A dnaB-like protein of Pseudomonas aeruginosa

Dreiseikelmann B, Riedel H-D, Schuster H (1987)
Nucleic Acids Research 15(2): 385-395.

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Journal Article | Published | English
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Abstract
A dnaB-like protein from P. aeruginosa was purified to near homogeneity using as an assay the immunoprecipitation by E. coli dnaB antiserum in a solid-phase. In the chromatographic characteristics including the affinity to immobilized ATP the dnaB-like protein of P. aeruginosa is similar to the dnaB protein of E. coli with the exception that it does not bind to heparin-Sepharose. The dnaB-like protein has a native molecular weight of about 320,000 as determined by glycerol gradient sedimentation. It consists of several identical subunits of molecular weight of 56,000 as measured in a denaturing SDS gel. Associated with the enzyme is a DNA-dependent ATPase- and helicase activity. The dnaB-like protein is similar to the E. coli dnaB protein with regard to the binding of ATP gamma S and the formation of a ternary complex consisting of the enzyme, ATP gamma S, and phi X174 DNA. However, the enzyme of P. aeruginosa is inactive in a phi X174 DNA-dependent in vitro dnaB complementation assay using an E. coli dnaBts extract.
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Dreiseikelmann B, Riedel H-D, Schuster H. A dnaB-like protein of Pseudomonas aeruginosa. Nucleic Acids Research. 1987;15(2):385-395.
Dreiseikelmann, B., Riedel, H. - D., & Schuster, H. (1987). A dnaB-like protein of Pseudomonas aeruginosa. Nucleic Acids Research, 15(2), 385-395.
Dreiseikelmann, B., Riedel, H. - D., and Schuster, H. (1987). A dnaB-like protein of Pseudomonas aeruginosa. Nucleic Acids Research 15, 385-395.
Dreiseikelmann, B., Riedel, H.-D., & Schuster, H., 1987. A dnaB-like protein of Pseudomonas aeruginosa. Nucleic Acids Research, 15(2), p 385-395.
B. Dreiseikelmann, H.-D. Riedel, and H. Schuster, “A dnaB-like protein of Pseudomonas aeruginosa”, Nucleic Acids Research, vol. 15, 1987, pp. 385-395.
Dreiseikelmann, B., Riedel, H.-D., Schuster, H.: A dnaB-like protein of Pseudomonas aeruginosa. Nucleic Acids Research. 15, 385-395 (1987).
Dreiseikelmann, Brigitte, Riedel, Hans-Dieter, and Schuster, Heinz. “A dnaB-like protein of Pseudomonas aeruginosa”. Nucleic Acids Research 15.2 (1987): 385-395.
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