Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum

Kalinowski J, Cremer J, Bachmann B, Eggeling L, Sahm H, Pühler A (1991)
MOLECULAR MICROBIOLOGY 5(5): 1197-1204.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ;
Abstract / Bemerkung
The lysC/asd gene cluster of Corynebacterium glutamicum ATCC 13032 was cloned and sequenced. The lysC locus coding for aspartokinase consists of two in-frame overlapping genes, lysC-alpha encoding a protein of 421 amino acids (M(r) 44300) and lysC-beta encoding a protein of 172 amino acids (M(r) 18600). The C. glutamicum aspartokinase was purified and found to contain two proteins of M(r) 47 000 and M(r) 18 000. A C. glutamicum mutant expressing a feedback-resistant aspartokinase was shown to be changed in a single base pair of the lysC-beta gene, leading to an amino acid exchange in the beta-subunit of the aspartokinase. In addition, the identified mutation was found to be responsible for the enhanced expression of the asd gene located downstream of lysC.
Erscheinungsjahr
Zeitschriftentitel
MOLECULAR MICROBIOLOGY
Band
5
Zeitschriftennummer
5
Seite
1197-1204
ISSN
eISSN
PUB-ID

Zitieren

Kalinowski J, Cremer J, Bachmann B, Eggeling L, Sahm H, Pühler A. Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY. 1991;5(5):1197-1204.
Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., & Pühler, A. (1991). Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY, 5(5), 1197-1204. doi:10.1111/j.1365-2958.1991.tb01893.x
Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., and Pühler, A. (1991). Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY 5, 1197-1204.
Kalinowski, J., et al., 1991. Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY, 5(5), p 1197-1204.
J. Kalinowski, et al., “Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum”, MOLECULAR MICROBIOLOGY, vol. 5, 1991, pp. 1197-1204.
Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., Pühler, A.: Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY. 5, 1197-1204 (1991).
Kalinowski, Jörn, Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., and Pühler, Alfred. “Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum”. MOLECULAR MICROBIOLOGY 5.5 (1991): 1197-1204.

67 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Recent advances in the physiology and genetics of amino acid-producing bacteria.
Jetten MS, Sinskey AJ., Crit Rev Biotechnol 15(1), 1995
PMID: 7736600
Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria.
Cirillo JD, Weisbrod TR, Pascopella L, Bloom BR, Jacobs WR., Mol Microbiol 11(4), 1994
PMID: 7910936
Molecular analysis of the aspartate kinase-homoserine dehydrogenase gene from Arabidopsis thaliana.
Ghislain M, Frankard V, Vandenbossche D, Matthews BF, Jacobs M., Plant Mol Biol 24(6), 1994
PMID: 8204822
Metabolic engineering of Corynebacterium glutamicum.
Jetten MS, Follettie MT, Sinskey AJ., Ann N Y Acad Sci 721(), 1994
PMID: 8010662
Molecular aspects of lysine, threonine, and isoleucine biosynthesis in Corynebacterium glutamicum.
Eikmanns BJ, Eggeling L, Sahm H., Antonie Van Leeuwenhoek 64(2), 1993
PMID: 8092856
Gene structure and expression of the Corynebacterium flavum N13 ask-asd operon.
Follettie MT, Peoples OP, Agoropoulou C, Sinskey AJ., J Bacteriol 175(13), 1993
PMID: 8100567
Isolation of a mutant allele that deregulates the threonine biosynthesis in Saccharomyces cerevisiae.
Martin-Rendon E, Farfán MJ, Ramos C, Calderon IL., Curr Genet 24(6), 1993
PMID: 8299165
Functional and structural analyses of threonine dehydratase from Corynebacterium glutamicum.
Möckel B, Eggeling L, Sahm H., J Bacteriol 174(24), 1992
PMID: 1459955
Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake.
Seep-Feldhaus AH, Kalinowski J, Pühler A., Mol Microbiol 5(12), 1991
PMID: 1667221

27 References

Daten bereitgestellt von Europe PubMed Central.

Cloning of a DNA fragment from Corynebacterium glutamicum conferring aminoethyl cysteine resistance and feedback resistance to aspartokinase.
Thierbach G, Kalinowski J, Bachmann B, Puhler A., Appl. Microbiol. Biotechnol. 32(4), 1990
PMID: 1366393

AUTHOR UNKNOWN, 0

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 1956296
PubMed | Europe PMC

Suchen in

Google Scholar