The superimmunity gene sim of bacteriophage P1 causes superinfection exclusion

Kliem M, Dreiseikelmann B (1989)
Virology 171(2): 350-355.

Download
OA
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Volltext vorhanden für diesen Nachweis
Autor
Abstract / Bemerkung
Previous work has shown that the sim gene of bacteriophage P1, if cloned into a multicopy vector, confers immunity against P1 infection to cells. We show that a 1.85-kb DNA fragment from the sim region of P1 (in the multicopy plasmid pMK4) expresses immunity and encodes three proteins with molecular weights of about 25, 24, and 15 kDa. Deletion of 650 bp from the sim region abolished synthesis of all three proteins and of the sim phenotype. Expression of sim did not prevent adsorption of P1 to cells. Successful transfection with linear P1 DNA suggests that the recombinational circularization of P1 DNA is not inhibited in the presence of sim. Plasmid pMK4 and a P1 prophage can be induced maintained in the cell indicating that replication of the prophage is not disturbed by sim. The prophage can be induced in the presence of sim. This shows that the sim phenotype is not caused by preventing lytic replication or phage maturation. In cells with pMK4 there is no expression of genes from infecting phages and transduction frequency is drastically reduced. We suggest that sim functions as a superinfection exclusion system by preventing transfer of DNA from the adsorbed phages into the cytoplasm.
Erscheinungsjahr
Zeitschriftentitel
Virology
Band
171
Zeitschriftennummer
2
Seite
350-355
ISSN
PUB-ID

Zitieren

Kliem M, Dreiseikelmann B. The superimmunity gene sim of bacteriophage P1 causes superinfection exclusion. Virology. 1989;171(2):350-355.
Kliem, M., & Dreiseikelmann, B. (1989). The superimmunity gene sim of bacteriophage P1 causes superinfection exclusion. Virology, 171(2), 350-355. doi:10.1016/0042-6822(89)90602-8
Kliem, M., and Dreiseikelmann, B. (1989). The superimmunity gene sim of bacteriophage P1 causes superinfection exclusion. Virology 171, 350-355.
Kliem, M., & Dreiseikelmann, B., 1989. The superimmunity gene sim of bacteriophage P1 causes superinfection exclusion. Virology, 171(2), p 350-355.
M. Kliem and B. Dreiseikelmann, “The superimmunity gene sim of bacteriophage P1 causes superinfection exclusion”, Virology, vol. 171, 1989, pp. 350-355.
Kliem, M., Dreiseikelmann, B.: The superimmunity gene sim of bacteriophage P1 causes superinfection exclusion. Virology. 171, 350-355 (1989).
Kliem, Manfred, and Dreiseikelmann, Brigitte. “The superimmunity gene sim of bacteriophage P1 causes superinfection exclusion”. Virology 171.2 (1989): 350-355.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2016-09-05T14:47:31Z

16 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

RNA Silencing May Play a Role in but Is Not the Only Determinant of the Multiplicity of Infection.
Donaire L, Burgyán J, García-Arenal F., J Virol 90(1), 2016
PMID: 26491166
Cellular responses in Bacillus thuringiensis CS33 during bacteriophage BtCS33 infection.
Wu D, Yuan Y, Liu P, Wu Y, Gao M., J Proteomics 101(), 2014
PMID: 24565692
Bacteriophage 434 Hex protein prevents recA-mediated repressor autocleavage.
Shkilnyj P, Colon MP, Koudelka GB., Viruses 5(1), 2013
PMID: 23303392
A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein.
Julve JM, Gandía A, Fernández-Del-Carmen A, Sarrion-Perdigones A, Castelijns B, Granell A, Orzaez D., Plant Mol Biol 81(6), 2013
PMID: 23417583
A systematic approach to virus-virus interactions.
DaPalma T, Doonan BP, Trager NM, Kasman LM., Virus Res 149(1), 2010
PMID: 20093154
Bacteriophage resistance mechanisms.
Labrie SJ, Samson JE, Moineau S., Nat Rev Microbiol 8(5), 2010
PMID: 20348932
Inhibition of superinfection and the evolution of viral latency.
Berngruber TW, Weissing FJ, Gandon S., J Virol 84(19), 2010
PMID: 20660193
Global changes in cellular gene expression during bacteriophage PRD1 infection.
Poranen MM, Ravantti JJ, Grahn AM, Gupta R, Auvinen P, Bamford DH., J Virol 80(16), 2006
PMID: 16873264
Genome of bacteriophage P1.
Łobocka MB, Rose DJ, Plunkett G, Rusin M, Samojedny A, Lehnherr H, Yarmolinsky MB, Blattner FR., J Bacteriol 186(21), 2004
PMID: 15489417

26 References

Daten bereitgestellt von Europe PubMed Central.

The c4 gene of phage P1.
Baumstark BR, Scott JR., Virology 156(2), 1987
PMID: 3811234
Role of antirepressor in the bipartite control of repression and immunity by bacteriophage P22.
Botstein K, Lew KK, Jarvik V, Swanson CA., J. Mol. Biol. 91(4), 1975
PMID: 1097697
Superimmunity: characterization of a new gene in the immunity region of P1.
Devlin BH, Baumstark BR, Scott JR., Virology 120(2), 1982
PMID: 6285609
The c1 repressor of bacteriophage P1. Isolation and characterization of the repressor protein.
Dreiseikelmann B, Velleman M, Schuster H., J. Biol. Chem. 263(3), 1988
PMID: 2826478
Dual control of lysogeny by bacteriophage P22: an antirepressor locus and its controlling elements.
Levine M, Truesdell S, Ramakrishnan T, Bronson MJ., J. Mol. Biol. 91(4), 1975
PMID: 1097696
Bacteriophage Mu encoded polypeptides synthesized in infected minicells
Magazin, FEMS Microbiol. Lett. 4(), 1978
Calcium-dependent bacteriophage DNA infection.
Mandel M, Higa A., J. Mol. Biol. 53(1), 1970
PMID: 4922220

Maniatis, 1982
Use of Minicells for Bacteriophage Directed Polypeptide Biosynthesis
Reeve, 1979
Immunity and repression in bacteriophages P1 and P7.
Scott JR., Curr. Top. Microbiol. Immunol. 90(), 1980
PMID: 7000446
Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites.
Sternberg N, Hamilton D., J. Mol. Biol. 150(4), 1981
PMID: 6276557
Site-specific recombination and its role in the life cycle of bacteriophage P1
Sternberg, 1981
The molecular genetics of bacteriophage P1.
Sternberg N, Hoess R., Annu. Rev. Genet. 17(), 1983
PMID: 6364958
Mechanism of action of Salmonella phage P22 antirepressor.
Susskind MM, Botstein D., J. Mol. Biol. 98(2), 1975
PMID: 1104871
Bacteriophage P1
Yarmolinsky, 1987

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 2763457
PubMed | Europe PMC

Suchen in

Google Scholar