Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli

Friehs K, Bailey JE (1989)
Journal of Biotechnology 9(4): 305-316.

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The plasmid pJMC40 containing the lon gene was transformed into E. coli HB101 leading to clones with either pJMC40 or a large plasmid designated pJMC40ins. pJMC40ins has a 1.3 kb DNA insert outside of the lon operon. Attempts to reduce the size of pJMC40 through circularization of a 6.7 kb EcoRI fragment containing the lon operon and larger parts of pBR322 failed. Circularization of an 8.0 kb EcoRI fragment of pJMC40ins, consisting of the 6.7 kb fragment and the 1.3 kb insert, produced a new plasmid which is useful as a small vector with a functional lon operon. The lon gene was cloned in vitro into the vector pUC18 setting it under the control of the lac promotor. Different attempts to transform this construct into E. coli failed. We suggest that the expression level of lon is very crucial for the viability of E. coli cells. Changes in regulation of lon expression expected to elevate La activity are lethal for E. coli.
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Friehs K, Bailey JE. Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli. Journal of Biotechnology. 1989;9(4):305-316.
Friehs, K., & Bailey, J. E. (1989). Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli. Journal of Biotechnology, 9(4), 305-316.
Friehs, K., and Bailey, J. E. (1989). Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli. Journal of Biotechnology 9, 305-316.
Friehs, K., & Bailey, J.E., 1989. Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli. Journal of Biotechnology, 9(4), p 305-316.
K. Friehs and J.E. Bailey, “Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli”, Journal of Biotechnology, vol. 9, 1989, pp. 305-316.
Friehs, K., Bailey, J.E.: Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli. Journal of Biotechnology. 9, 305-316 (1989).
Friehs, Karl, and Bailey, J. E. “Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli”. Journal of Biotechnology 9.4 (1989): 305-316.
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