Heilemann, Mike. “Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots”. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1803.10 (2010): 1224-1229.
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Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots.
Sporbert A, Cseresnyes Z, Heidbreder M, Domaing P, Hauser S, Kaltschmidt B, Kaltschmidt C, Heilemann M, Widera D., PLoS ONE 8(5), 2013
PMID: 23700448

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