Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum

Kalinowski J, Cremer J, Bachmann B, Eggeling L, Sahm H, Pühler A (1991)
MOLECULAR MICROBIOLOGY 5(5): 1197-1204.

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Abstract
The lysC/asd gene cluster of Corynebacterium glutamicum ATCC 13032 was cloned and sequenced. The lysC locus coding for aspartokinase consists of two in-frame overlapping genes, lysC-alpha encoding a protein of 421 amino acids (M(r) 44300) and lysC-beta encoding a protein of 172 amino acids (M(r) 18600). The C. glutamicum aspartokinase was purified and found to contain two proteins of M(r) 47 000 and M(r) 18 000. A C. glutamicum mutant expressing a feedback-resistant aspartokinase was shown to be changed in a single base pair of the lysC-beta gene, leading to an amino acid exchange in the beta-subunit of the aspartokinase. In addition, the identified mutation was found to be responsible for the enhanced expression of the asd gene located downstream of lysC.
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Kalinowski J, Cremer J, Bachmann B, Eggeling L, Sahm H, Pühler A. Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY. 1991;5(5):1197-1204.
Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., & Pühler, A. (1991). Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY, 5(5), 1197-1204.
Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., and Pühler, A. (1991). Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY 5, 1197-1204.
Kalinowski, J., et al., 1991. Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY, 5(5), p 1197-1204.
J. Kalinowski, et al., “Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum”, MOLECULAR MICROBIOLOGY, vol. 5, 1991, pp. 1197-1204.
Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., Pühler, A.: Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. MOLECULAR MICROBIOLOGY. 5, 1197-1204 (1991).
Kalinowski, Jörn, Cremer, J., Bachmann, B., Eggeling, L., Sahm, H., and Pühler, Alfred. “Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum”. MOLECULAR MICROBIOLOGY 5.5 (1991): 1197-1204.
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27 References

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Cloning of a DNA fragment from Corynebacterium glutamicum conferring aminoethyl cysteine resistance and feedback resistance to aspartokinase.
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