Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors

Hillemann D, Pühler A, Wohlleben W (1991)
Nucleic Acids Research 19(4): 727-731.

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For the isolation of single stranded plasmid DNA, various E. coli and E. coli- Streptomyces shuttle plasmids were equipped with the f1 replication origin. The transformation of some representative Streptomyces species with plasmid vectors occurred irrespective of whether single or double stranded DNA was used. In contrast, the transformation of Streptomyces was 10 to 100 times more efficient when an integration vector was in the single stranded form as opposed to the double stranded form. Streptomyces viridochromogenes was transformed by single stranded DNA integration vectors in order to replace the pat by the tsr gene and generate mutants unable to synthesize phosphinothricin - tripeptide (PTT).
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Hillemann D, Pühler A, Wohlleben W. Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research. 1991;19(4):727-731.
Hillemann, D., Pühler, A., & Wohlleben, W. (1991). Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research, 19(4), 727-731.
Hillemann, D., Pühler, A., and Wohlleben, W. (1991). Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research 19, 727-731.
Hillemann, D., Pühler, A., & Wohlleben, W., 1991. Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research, 19(4), p 727-731.
D. Hillemann, A. Pühler, and W. Wohlleben, “Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors”, Nucleic Acids Research, vol. 19, 1991, pp. 727-731.
Hillemann, D., Pühler, A., Wohlleben, W.: Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Research. 19, 727-731 (1991).
Hillemann, Doris, Pühler, Alfred, and Wohlleben, Wolfgang. “Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors”. Nucleic Acids Research 19.4 (1991): 727-731.
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