Permeability changes induced by electric impulses in vesicular membranes

Neumann E, Rosenheck K (1972)
Journal of Membrane Biology 10(1): 279-290.

Journal Article | Published | English
Electric impulses were found to cause transient permeability changes in the membranes of vesicles storing biogenic amines. Release of catecholamines induced by electric fields (of the order of 20 kV/cm and decaying exponentially with a decay time of about 150 [mu]sec) was studied, using the chromaffin granules of bovine adrenomedullary cells as a vesicular model system. Far-UV-absorption spectroscopy was applied to determine the amount of catecholamines released from suspended vesicles. A polarization mechanism is suggested for the induction of short-lived permeability changes caused by electric fields. Such transient changes in permeability may possibly represent a part of the sequence of events leading to stimulated neurohumoral secretion.
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Neumann E, Rosenheck K. Permeability changes induced by electric impulses in vesicular membranes. Journal of Membrane Biology. 1972;10(1):279-290.
Neumann, E., & Rosenheck, K. (1972). Permeability changes induced by electric impulses in vesicular membranes. Journal of Membrane Biology, 10(1), 279-290. doi:10.1007/BF01867861
Neumann, E., and Rosenheck, K. (1972). Permeability changes induced by electric impulses in vesicular membranes. Journal of Membrane Biology 10, 279-290.
Neumann, E., & Rosenheck, K., 1972. Permeability changes induced by electric impulses in vesicular membranes. Journal of Membrane Biology, 10(1), p 279-290.
E. Neumann and K. Rosenheck, “Permeability changes induced by electric impulses in vesicular membranes”, Journal of Membrane Biology, vol. 10, 1972, pp. 279-290.
Neumann, E., Rosenheck, K.: Permeability changes induced by electric impulses in vesicular membranes. Journal of Membrane Biology. 10, 279-290 (1972).
Neumann, Eberhard, and Rosenheck, Kurt. “Permeability changes induced by electric impulses in vesicular membranes”. Journal of Membrane Biology 10.1 (1972): 279-290.
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15 References

Data provided by Europe PubMed Central.

Bloom, Neurosci. Res. Prog. Bull. 8(), 1970

Eigen, 1963

Euler, Science 173(), 1970
An improved method for the large-scale isolation of chromaffin granules from bovine adrenal medulla.
Helle KB, Flatmark T, Serck-Hanssen G, Lonning S., Biochim. Biophys. Acta 226(1), 1971
PMID: 4323695

Kornberg, Proc. Nat. Acad. Sci. 68(), 1971
Protein measurement with the Folin phenol reagent.
LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ., J. Biol. Chem. 193(1), 1951
PMID: 14907713

Neumann, Proc. Nat. Acad. Sci. 69(), 1972

Schwarz, J. Phys. Chem. 66(), 1962

Smith, 1968

Stein, 1967

Trifaró, Analyt. Biochem. 34(), 1970

Wiersema, J. Colloid Interface Sci. 22(), 1966


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