PROPERTIES OF OLIGOMERIC FORMS OF PHOSPHOFRUCTOKINASE FROM CHLORELLA-KESSLERI GROWN UNDER DIFFERENT LIGHT CONDITIONS

Grotjohann N, KOWALLIK W, RUYTERS G (1991)
BOTANICA ACTA 104(1): 77-81.

Journal Article | Published | English

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Abstract
Fast protein liquid chromatography on Superose 6 of crude extracts from Chlorella kessleri, Fott et Novakova, grown autotrophically in blue or in red light yields there different oligomeric forms of phosphofructokinase (PFK, EC 2.7.1.11). Their substrate affinities and responses to homotropic and heterotropic effectors are different. In vitro, the degree of oligomerization of the enzyme can be influenced by specific intermediates or cofactors. Its substrate, MgATP (10 mM/5 mM), and the negative effector, phosphoenolpyruvate (5 mM), both lead to some dissociation, while the second substrate, fructose-6-phosphate (5 mM), and the positive effector, inorganic phosphate (50 mM), have no effect. It is discussed whether formation or dissociation of oligomeric PFK forms in vivo result from alterations in the levels or in the intracellular distribution of effector molecules and whether such processes are involved in the different regulation of cell metabolism in blue or in red light.
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Grotjohann N, KOWALLIK W, RUYTERS G. PROPERTIES OF OLIGOMERIC FORMS OF PHOSPHOFRUCTOKINASE FROM CHLORELLA-KESSLERI GROWN UNDER DIFFERENT LIGHT CONDITIONS. BOTANICA ACTA. 1991;104(1):77-81.
Grotjohann, N., KOWALLIK, W., & RUYTERS, G. (1991). PROPERTIES OF OLIGOMERIC FORMS OF PHOSPHOFRUCTOKINASE FROM CHLORELLA-KESSLERI GROWN UNDER DIFFERENT LIGHT CONDITIONS. BOTANICA ACTA, 104(1), 77-81.
Grotjohann, N., KOWALLIK, W., and RUYTERS, G. (1991). PROPERTIES OF OLIGOMERIC FORMS OF PHOSPHOFRUCTOKINASE FROM CHLORELLA-KESSLERI GROWN UNDER DIFFERENT LIGHT CONDITIONS. BOTANICA ACTA 104, 77-81.
Grotjohann, N., KOWALLIK, W., & RUYTERS, G., 1991. PROPERTIES OF OLIGOMERIC FORMS OF PHOSPHOFRUCTOKINASE FROM CHLORELLA-KESSLERI GROWN UNDER DIFFERENT LIGHT CONDITIONS. BOTANICA ACTA, 104(1), p 77-81.
N. Grotjohann, W. KOWALLIK, and G. RUYTERS, “PROPERTIES OF OLIGOMERIC FORMS OF PHOSPHOFRUCTOKINASE FROM CHLORELLA-KESSLERI GROWN UNDER DIFFERENT LIGHT CONDITIONS”, BOTANICA ACTA, vol. 104, 1991, pp. 77-81.
Grotjohann, N., KOWALLIK, W., RUYTERS, G.: PROPERTIES OF OLIGOMERIC FORMS OF PHOSPHOFRUCTOKINASE FROM CHLORELLA-KESSLERI GROWN UNDER DIFFERENT LIGHT CONDITIONS. BOTANICA ACTA. 104, 77-81 (1991).
Grotjohann, Norbert, KOWALLIK, W, and RUYTERS, G. “PROPERTIES OF OLIGOMERIC FORMS OF PHOSPHOFRUCTOKINASE FROM CHLORELLA-KESSLERI GROWN UNDER DIFFERENT LIGHT CONDITIONS”. BOTANICA ACTA 104.1 (1991): 77-81.
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