In vivo two-photon laser-scanning microscopy of Ca 2+ dynamics in visual motion-sensitive neurons

Kalb J, Nielsen T, Fricke M, Egelhaaf M, Kurtz R (2004)
Biochemical and biophysical research communications 316(2): 341-347.

Download
OA
Journal Article | Published | English
Author
; ; ; ;
Abstract
We applied two-photon laser-scanning microscopy (TPLSM) to motion-sensitive visual interneurons of the fly to study Ca(2+) dynamics in vivo at a higher spatial and temporal resolution than possible with conventional fluorescence microscopy. Based on a custom-built two-photon microscope, we performed line scans to measure changes in presynaptic Ca(2+) concentrations elicited by visual stimulation. We used a fast avalanche photodiode (APD) with a high quantum efficiency to detect even low levels of emitted fluorescence. Our experiments show that our in vivo preparation is amenable to TPLSM: with excitation intensities low enough not to cause photodamage, activity-dependent fluorescence changes of Ca(2+)-sensitive dyes can be detected in small neuronal branches. The performance of two-photon and conventional Ca(2+) imaging carried out consecutively at the same neuron is compared and it is demonstrated that two-photon imaging allows us to detect differences in Ca(2+) dynamics between individual neurites.
Publishing Year
ISSN
PUB-ID

Cite this

Kalb J, Nielsen T, Fricke M, Egelhaaf M, Kurtz R. In vivo two-photon laser-scanning microscopy of Ca 2+ dynamics in visual motion-sensitive neurons. Biochemical and biophysical research communications. 2004;316(2):341-347.
Kalb, J., Nielsen, T., Fricke, M., Egelhaaf, M., & Kurtz, R. (2004). In vivo two-photon laser-scanning microscopy of Ca 2+ dynamics in visual motion-sensitive neurons. Biochemical and biophysical research communications, 316(2), 341-347. doi:10.1016/j.bbrc.2004.02.047
Kalb, J., Nielsen, T., Fricke, M., Egelhaaf, M., and Kurtz, R. (2004). In vivo two-photon laser-scanning microscopy of Ca 2+ dynamics in visual motion-sensitive neurons. Biochemical and biophysical research communications 316, 341-347.
Kalb, J., et al., 2004. In vivo two-photon laser-scanning microscopy of Ca 2+ dynamics in visual motion-sensitive neurons. Biochemical and biophysical research communications, 316(2), p 341-347.
J. Kalb, et al., “In vivo two-photon laser-scanning microscopy of Ca 2+ dynamics in visual motion-sensitive neurons”, Biochemical and biophysical research communications, vol. 316, 2004, pp. 341-347.
Kalb, J., Nielsen, T., Fricke, M., Egelhaaf, M., Kurtz, R.: In vivo two-photon laser-scanning microscopy of Ca 2+ dynamics in visual motion-sensitive neurons. Biochemical and biophysical research communications. 316, 341-347 (2004).
Kalb, Julia, Nielsen, Tim, Fricke, Matthias, Egelhaaf, Martin, and Kurtz, Rafael. “In vivo two-photon laser-scanning microscopy of Ca 2+ dynamics in visual motion-sensitive neurons”. Biochemical and biophysical research communications 316.2 (2004): 341-347.
All files available under the following license(s):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Main File(s)
Access Level
OA Open Access

This data publication is cited in the following publications:
This publication cites the following data publications:

7 Citations in Europe PMC

Data provided by Europe PubMed Central.

Representation of thermal information in the antennal lobe of leaf-cutting ants.
Ruchty M, Helmchen F, Wehner R, Kleineidam CJ., Front Behav Neurosci 4(), 2010
PMID: 21120133
Scattering suppression and confocal detection in multifocal multiphoton microscopy.
Martini J, Andresen V, Anselmetti D., J Biomed Opt 12(3), 2007
PMID: 17614718
Application of multiline two-photon microscopy to functional in vivo imaging.
Kurtz R, Fricke M, Kalb J, Tinnefeld P, Sauer M., J. Neurosci. Methods 151(2), 2006
PMID: 16442636

32 References

Data provided by Europe PubMed Central.

The number and structure of giant vertical cells (VS) in the lobula plate of the blowfly Calliphora erythrocephala
Hengstenberg, J. Comp. Physiol. A 149(), 1982
Highly nonlinear photodamage in two-photon fluorescence microscopy.
Hopt A, Neher E., Biophys. J. 80(4), 2001
PMID: 11259316
High efficiency beam splitter for multifocal multiphoton microscopy.
Nielsen T, Fricke M, Hellweg D, Andresen P., J Microsc 201(Pt 3), 2001
PMID: 11240852
Pulse-length dependence of cellular response to intense near-infrared laser pulses in multiphoton microscopes.
Konig K, Becker TW, Fischer P, Riemann I, Halbhuber KJ., Opt Lett 24(2), 1999
PMID: 18071425

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 15020223
PubMed | Europe PMC

Search this title in

Google Scholar