Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress

Dittami SM, Scornet D, Petit J-L, Ségurens B, Da Silva C, Corre E, Dondrup M, Glatting K-H, König R, Strerck L, Rouzé P, et al. (2009)
Genome Biology 10(6).

Download
OA
Journal Article | Published | English
Author
; ; ; ; ; ; ; ; ; ; ;
All
Abstract
Background: Brown algae (Phaeophyceae) are phylogenetically distant from red and green algae and an important component of the coastal ecosystem. They have developed unique mechanisms that allow them to inhabit the intertidal zone, an environment with high levels of abiotic stress. Ectocarpus siliculosus is being established as a genetic and genomic model for the brown algal lineage, but little is known about its response to abiotic stress. Results: Here we examine the transcriptomic changes that occur during the short term acclimation of E. siliculosus to three different abiotic stress conditions (hyposaline, hypersaline and oxidative stress). Our results show that almost 70% of the expressed genes are regulated in response to at least one of these stressors. Although there are several common elements with terrestrial plants, such as repression of growth-related genes, switching from primary production to protein and nutrient recycling processes, and induction of genes involved in vesicular trafficking, many of the stress-regulated genes are either not known to respond to stress in other organisms or are have been found exclusively in E. siliculosus. Conclusions: This first large-scale transcriptomic study of a brown alga demonstrates that, unlike terrestrial plants, E. siliculosus undergoes extensive reprogramming of its transcriptome during the acclimation to mild abiotic stress. We identify several new genes and pathways with a putative function in the stress response and thus pave the way for more detailed investigations of the mechanisms underlying the stress tolerance of brown algae.
Publishing Year
ISSN
PUB-ID

Cite this

Dittami SM, Scornet D, Petit J-L, et al. Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress. Genome Biology. 2009;10(6).
Dittami, S. M., Scornet, D., Petit, J. - L., Ségurens, B., Da Silva, C., Corre, E., Dondrup, M., et al. (2009). Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress. Genome Biology, 10(6).
Dittami, S. M., Scornet, D., Petit, J. - L., Ségurens, B., Da Silva, C., Corre, E., Dondrup, M., Glatting, K. - H., König, R., Strerck, L., et al. (2009). Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress. Genome Biology 10.
Dittami, S.M., et al., 2009. Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress. Genome Biology, 10(6).
S.M. Dittami, et al., “Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress”, Genome Biology, vol. 10, 2009.
Dittami, S.M., Scornet, D., Petit, J.-L., Ségurens, B., Da Silva, C., Corre, E., Dondrup, M., Glatting, K.-H., König, R., Strerck, L., Rouzé, P., Van de Peer, Y., Cock, J.M., Boyen, C., Tonon, T.: Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress. Genome Biology. 10, (2009).
Dittami, Simon M., Scornet, Delphine, Petit, Jean-Louis, Ségurens, Béatrice, Da Silva, Corinne, Corre, Erwan, Dondrup, Michael, Glatting, Karl-Heinz, König, Rainer, Strerck, Lieven, Rouzé, Pierre, Van de Peer, Yves, Cock, J. Marc, Boyen, Catherine, and Tonon, Thierry. “Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress”. Genome Biology 10.6 (2009).
Main File(s)
File Name
Access Level
OA Open Access

This data publication is cited in the following publications:
This publication cites the following data publications:

38 Citations in Europe PMC

Data provided by Europe PubMed Central.

Microbiota Influences Morphology and Reproduction of the Brown Alga Ectocarpus sp.
Tapia JE, Gonzalez B, Goulitquer S, Potin P, Correa JA., Front Microbiol 7(), 2016
PMID: 26941722
Infection of the brown alga Ectocarpus siliculosus by the oomycete Eurychasma dicksonii induces oxidative stress and halogen metabolism.
Strittmatter M, Grenville-Briggs LJ, Breithut L, Van West P, Gachon CM, Kupper FC., Plant Cell Environ. 39(2), 2016
PMID: 25764246
Development of PCR-Based Markers to Determine the Sex of Kelps.
Lipinska AP, Ahmed S, Peters AF, Faugeron S, Cock JM, Coelho SM., PLoS ONE 10(10), 2015
PMID: 26496392
The genome-scale metabolic network of Ectocarpus siliculosus (EctoGEM): a resource to study brown algal physiology and beyond.
Prigent S, Collet G, Dittami SM, Delage L, Ethis de Corny F, Dameron O, Eveillard D, Thiele S, Cambefort J, Boyen C, Siegel A, Tonon T., Plant J. 80(2), 2014
PMID: 25065645
A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae).
Deng Y, Wang X, Guo H, Duan D., Mol. Biol. Rep. 41(1), 2014
PMID: 24293128
Intra-platform comparison of 25-mer and 60-mer oligonucleotide Nimblegen DNA microarrays.
Fenart S, Chabi M, Gallina S, Huis R, Neutelings G, Riviere N, Thomasset B, Hawkins S, Lucau-Danila A., BMC Res Notes 6(), 2013
PMID: 23375116

81 References

Data provided by Europe PubMed Central.

GOLEM: an interactive graph-based gene-ontology navigation and analysis tool.
Sealfon RS, Hibbs MA, Huttenhower C, Myers CL, Troyanskaya OG., BMC Bioinformatics 7(), 2006
PMID: 17032457
The control of the false discovery rate in multiple testing under dependency.
Benjamini Y, Yekutieli D., 2001
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ., Nucleic Acids Res. 25(17), 1997
PMID: 9254694
Cytoscape: a software environment for integrated models of biomolecular interaction networks.
Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B, Ideker T., Genome Res. 13(11), 2003
PMID: 14597658
HECTAR: a method to predict subcellular targeting in heterokonts.
Gschloessl B, Guermeur Y, Cock JM., BMC Bioinformatics 9(), 2008
PMID: 18811941
TMAP: a new email and WWW service for membrane-protein structural predictions.
Milpetz F, Argos P, Persson B., Trends Biochem. Sci. 20(5), 1995
PMID: 7610486

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 19531237
PubMed | Europe PMC

Search this title in

Google Scholar