The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator

Heidemann R, Riese U, Lütkemeyer D, Büntemeyer H, Lehmann J (1994)
Cytotechnology 14(1): 1-9.

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urn:nbn:de:0070-pub-17824041
Journal Article | Published | English
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Technische Fakultät
Abstract
The production of small quantities of monoclonal antibodies and recombinant proteins was carried out using a new low cost production system, the Super Spinner. Into a 1 1 standard Duran(R) flask a membrane stirrer equipped with a polypropylene hollow fiber membrane was installed to improve the oxygen supply by bubble-free aeration. The aeration was facilitated by using the CO2 conditioned incubator gas, which was pumped through the membrane stirrer via a small membrane pump. The maximal oxygen transfer rate (OTR(max)) of the Super Spinner was detected. For this purpose one spinner flask was equipped with an oxygen electrode. The OTR(max) was measured by the dynamic method. The ratio of membrane length to culture volume was adapted corresponding to the oxygen uptake rate of the cells according to the desired cell density. A balanced nutrient supply resulted in an optimal formation and yield of products.
Keywords
ANTIBODIES ; SUPER-SPINNER ; RECOMBINANT CHO CELL LINES ; MEMBRANE AERATION ; T-PA ; AT III ; CO2 INCUBATOR ; HYBRIDOMAS ; MONOCLONAL
Publishing Year
1994
ISSN
0920-9069
eISSN
1573-0778
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Heidemann R, Riese U, Lütkemeyer D, Büntemeyer H, Lehmann J. The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology. 1994;14(1):1-9.
Heidemann, R., Riese, U., Lütkemeyer, D., Büntemeyer, H., & Lehmann, J. (1994). The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology, 14(1), 1-9. doi:10.1007/BF00772190
Heidemann, R., Riese, U., Lütkemeyer, D., Büntemeyer, H., and Lehmann, J. (1994). The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology 14, 1-9.
Heidemann, R., et al., 1994. The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology, 14(1), p 1-9.
R. Heidemann, et al., “The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator”, Cytotechnology, vol. 14, 1994, pp. 1-9.
Heidemann, R., Riese, U., Lütkemeyer, D., Büntemeyer, H., Lehmann, J.: The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology. 14, 1-9 (1994).
Heidemann, R., Riese, U., Lütkemeyer, Dirk, Büntemeyer, Heino, and Lehmann, Jürgen. “The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator”. Cytotechnology 14.1 (1994): 1-9.
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