Gene transfer into mouse lyoma cells by electroporation in high electric fields

Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH (1982)
EMBO Journal 1(7): 841-845.

Download
OA
Journal Article | Published | English
Author
; ; ;
Abstract
Electric impulses (8 kV/cm, 5 microseconds) were found to increase greatly the uptake of DNA into cells. When linear or circular plasmid DNA containing the herpes simplex thymidine kinase (TK) gene is added to a suspension of mouse L cells deficient in the TK gene and the cells are then exposed to electric fields, stable transformants are formed that survive in the HAT selection medium. At 20 degrees C after the application of three successive electric impulses followed by 10 min to allow DNA entry there result 95 (+/- 3) transformants per 10(6) cells and per 1.2 micrograms DNA. Compared with biochemical techniques, the electric field method of gene transfer is very simple, easily applicable, and very efficient. Because the mechanism of DNA transport through cell membranes is not known, a simple physical model for the enhanced DNA penetration into cells in high electric fields is proposed. According to this ' electroporation model' the interaction of the external electric field with the lipid dipoles of a pore configuration induces and stabilizes the permeation sites and thus enhances cross membrane transport.
Publishing Year
PUB-ID

Cite this

Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO Journal. 1982;1(7):841-845.
Neumann, E., Schaefer-Ridder, M., Wang, Y., & Hofschneider, P. H. (1982). Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO Journal, 1(7), 841-845.
Neumann, E., Schaefer-Ridder, M., Wang, Y., and Hofschneider, P. H. (1982). Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO Journal 1, 841-845.
Neumann, E., et al., 1982. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO Journal, 1(7), p 841-845.
E. Neumann, et al., “Gene transfer into mouse lyoma cells by electroporation in high electric fields”, EMBO Journal, vol. 1, 1982, pp. 841-845.
Neumann, E., Schaefer-Ridder, M., Wang, Y., Hofschneider, P.H.: Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO Journal. 1, 841-845 (1982).
Neumann, Eberhard, Schaefer-Ridder, M., Wang, Y., and Hofschneider, P. H. “Gene transfer into mouse lyoma cells by electroporation in high electric fields”. EMBO Journal 1.7 (1982): 841-845.
Main File(s)
Access Level
OA Open Access

This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Search this title in

Google Scholar