Tissue specificity of the initiation of immunoglobulin k gene transcription

Falkner FG, Neumann E, Zachau HG (1984)
Hoppe-Seyler's Zeitschrift für physiologische Chemie 365: 1331-1343.

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Journal Article | Published | English
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Abstract
The transient transcription of a rearranged mouse immunoglobulin k gene was studied in a monkey fibroblast cell line. The gene was inserted into an SV40 expression vector and the calcium phosphate coprecipitation method was used for transfection. The transcripts were correctly spliced; transcription, however, was initiated within the vector and not at the correct site 23-26 bp upstream of the gene, irrespective of the length of the upstream sequences (90, 160, 370, and 870 bp) in the plasmid constructs. In contrast, accurately initiated transcripts were observed when a plasmid containing the k gene with 870 bp of its upstream sequence was introduced into a lymphoid cell line; the plasmid was constructed from the pSV2-gpt vector and the electric impulse method was used fro gene transfer in most experiments. Tissue-specific expression of k light chain genes in lymphoid cells is known to depend on the presence of an enhancer element in the J-C intron. The results reported in this paper suggest that the sequence elements pd and dc which are located upstream of the leader gene segment also act in a tissue-specific manner ant that it is the initiation of transcription which is a tissue-specific event.
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Falkner FG, Neumann E, Zachau HG. Tissue specificity of the initiation of immunoglobulin k gene transcription. Hoppe-Seyler's Zeitschrift für physiologische Chemie. 1984;365:1331-1343.
Falkner, F. G., Neumann, E., & Zachau, H. G. (1984). Tissue specificity of the initiation of immunoglobulin k gene transcription. Hoppe-Seyler's Zeitschrift für physiologische Chemie, 365, 1331-1343.
Falkner, F. G., Neumann, E., and Zachau, H. G. (1984). Tissue specificity of the initiation of immunoglobulin k gene transcription. Hoppe-Seyler's Zeitschrift für physiologische Chemie 365, 1331-1343.
Falkner, F.G., Neumann, E., & Zachau, H.G., 1984. Tissue specificity of the initiation of immunoglobulin k gene transcription. Hoppe-Seyler's Zeitschrift für physiologische Chemie, 365, p 1331-1343.
F.G. Falkner, E. Neumann, and H.G. Zachau, “Tissue specificity of the initiation of immunoglobulin k gene transcription”, Hoppe-Seyler's Zeitschrift für physiologische Chemie, vol. 365, 1984, pp. 1331-1343.
Falkner, F.G., Neumann, E., Zachau, H.G.: Tissue specificity of the initiation of immunoglobulin k gene transcription. Hoppe-Seyler's Zeitschrift für physiologische Chemie. 365, 1331-1343 (1984).
Falkner, Falko G., Neumann, Eberhard, and Zachau, Hans G. “Tissue specificity of the initiation of immunoglobulin k gene transcription”. Hoppe-Seyler's Zeitschrift für physiologische Chemie 365 (1984): 1331-1343.
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