Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy

Hansmeier N, Bartels FW, Ros R, Anselmetti D, Tauch A, Pühler A, Kalinowski J (2004)
J Biotechnol 112(1-2): 177-193.

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Abstract
The structural S-layer proteins of 28 different Corynebacterium glutamicum isolates have been analyzed systematically. Treatment of whole C. glutamicum cells with detergents resulted in the isolation of S-layer proteins with different apparent molecular masses, ranging in size from 55 to 66 kDa. The S-layer genes analyzed were characterized by coding regions ranging from 1473 to 1533 nucleotides coding for S-layer proteins with a size of 490-510 amino acids. Using PCR techniques, the corresponding S-layer genes of the 28 C. glutamicum isolates were all cloned and sequenced. The deduced amino acid sequences of the S-layer proteins showed identities between 69 and 98% and could be grouped into five phylogenetic classes. Furthermore, sequence analyses indicated that the S-layer proteins of the analyzed C. glutamicum isolates exhibit a mosaic structure of highly conserved and highly variable regions. Several conserved regions were assumed to play a key role in the formation of the C. glutamicum S-layers. Especially the N-terminal signal peptides and the C-terminal anchor sequences of the S-layer proteins showed a nearly perfect amino acid sequence conservation. Analyses by atomic force microscopy revealed a committed hexagonal structure. Morphological diversity of the C. glutamicum S-layers was observed in a class-specific unit cell dimension (ranging from 15.2 to 17.4 nm), which correlates with the sequence similarity-based classification. It could be demonstrated that differences in the primary structure of the S-layer proteins were reflected by the S-layer morphology. (C) 2004 Elsevier B.V. All rights reserved.
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Hansmeier N, Bartels FW, Ros R, et al. Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy. J Biotechnol. 2004;112(1-2):177-193.
Hansmeier, N., Bartels, F. W., Ros, R., Anselmetti, D., Tauch, A., Pühler, A., & Kalinowski, J. (2004). Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy. J Biotechnol, 112(1-2), 177-193.
Hansmeier, N., Bartels, F. W., Ros, R., Anselmetti, D., Tauch, A., Pühler, A., and Kalinowski, J. (2004). Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy. J Biotechnol 112, 177-193.
Hansmeier, N., et al., 2004. Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy. J Biotechnol, 112(1-2), p 177-193.
N. Hansmeier, et al., “Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy”, J Biotechnol, vol. 112, 2004, pp. 177-193.
Hansmeier, N., Bartels, F.W., Ros, R., Anselmetti, D., Tauch, A., Pühler, A., Kalinowski, J.: Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy. J Biotechnol. 112, 177-193 (2004).
Hansmeier, Nicole, Bartels, Frank Wilco, Ros, Robert, Anselmetti, Dario, Tauch, Andreas, Pühler, Alfred, and Kalinowski, Jörn. “Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy”. J Biotechnol 112.1-2 (2004): 177-193.
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