The Zur regulon of Corynebacterium glutamicum ATCC 13032

Schröder J, Jochmann N, Rodionov DA, Tauch A (2010)
BMC Genomics 11(1).

Journal Article | Published | English
Background Zinc is considered as an essential element for all living organisms, but it can be toxic at large concentrations. Bacteria therefore tightly regulate zinc metabolism. The Cg2502 protein of Corynebacterium glutamicum was a candidate to control zinc metabolism in this species, since it was classified as metalloregulator of the zinc uptake regulator (Zur) subgroup of the ferric uptake regulator (Fur) family of DNA-binding transcription regulators. Results The cg2502 (zur) gene was deleted in the chromosome of C. glutamicum ATCC 13032 by an allelic exchange procedure to generate the zur-deficient mutant C. glutamicum JS2502. Whole-genome DNA microarray hybridizations and real-time RT-PCR assays comparing the gene expression in C. glutamicum JS2502 with that of the wild-type strain detected 18 genes with enhanced expression in the zur mutant. The expression data were combined with results from cross-genome comparisons of shared regulatory sites, revealing the presence of candidate Zur-binding sites in the mapped promoter regions of five transcription units encoding components of potential zinc ABC-type transporters (cg0041-cg0042/cg0043; cg2911-cg2912-cg2913), a putative secreted protein (cg0040), a putative oxidoreductase (cg0795), and a putative P-loop GTPase of the COG0523 protein family (cg0794). Enhanced transcript levels of the respective genes in C. glutamicum JS2502 were verified by real-time RT-PCR, and complementation of the mutant with a wild-type zur gene reversed the effect of differential gene expression. The zinc-dependent expression of the putative cg0042 and cg2911 operons was detected in vivo with a gfp reporter system. Moreover, the zinc-dependent binding of purified Zur protein to double-stranded 40-mer oligonucleotides containing candidate Zur-binding sites was demonstrated in vitro by DNA band shift assays. Conclusion Whole-genome expression profiling and DNA band shift assays demonstrated that Zur directly represses in a zinc-dependent manner the expression of nine genes organized in five transcription units. Accordingly, the Zur (Cg2502) protein is the key transcription regulator for genes involved in zinc homeostasis in C. glutamicum.
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Schröder J, Jochmann N, Rodionov DA, Tauch A. The Zur regulon of Corynebacterium glutamicum ATCC 13032. BMC Genomics. 2010;11(1).
Schröder, J., Jochmann, N., Rodionov, D. A., & Tauch, A. (2010). The Zur regulon of Corynebacterium glutamicum ATCC 13032. BMC Genomics, 11(1).
Schröder, J., Jochmann, N., Rodionov, D. A., and Tauch, A. (2010). The Zur regulon of Corynebacterium glutamicum ATCC 13032. BMC Genomics 11.
Schröder, J., et al., 2010. The Zur regulon of Corynebacterium glutamicum ATCC 13032. BMC Genomics, 11(1).
J. Schröder, et al., “The Zur regulon of Corynebacterium glutamicum ATCC 13032”, BMC Genomics, vol. 11, 2010.
Schröder, J., Jochmann, N., Rodionov, D.A., Tauch, A.: The Zur regulon of Corynebacterium glutamicum ATCC 13032. BMC Genomics. 11, (2010).
Schröder, Jasmin, Jochmann, Nina, Rodionov, Dmitry A., and Tauch, Andreas. “The Zur regulon of Corynebacterium glutamicum ATCC 13032”. BMC Genomics 11.1 (2010).
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Role of flavohaemoprotein Hmp and nitrate reductase NarGHJI of Corynebacterium glutamicum for coping with nitrite and nitrosative stress.
Platzen L, Koch-Koerfges A, Weil B, Brocker M, Bott M., FEMS Microbiol. Lett. 350(2), 2014
PMID: 24237595

75 References

Data provided by Europe PubMed Central.

Structural insights into HypB, a GTP-binding protein that regulates metal binding.
Gasper R, Scrima A, Wittinghofer A., J. Biol. Chem. 281(37), 2006
PMID: 16807243
A subset of the diverse COG0523 family of putative metal chaperones is linked to zinc homeostasis in all kingdoms of life.
Haas CE, Rodionov DA, Kropat J, Malasarn D, Merchant SS, de Crecy-Lagard V., BMC Genomics 10(), 2009
PMID: 19822009
The Zur of Xanthomonas campestris functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters.
Huang DL, Tang DJ, Liao Q, Li HC, Chen Q, He YQ, Feng JX, Jiang BL, Lu GT, Chen B, Tang JL., Nucleic Acids Res. 36(13), 2008
PMID: 18586823
Characterization of Zur-dependent genes and direct Zur targets in Yersinia pestis.
Li Y, Qiu Y, Gao H, Guo Z, Han Y, Song Y, Du Z, Wang X, Zhou D, Yang R., BMC Microbiol. 9(), 2009
PMID: 19552825

Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
Efficient electrotransformation of corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1.
Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J., Curr. Microbiol. 45(5), 2002
PMID: 12232668
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR., Gene 77(1), 1989
PMID: 2744488
The IclR-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum.
Brune I, Jochmann N, Brinkrolf K, Huser AT, Gerstmeir R, Eikmanns BJ, Kalinowski J, Puhler A, Tauch A., J. Bacteriol. 189(7), 2007
PMID: 17259312
An evaluation framework for statistical tests on microarray data.
Dondrup M, Huser AT, Mertens D, Goesmann A., J. Biotechnol. 140(1-2), 2009
PMID: 19297690
Linking Cytoscape and the corynebacterial reference database CoryneRegNet.
Baumbach J, Apeltsin L., BMC Genomics 9(), 2008
PMID: 18426593
Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW., Nucleic Acids Res. 37(Database issue), 2009
PMID: 18940867
[Software for analyzing bacterial genomes]
Mironov AA, Vinokurova NP, Gel'fand MS., Mol. Biol. (Mosk.) 34(2), 2000
PMID: 10779952
Computer analysis of transcription regulatory patterns in completely sequenced bacterial genomes.
Mironov AA, Koonin EV, Roytberg MA, Gelfand MS., Nucleic Acids Res. 27(14), 1999
PMID: 10390542
WebLogo: a sequence logo generator.
Crooks GE, Hon G, Chandonia JM, Brenner SE., Genome Res. 14(6), 2004
PMID: 15173120
Clustal W and Clustal X version 2.0.
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG., Bioinformatics 23(21), 2007
PMID: 17846036
Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
Grant SG, Jessee J, Bloom FR, Hanahan D., Proc. Natl. Acad. Sci. U.S.A. 87(12), 1990
PMID: 2162051


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