Electric field mediated gene transfer

Wong T-K, Neumann E (1982)
Biochemical and Biophysical Research Communication 107(2): 584-587.

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Abstract / Bemerkung
A simple physical chemical method of transferring genes into eucaryotic cells is described. Electric impulses in the intensity range of 5–10 kV/cm with a duration of 5–10 [mu]s were found to appreciably increase the uptake of DNA into cells. After electric field treatment, stable transformants were obtained in a system containing mouse cells deficient in thymidine kinase and a plasmid DNA harbouring the Herpes simplex thymidine kinase gene. The efficiency of transformation well compares with the results of biochemical methods of gene transfer. The electric field technique appears unique in its ease and simplicity.
Erscheinungsjahr
Zeitschriftentitel
Biochemical and Biophysical Research Communication
Band
107
Zeitschriftennummer
2
Seite
584-587
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PUB-ID

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Wong T-K, Neumann E. Electric field mediated gene transfer. Biochemical and Biophysical Research Communication. 1982;107(2):584-587.
Wong, T. - K., & Neumann, E. (1982). Electric field mediated gene transfer. Biochemical and Biophysical Research Communication, 107(2), 584-587. doi:10.1016/0006-291X(82)91531-5
Wong, T. - K., and Neumann, E. (1982). Electric field mediated gene transfer. Biochemical and Biophysical Research Communication 107, 584-587.
Wong, T.-K., & Neumann, E., 1982. Electric field mediated gene transfer. Biochemical and Biophysical Research Communication, 107(2), p 584-587.
T.-K. Wong and E. Neumann, “Electric field mediated gene transfer”, Biochemical and Biophysical Research Communication, vol. 107, 1982, pp. 584-587.
Wong, T.-K., Neumann, E.: Electric field mediated gene transfer. Biochemical and Biophysical Research Communication. 107, 584-587 (1982).
Wong, Tai-Kin, and Neumann, Eberhard. “Electric field mediated gene transfer”. Biochemical and Biophysical Research Communication 107.2 (1982): 584-587.
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111 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Electroporation for the efficient transfection of mammalian cells with DNA.
Chu G, Hayakawa H, Berg P., Nucleic Acids Res 15(3), 1987
PMID: 3029703
Electronic genetic¿physical and biological aspects of cellular electromanipulation.
Hofmann GA, Evans GA., IEEE Eng Med Biol Mag 5(4), 1986
PMID: 19493809
Inhibition of gene expression in plant cells by expression of antisense RNA.
Ecker JR, Davis RW., Proc Natl Acad Sci U S A 83(15), 1986
PMID: 16593734
Characterization, expression, and evolution of the mouse embryonic zeta-globin gene.
Leder A, Weir L, Leder P., Mol Cell Biol 5(5), 1985
PMID: 4000117
Gene transfer into mouse lyoma cells by electroporation in high electric fields.
Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH., EMBO J 1(7), 1982
PMID: 6329708

14 References

Daten bereitgestellt von Europe PubMed Central.

A new technique for the assay of infectivity of human adenovirus 5 DNA.
Graham FL, van der Eb AJ., Virology 52(2), 1973
PMID: 4705382

Diacumakos, 1973
Splicing and the formation of stable RNA.
Hamer DH, Leder P., Cell 18(4), 1979
PMID: 229971
Introduction of liposome-encapsulated SV40 DNA into cells.
Fraley R, Subramani S, Berg P, Papahadjopoulos D., J. Biol. Chem. 255(21), 1980
PMID: 6253474
Liposomes as gene carriers: efficient transformation of mouse L cells by thymidine kinase gene.
Schaefer-Ridder M, Wang Y, Hofschneider PH., Science 215(4529), 1982
PMID: 7053567
DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.
KIT S, DUBBS DR, PIEKARSKI LJ, HSU TC., Exp. Cell Res. 31(), 1963
PMID: 14056989

Weissmann, 1979

Neumann, Naturwissenschaften 67(), 1980
Permeability changes induced by electric impulses in vesicular membranes.
Neumann E, Rosenheck K., J. Membr. Biol. 10(3), 1972
PMID: 4667921

Szybalski, 1962
Transcellular ion flow in Escherichia coli B and electrical sizing of bacterias.
Zimmermann U, Schulz J, Pilwat G., Biophys. J. 13(10), 1973
PMID: 4583964
Effects of high electric fields on micro-organisms. 3. Lysis of erythrocytes and protoplasts.
Sale AJ, Hamilton WA., Biochim. Biophys. Acta 163(1), 1968
PMID: 4969954

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