GENETIC-ANALYSIS OF THE PHOSPHINOTHRICIN-TRIPEPTIDE BIOSYNTHETIC-PATHWAY OF STREPTOMYCES-VIRIDOCHROMOGENES TU494

ALIJAH R, DORENDORF J, TALAY S, Pühler A, WOHLLEBEN W (1991)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 34(6): 749-755.

Download
No fulltext has been uploaded. References only!
Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Streptomyces viridochromogenes Tu494 produces the antibiotic phosphinothricyl-alanyl-alanine (Ptt). Ptt-non-producing mutants were isolated following N-methyl-N(t)-nitro-N-nitrosoguanidine (NTG) or UV light treatment of spore suspensions. In co-synthesis and bioconversion experiments the mutational blocks in the biosynthetic pathway were localized. The mutant NTG1 was analysed in detail. This mutant acts as a secretor for all other mutants. From bioconversion experiments with presumptive precursors circumstantial evidence was obtained that NTG1 is mutated in a gene involved in the alanylation of N-acetyl-demethyl-phosphinothricin. Using a cosmid gene library the DNA region complementing the defective gene of mutant NTG1 was isolated on a 4-kb BamHI fragment. Subcloning experiments showed that a 3-kb BglII/BamHI fragment is sufficient for complementation of mutant NTG1.
Publishing Year
ISSN
PUB-ID

Cite this

ALIJAH R, DORENDORF J, TALAY S, Pühler A, WOHLLEBEN W. GENETIC-ANALYSIS OF THE PHOSPHINOTHRICIN-TRIPEPTIDE BIOSYNTHETIC-PATHWAY OF STREPTOMYCES-VIRIDOCHROMOGENES TU494. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1991;34(6):749-755.
ALIJAH, R., DORENDORF, J., TALAY, S., Pühler, A., & WOHLLEBEN, W. (1991). GENETIC-ANALYSIS OF THE PHOSPHINOTHRICIN-TRIPEPTIDE BIOSYNTHETIC-PATHWAY OF STREPTOMYCES-VIRIDOCHROMOGENES TU494. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 34(6), 749-755.
ALIJAH, R., DORENDORF, J., TALAY, S., Pühler, A., and WOHLLEBEN, W. (1991). GENETIC-ANALYSIS OF THE PHOSPHINOTHRICIN-TRIPEPTIDE BIOSYNTHETIC-PATHWAY OF STREPTOMYCES-VIRIDOCHROMOGENES TU494. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 34, 749-755.
ALIJAH, R., et al., 1991. GENETIC-ANALYSIS OF THE PHOSPHINOTHRICIN-TRIPEPTIDE BIOSYNTHETIC-PATHWAY OF STREPTOMYCES-VIRIDOCHROMOGENES TU494. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 34(6), p 749-755.
R. ALIJAH, et al., “GENETIC-ANALYSIS OF THE PHOSPHINOTHRICIN-TRIPEPTIDE BIOSYNTHETIC-PATHWAY OF STREPTOMYCES-VIRIDOCHROMOGENES TU494”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 34, 1991, pp. 749-755.
ALIJAH, R., DORENDORF, J., TALAY, S., Pühler, A., WOHLLEBEN, W.: GENETIC-ANALYSIS OF THE PHOSPHINOTHRICIN-TRIPEPTIDE BIOSYNTHETIC-PATHWAY OF STREPTOMYCES-VIRIDOCHROMOGENES TU494. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 34, 749-755 (1991).
ALIJAH, R, DORENDORF, J, TALAY, S, Pühler, Alfred, and WOHLLEBEN, W. “GENETIC-ANALYSIS OF THE PHOSPHINOTHRICIN-TRIPEPTIDE BIOSYNTHETIC-PATHWAY OF STREPTOMYCES-VIRIDOCHROMOGENES TU494”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 34.6 (1991): 749-755.
This data publication is cited in the following publications:
This publication cites the following data publications:

14 Citations in Europe PMC

Data provided by Europe PubMed Central.

The bifunctional role of aconitase in Streptomyces viridochromogenes Tu494.
Michta E, Schad K, Blin K, Ort-Winklbauer R, Rottig M, Kohlbacher O, Wohlleben W, Schinko E, Mast Y., Environ. Microbiol. 14(12), 2012
PMID: 23116164
Biosynthesis of phosphonic and phosphinic acid natural products.
Metcalf WW, van der Donk WA., Annu. Rev. Biochem. 78(), 2009
PMID: 19489722
Three thioesterases are involved in the biosynthesis of phosphinothricin tripeptide in Streptomyces viridochromogenes Tu494.
Eys S, Schwartz D, Wohlleben W, Schinko E., Antimicrob. Agents Chemother. 52(5), 2008
PMID: 18285472
Biosynthesis of 2-hydroxyethylphosphonate, an unexpected intermediate common to multiple phosphonate biosynthetic pathways.
Shao Z, Blodgett JA, Circello BT, Eliot AC, Woodyer R, Li G, van der Donk WA, Metcalf WW, Zhao H., J. Biol. Chem. 283(34), 2008
PMID: 18544530
Sequencing and analysis of the biosynthetic gene cluster of the lipopeptide antibiotic Friulimicin in Actinoplanes friuliensis.
Muller C, Nolden S, Gebhardt P, Heinzelmann E, Lange C, Puk O, Welzel K, Wohlleben W, Schwartz D., Antimicrob. Agents Chemother. 51(3), 2007
PMID: 17220414
Unusual transformations in the biosynthesis of the antibiotic phosphinothricin tripeptide.
Blodgett JA, Thomas PM, Li G, Velasquez JE, van der Donk WA, Kelleher NL, Metcalf WW., Nat. Chem. Biol. 3(8), 2007
PMID: 17632514
Phosphinothricin tripeptide synthetases in Streptomyces viridochromogenes Tu494.
Schwartz D, Grammel N, Heinzelmann E, Keller U, Wohlleben W., Antimicrob. Agents Chemother. 49(11), 2005
PMID: 16251301
Biosynthetic gene cluster of the herbicide phosphinothricin tripeptide from Streptomyces viridochromogenes Tu494.
Schwartz D, Berger S, Heinzelmann E, Muschko K, Welzel K, Wohlleben W., Appl. Environ. Microbiol. 70(12), 2004
PMID: 15574905
The phosphinomethylmalate isomerase gene pmi, encoding an aconitase-like enzyme, is involved in the synthesis of phosphinothricin tripeptide in Streptomyces viridochromogenes.
Heinzelmann E, Kienzlen G, Kaspar S, Recktenwald J, Wohlleben W, Schwartz D., Appl. Environ. Microbiol. 67(8), 2001
PMID: 11472937
Identification and characterization of phosphinothricin-tripeptide biosynthetic genes in Streptomyces viridochromogenes.
Wohlleben W, Alijah R, Dorendorf J, Hillemann D, Nussbaumer B, Pelzer S., Gene 115(1-2), 1992
PMID: 1612426

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 1367200
PubMed | Europe PMC

Search this title in

Google Scholar