The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator
Heidemann R, Riese U, Lütkemeyer D, Büntemeyer H, Lehmann J (1994)
Cytotechnology 14(1): 1-9.
Download
Journal Article
| Published
| English
Author
Department
Abstract
The production of small quantities of monoclonal antibodies and recombinant proteins was carried out using a new low cost production system, the Super Spinner. Into a 1 1 standard Duran(R) flask a membrane stirrer equipped with a polypropylene hollow fiber membrane was installed to improve the oxygen supply by bubble-free aeration. The aeration was facilitated by using the CO2 conditioned incubator gas, which was pumped through the membrane stirrer via a small membrane pump. The maximal oxygen transfer rate (OTR(max)) of the Super Spinner was detected. For this purpose one spinner flask was equipped with an oxygen electrode. The OTR(max) was measured by the dynamic method. The ratio of membrane length to culture volume was adapted corresponding to the oxygen uptake rate of the cells according to the desired cell density. A balanced nutrient supply resulted in an optimal formation and yield of products.
Keywords
Publishing Year
ISSN
eISSN
PUB-ID
Cite this
Heidemann R, Riese U, Lütkemeyer D, Büntemeyer H, Lehmann J. The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology. 1994;14(1):1-9.
Heidemann, R., Riese, U., Lütkemeyer, D., Büntemeyer, H., & Lehmann, J. (1994). The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology, 14(1), 1-9. doi:10.1007/BF00772190
Heidemann, R., Riese, U., Lütkemeyer, D., Büntemeyer, H., and Lehmann, J. (1994). The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology 14, 1-9.
Heidemann, R., et al., 1994. The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology, 14(1), p 1-9.
R. Heidemann, et al., “The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator”, Cytotechnology, vol. 14, 1994, pp. 1-9.
Heidemann, R., Riese, U., Lütkemeyer, D., Büntemeyer, H., Lehmann, J.: The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology. 14, 1-9 (1994).
Heidemann, R., Riese, U., Lütkemeyer, Dirk, Büntemeyer, Heino, and Lehmann, Jürgen. “The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator”. Cytotechnology 14.1 (1994): 1-9.
All files available under the following license(s):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Main File(s)
File Name
Access Level
Open Access
This data publication is cited in the following publications:
This publication cites the following data publications:
19 Citations in Europe PMC
Data provided by Europe PubMed Central.
A membrane stirrer for product recovery and substrate feeding.
Femmer T, Carstensen F, Wessling M., Biotechnol Bioeng 112(2), 2015
PMID: 25212847
Femmer T, Carstensen F, Wessling M., Biotechnol Bioeng 112(2), 2015
PMID: 25212847
A case study in converting disposable process scouting devices into disposable bioreactors as a future bioprocessing tool.
Vallejos JR, Uplekar S, da Silva JF, Brorson KA, Moreira AR, Rao G., Biotechnol Bioeng 109(11), 2012
PMID: 22549482
Vallejos JR, Uplekar S, da Silva JF, Brorson KA, Moreira AR, Rao G., Biotechnol Bioeng 109(11), 2012
PMID: 22549482
Mini-scale bioprocessing systems for highly parallel animal cell cultures.
Kim BJ, Diao J, Shuler ML., Biotechnol Prog 28(3), 2012
PMID: 22522970
Kim BJ, Diao J, Shuler ML., Biotechnol Prog 28(3), 2012
PMID: 22522970
Technological progresses in monoclonal antibody production systems.
Rodrigues ME, Costa AR, Henriques M, Azeredo J, Oliveira R., Biotechnol Prog 26(2), 2010
PMID: 20043321
Rodrigues ME, Costa AR, Henriques M, Azeredo J, Oliveira R., Biotechnol Prog 26(2), 2010
PMID: 20043321
Glycosphingolipids in vascular endothelial cells: relationship of heterogeneity in Gb3Cer/CD77 receptor expression with differential Shiga toxin 1 cytotoxicity.
Schweppe CH, Bielaszewska M, Pohlentz G, Friedrich AW, Büntemeyer H, Schmidt MA, Kim KS, Peter-Katalinić J, Karch H, Müthing J., Glycoconj J 25(4), 2008
PMID: 18176841
Schweppe CH, Bielaszewska M, Pohlentz G, Friedrich AW, Büntemeyer H, Schmidt MA, Kim KS, Peter-Katalinić J, Karch H, Müthing J., Glycoconj J 25(4), 2008
PMID: 18176841
Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000 L by surface aeration with air.
Zhang X, Stettler M, Reif O, Kocourek A, Dejesus M, Hacker DL, Wurm FM., N Biotechnol 25(1), 2008
PMID: 18504021
Zhang X, Stettler M, Reif O, Kocourek A, Dejesus M, Hacker DL, Wurm FM., N Biotechnol 25(1), 2008
PMID: 18504021
Serum-free production of a chimeric E-selectin-IgG protein from 1 to 100 l scale: Repeated batch cultivation versus continuous spin filter perfusion.
Werner A, Lütkemeyer D, Poggendorf I, Lehmann J, Müthing J., Cytotechnology 38(1-3), 2002
PMID: 19003086
Werner A, Lütkemeyer D, Poggendorf I, Lehmann J, Müthing J., Cytotechnology 38(1-3), 2002
PMID: 19003086
Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding.
Böhme C, Nimtz M, Grabenhorst E, Conradt HS, Strathmann A, Ragg H., Eur J Biochem 269(3), 2002
PMID: 11846800
Böhme C, Nimtz M, Grabenhorst E, Conradt HS, Strathmann A, Ragg H., Eur J Biochem 269(3), 2002
PMID: 11846800
Preferential binding of the anticancer drug rViscumin (recombinant mistletoe lectin) to terminally alpha2-6-sialylated neolacto-series gangliosides.
Müthing J, Burg M, Möckel B, Langer M, Metelmann-Strupat W, Werner A, Neumann U, Peter-Katalinic J, Eck J., Glycobiology 12(8), 2002
PMID: 12145189
Müthing J, Burg M, Möckel B, Langer M, Metelmann-Strupat W, Werner A, Neumann U, Peter-Katalinic J, Eck J., Glycobiology 12(8), 2002
PMID: 12145189
An automatic system for the assessment of complex medium additives under cultivation conditions.
Iding K, Büntemeyer H, Gudermann F, Deutschmann SM, Kionka C, Lehmann J., Biotechnol Bioeng 73(6), 2001
PMID: 11344448
Iding K, Büntemeyer H, Gudermann F, Deutschmann SM, Kionka C, Lehmann J., Biotechnol Bioeng 73(6), 2001
PMID: 11344448
Perfusion culture of baculovirus-infected BTI-Tn-5B1-4 insect cells: a method to restore cell-specific beta-trace glycoprotein productivity at high cell density.
Chico E, Jäger V., Biotechnol Bioeng 70(5), 2000
PMID: 11042554
Chico E, Jäger V., Biotechnol Bioeng 70(5), 2000
PMID: 11042554
Estimation of cell damage in bench- and pilot-scale affinity expanded-bed chromatography for the purification of monoclonal antibodies.
Lütkemeyer D, Ameskamp N, Tebbe H, Wittler J, Lehmann J., Biotechnol Bioeng 65(1), 1999
PMID: 10440678
Lütkemeyer D, Ameskamp N, Tebbe H, Wittler J, Lehmann J., Biotechnol Bioeng 65(1), 1999
PMID: 10440678
Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures.
Heidemann R, Lütkemeyer D, Büntemeyer H, Lehmann J., Cytotechnology 26(3), 1998
PMID: 22358616
Heidemann R, Lütkemeyer D, Büntemeyer H, Lehmann J., Cytotechnology 26(3), 1998
PMID: 22358616
Cell size distribution as a parameter for the predetermination of exponential growth during repeated batch cultivation of CHO cells.
Seewöster T, Lehmann J., Biotechnol Bioeng 55(5), 1997
PMID: 18636589
Seewöster T, Lehmann J., Biotechnol Bioeng 55(5), 1997
PMID: 18636589
Production of recombinant human antithrombin III on 20-L bioreactor scale: correlation of supernatant neuraminidase activity, desialylation, and decrease of biological activity of recombinant glycoprotein.
Munzert E, Heidemann R, Büntemeyer H, Lehmann J, Müthing J., Biotechnol Bioeng 56(4), 1997
PMID: 18642246
Munzert E, Heidemann R, Büntemeyer H, Lehmann J, Müthing J., Biotechnol Bioeng 56(4), 1997
PMID: 18642246
Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified recombinant enzyme.
Knecht W, Bergjohann U, Gonski S, Kirschbaum B, Löffler M., Eur J Biochem 240(1), 1996
PMID: 8925840
Knecht W, Bergjohann U, Gonski S, Kirschbaum B, Löffler M., Eur J Biochem 240(1), 1996
PMID: 8925840
Production of tissue plasminogen activator (tPA) in two and three dimensionally growing cultures of Bowes melanoma cells.
Brenner J, Hülser DF., Biotechnol Bioeng 51(4), 1996
PMID: 18629794
Brenner J, Hülser DF., Biotechnol Bioeng 51(4), 1996
PMID: 18629794
Propagation of Spodoptera frugiperda cells (Sf9) and production of recombinant proteins with the baculovirus expression system using improved spinner flasks with membrane aeration.
Jager V, Kobold A., Biotechnology techniques. 9(6), 1995
PMID: IND20470348
Jager V, Kobold A., Biotechnology techniques. 9(6), 1995
PMID: IND20470348
Design of a bubble-swarm bioreactor for animal cell culture.
Gudermann F, Lütkemeyer D, Lehmann J., Cytotechnology 15(1-3), 1994
PMID: 7765944
Gudermann F, Lütkemeyer D, Lehmann J., Cytotechnology 15(1-3), 1994
PMID: 7765944
18 References
Data provided by Europe PubMed Central.
AUTHOR UNKNOWN, 0
Optimization of serum-free fermentation processes for antibody production.
Buntemeyer H, Lutkemeyer D, Lehmann J., Cytotechnology 5(1), 1991
PMID: 1367052
Buntemeyer H, Lutkemeyer D, Lehmann J., Cytotechnology 5(1), 1991
PMID: 1367052
A three-phase pattern in growth, monoclonal antibody production, and metabolite exchange in a hybridoma bioreactor culture.
Bushell ME, Bell SL, Scott MF, Snell K, Spier RE, Wardell JN, Sanders PG., Biotechnol. Bioeng. 42(1), 1993
PMID: 18609657
Bushell ME, Bell SL, Scott MF, Snell K, Spier RE, Wardell JN, Sanders PG., Biotechnol. Bioeng. 42(1), 1993
PMID: 18609657
M, Biotechnol. Bioeng. 33(), 1990
F, Proc. Miami Winter Symp. 8(), 1988
R, Eur. J. Cell Biol. 60(Suppl. 37), 1993
AUTHOR UNKNOWN, 0
V, Cytotechnology 1(), 1988
J, Dev. Biol. Standard. 66(), 1987
J, Animal Cell Biotechnology 3(), 1988
J, BioEngineering 5+6(), 1992
OW, Develop. Biol. Standard 60(), 1985
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
R, J. Immunol. Methodes 146(), 1992
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
G, Biotechnology 5(), 1987
Export
0 Marked PublicationsWeb of Science
View record in Web of Science®Sources
PMID: 7765107
PubMed | Europe PMC
Google Scholar