Supramolecular structure of a new family of circular proteoglycans mediating cell adhesion in sponges

Jarchow J, Fritz J, Anselmetti D, Calabro A, Hascall VC, Gerosa D, Burger MM, Fernandez-Busquets X (2000)
Journal of structural biology 132(2): 95-105.

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Abstract / Bemerkung
Aggregation factors are the molecules responsible for species-specific cell adhesion in sponges. Here, we present the structure of the aggregation factor from the marine sponge Microciona prolifera, which constitutes the first description of a circular proteoglycan. We have analyzed chemically dissociated and enzymatically digested aggregation factor with atomic force microscopy, agarose gel electrophoresis, and Western blots using antibodies against the protein and carbohydrate moieties. Twenty units from each of two N-glycosylated proteins, MAFp3 and MAFp4, form the central ring and radiating arms, respectively, stabilized by a hyaluronidase-sensitive component. MAFp3 carries a 200-kDa glycan involved in homologous self-interactions between aggregation factor molecules, whereas MAFp4 carries a 6-kDa glycan that binds cell surface receptors. A 68-kDa lectin found in cell membranes of several sponge species binds the aggregation factor and its protein-free glycans, as well as chondroitin sulfate and hyaluronan. Here, we show that despite their lack of clear sequence homologies with other known proteoglycan structures, the protein and carbohydrate components of sponge aggregation factors assemble to form a supramolecular complex remarkably similar to classical proteoglycans.
Erscheinungsjahr
Zeitschriftentitel
Journal of structural biology
Band
132
Zeitschriftennummer
2
Seite
95-105
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Jarchow J, Fritz J, Anselmetti D, et al. Supramolecular structure of a new family of circular proteoglycans mediating cell adhesion in sponges. Journal of structural biology. 2000;132(2):95-105.
Jarchow, J., Fritz, J., Anselmetti, D., Calabro, A., Hascall, V. C., Gerosa, D., Burger, M. M., et al. (2000). Supramolecular structure of a new family of circular proteoglycans mediating cell adhesion in sponges. Journal of structural biology, 132(2), 95-105. doi:10.1006/jsbi.2000.4309
Jarchow, J., Fritz, J., Anselmetti, D., Calabro, A., Hascall, V. C., Gerosa, D., Burger, M. M., and Fernandez-Busquets, X. (2000). Supramolecular structure of a new family of circular proteoglycans mediating cell adhesion in sponges. Journal of structural biology 132, 95-105.
Jarchow, J., et al., 2000. Supramolecular structure of a new family of circular proteoglycans mediating cell adhesion in sponges. Journal of structural biology, 132(2), p 95-105.
J. Jarchow, et al., “Supramolecular structure of a new family of circular proteoglycans mediating cell adhesion in sponges”, Journal of structural biology, vol. 132, 2000, pp. 95-105.
Jarchow, J., Fritz, J., Anselmetti, D., Calabro, A., Hascall, V.C., Gerosa, D., Burger, M.M., Fernandez-Busquets, X.: Supramolecular structure of a new family of circular proteoglycans mediating cell adhesion in sponges. Journal of structural biology. 132, 95-105 (2000).
Jarchow, Janina, Fritz, Jürgen, Anselmetti, Dario, Calabro, Anthony, Hascall, Vincent C., Gerosa, Daniela, Burger, Max M., and Fernandez-Busquets, Xavier. “Supramolecular structure of a new family of circular proteoglycans mediating cell adhesion in sponges”. Journal of structural biology 132.2 (2000): 95-105.
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21 Zitationen in Europe PMC

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PMID: 23468137
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PMID: 18843533
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41 References

Daten bereitgestellt von Europe PubMed Central.

Ubiquitin and ubiquitination in cells from the marine sponge Geodia cydonium.
Muller WE, Gamulin V, Rinkevich B, Spreitzer I, Weinblum D, Schroder HC., Biol. Chem. Hoppe-Seyler 375(1), 1994
PMID: 8003257
Species-specific aggregation factor in sponges. V. Influence on programmed syntheses.
Muller WE, Muller I, Zahn RK., Biochim. Biophys. Acta 418(2), 1976
PMID: 55274
Structure of low density heparan sulfate proteoglycan isolated from a mouse tumor basement membrane.
Paulsson M, Yurchenco PD, Ruben GC, Engel J, Timpl R., J. Mol. Biol. 197(2), 1987
PMID: 2960821
Cloning of the polyubiquitin cDNA from the marine sponge Geodia cydonium and its preferential expression during reaggregation of cells.
Pfeifer K, Frank W, Schroder HC, Gamulin V, Rinkevich B, Batel R, Muller IM, Muller WE., J. Cell. Sci. 106 ( Pt 2)(), 1993
PMID: 8282761
The cadherin family
Pigott, 1993

Sambrook, 1989
Induction of ras gene expression by homologous aggregation factor in cells from the sponge Geodia cydonium.
Schroder HC, Kuchino Y, Gramzow M, Kurelec B, Friese U, Uhlenbruck G, Muller WE., J. Biol. Chem. 263(31), 1988
PMID: 2460447
Characterization of a novel pyruvylated carbohydrate unit implicated in the cell aggregation of the marine sponge Microciona prolifera.
Spillmann D, Hard K, Thomas-Oates J, Vliegenthart JF, Misevic G, Burger MM, Finne J., J. Biol. Chem. 268(18), 1993
PMID: 8514776
Characterization of a novel sulfated carbohydrate unit implicated in the carbohydrate-carbohydrate-mediated cell aggregation of the marine sponge Microciona prolifera.
Spillmann D, Thomas-Oates JE, van Kuik JA, Vliegenthart JF, Misevic G, Burger MM, Finne J., J. Biol. Chem. 270(10), 1995
PMID: 7890617
Two component system for surface guided reassociation of animal cells.
Weinbaum G, Burger MM., Nature 244(5417), 1973
PMID: 4621125

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