DIRECT CORRELATION OF PARVALBUMIN LEVELS WITH MYOSIN ISOFORMS AND SUCCINATE-DEHYDROGENASE ACTIVITY ON FROZEN-SECTIONS OF RODENT MUSCLE

FUCHTBAUER EM, ROWLERSON AM, GOTZ K, FRIEDRICH G, MABUCHI K, GERGELY J, Jockusch H (1991)
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 39(3): 355-361.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ;
Abstract
Parvalbumin (PV) is a soluble Ca++ binding protein which is particularly concentrated in fast muscles of rodents. We have developed a new protocol to fix frozen sections of muscle by formaldehyde vapor, which enabled us to immunochemically stain serial frozen sections for PV. Fiber types were defined on the basis of myosin ATPase stability, and of isomyosins identified by a variety of antibodies because ATPase stability alone yielded ambiguous results in the mouse. Slow Type I fibers in mouse and rat were devoid of PV and had intermediate to high SDH levels. Fast fiber subtypes IIA, IIB, and IIX-like were defined in the mouse on the basis of the similarity of their myosin heavy chain immunoreactivity to these types in the rat. The soleus muscle was usually PV negative, but a small population of strongly PV-positive IIX-like fibers was present in the mouse. In mouse fast muscle, small diameter IIA fibers were PV negative with high SDH activity. In both mouse and rat, PV reactivities of IIB and IIX fibers were higher than those of IIA and I, whereas SDH levels of IIA, IIX, and I fibers were higher than those of IIB. Thus, PV content correlated with the type of myosin ATPase but not with SDH levels. The method described for immunocytochemistry of PV may be applicable to other highly soluble proteins.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

FUCHTBAUER EM, ROWLERSON AM, GOTZ K, et al. DIRECT CORRELATION OF PARVALBUMIN LEVELS WITH MYOSIN ISOFORMS AND SUCCINATE-DEHYDROGENASE ACTIVITY ON FROZEN-SECTIONS OF RODENT MUSCLE. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. 1991;39(3):355-361.
FUCHTBAUER, E. M., ROWLERSON, A. M., GOTZ, K., FRIEDRICH, G., MABUCHI, K., GERGELY, J., & Jockusch, H. (1991). DIRECT CORRELATION OF PARVALBUMIN LEVELS WITH MYOSIN ISOFORMS AND SUCCINATE-DEHYDROGENASE ACTIVITY ON FROZEN-SECTIONS OF RODENT MUSCLE. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 39(3), 355-361. doi:10.1177/39.3.1825216
FUCHTBAUER, E. M., ROWLERSON, A. M., GOTZ, K., FRIEDRICH, G., MABUCHI, K., GERGELY, J., and Jockusch, H. (1991). DIRECT CORRELATION OF PARVALBUMIN LEVELS WITH MYOSIN ISOFORMS AND SUCCINATE-DEHYDROGENASE ACTIVITY ON FROZEN-SECTIONS OF RODENT MUSCLE. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 39, 355-361.
FUCHTBAUER, E.M., et al., 1991. DIRECT CORRELATION OF PARVALBUMIN LEVELS WITH MYOSIN ISOFORMS AND SUCCINATE-DEHYDROGENASE ACTIVITY ON FROZEN-SECTIONS OF RODENT MUSCLE. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 39(3), p 355-361.
E.M. FUCHTBAUER, et al., “DIRECT CORRELATION OF PARVALBUMIN LEVELS WITH MYOSIN ISOFORMS AND SUCCINATE-DEHYDROGENASE ACTIVITY ON FROZEN-SECTIONS OF RODENT MUSCLE”, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, vol. 39, 1991, pp. 355-361.
FUCHTBAUER, E.M., ROWLERSON, A.M., GOTZ, K., FRIEDRICH, G., MABUCHI, K., GERGELY, J., Jockusch, H.: DIRECT CORRELATION OF PARVALBUMIN LEVELS WITH MYOSIN ISOFORMS AND SUCCINATE-DEHYDROGENASE ACTIVITY ON FROZEN-SECTIONS OF RODENT MUSCLE. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. 39, 355-361 (1991).
FUCHTBAUER, EM, ROWLERSON, AM, GOTZ, K, FRIEDRICH, G, MABUCHI, K, GERGELY, J, and Jockusch, Harald. “DIRECT CORRELATION OF PARVALBUMIN LEVELS WITH MYOSIN ISOFORMS AND SUCCINATE-DEHYDROGENASE ACTIVITY ON FROZEN-SECTIONS OF RODENT MUSCLE”. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 39.3 (1991): 355-361.
This data publication is cited in the following publications:
This publication cites the following data publications:

31 Citations in Europe PMC

Data provided by Europe PubMed Central.

The excitation-contraction coupling mechanism in skeletal muscle.
Calderon JC, Bolanos P, Caputo C., Biophys Rev 6(1), 2014
PMID: 28509964
Rats bred for low aerobic capacity become promptly fatigued and have slow metabolic recovery after stimulated, maximal muscle contractions.
Torvinen S, Silvennoinen M, Piitulainen H, Narvainen J, Tuunanen P, Grohn O, Koch LG, Britton SL, Kainulainen H., PLoS ONE 7(11), 2012
PMID: 23185253
Different regulatory sequences are required for parvalbumin gene expression in skeletal muscles and neuronal cells of transgenic mice.
Hazama M, Watanabe D, Suzuki M, Mizoguchi A, Pastan I, Nakanishi S., Brain Res. Mol. Brain Res. 100(1-2), 2002
PMID: 12008021
Parvalbumin distribution in the musculature of the pharyngo-oesophageal segment.
Imamura R, Yoshida Y, Fukunaga H, Nakashima T, Hirano M., Acta Otolaryngol. 118(6), 1998
PMID: 9870637

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 1825216
PubMed | Europe PMC

Search this title in

Google Scholar