THE RHIZOBIUM-MELILOTI EXOZL EXOB FRAGMENT OF MEGAPLASMID-2 - EXOB FUNCTIONS AS A UDP-GLUCOSE 4-EPIMERASE AND EXOZ SHOWS HOMOLOGY TO NODX OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN TOM

BUENDIA AM, ENENKEL B, KOPLIN R, Niehaus K, Arnold W, Pühler A (1991)
MOLECULAR MICROBIOLOGY 5(6): 1519-1530.

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Abstract
A 2.6 kb ClaI-BamHI DNA fragment of megaplasmid 2 of Rhizobium meliloti 2011 was found to carry genes involved in exopolysaccharide synthesis and infection of alfalfa nodules. The analysis of the nucleotide sequence of this DNA fragment revealed the existence of two open reading frames (ORFs) running in opposite directions. Plasmid integration mutagenesis showed that these ORFs are organized as two monocistronic transcription units. One of the ORFs represents a new exo gene designated exoZ, which is involved in, but not essential for, the production of acidic exopolysaccharide. However, exoZ is not necessary for nodule formation with alfalfa. The ExoZ protein was found to show homology (23.3%) to the NodX protein of the R. leguminosarum biovar viciae strain TOM, known to be essential for nodulating the primitive Afghanistan pea. The second identified ORF corresponds to the exoB locus. The deduced amino acid sequence of the ExoB protein is homologous (39.6%) to that of the Escherichia coli GaIE protein. In R. meliloti, exoB codes for a UDP-glucose 4-epimerase. A deficiency in the activity of this enzyme fully accounts for all the multiple carbohydrate defects that have been observed in exoB mutants.
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BUENDIA AM, ENENKEL B, KOPLIN R, Niehaus K, Arnold W, Pühler A. THE RHIZOBIUM-MELILOTI EXOZL EXOB FRAGMENT OF MEGAPLASMID-2 - EXOB FUNCTIONS AS A UDP-GLUCOSE 4-EPIMERASE AND EXOZ SHOWS HOMOLOGY TO NODX OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN TOM. MOLECULAR MICROBIOLOGY. 1991;5(6):1519-1530.
BUENDIA, A. M., ENENKEL, B., KOPLIN, R., Niehaus, K., Arnold, W., & Pühler, A. (1991). THE RHIZOBIUM-MELILOTI EXOZL EXOB FRAGMENT OF MEGAPLASMID-2 - EXOB FUNCTIONS AS A UDP-GLUCOSE 4-EPIMERASE AND EXOZ SHOWS HOMOLOGY TO NODX OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN TOM. MOLECULAR MICROBIOLOGY, 5(6), 1519-1530.
BUENDIA, A. M., ENENKEL, B., KOPLIN, R., Niehaus, K., Arnold, W., and Pühler, A. (1991). THE RHIZOBIUM-MELILOTI EXOZL EXOB FRAGMENT OF MEGAPLASMID-2 - EXOB FUNCTIONS AS A UDP-GLUCOSE 4-EPIMERASE AND EXOZ SHOWS HOMOLOGY TO NODX OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN TOM. MOLECULAR MICROBIOLOGY 5, 1519-1530.
BUENDIA, A.M., et al., 1991. THE RHIZOBIUM-MELILOTI EXOZL EXOB FRAGMENT OF MEGAPLASMID-2 - EXOB FUNCTIONS AS A UDP-GLUCOSE 4-EPIMERASE AND EXOZ SHOWS HOMOLOGY TO NODX OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN TOM. MOLECULAR MICROBIOLOGY, 5(6), p 1519-1530.
A.M. BUENDIA, et al., “THE RHIZOBIUM-MELILOTI EXOZL EXOB FRAGMENT OF MEGAPLASMID-2 - EXOB FUNCTIONS AS A UDP-GLUCOSE 4-EPIMERASE AND EXOZ SHOWS HOMOLOGY TO NODX OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN TOM”, MOLECULAR MICROBIOLOGY, vol. 5, 1991, pp. 1519-1530.
BUENDIA, A.M., ENENKEL, B., KOPLIN, R., Niehaus, K., Arnold, W., Pühler, A.: THE RHIZOBIUM-MELILOTI EXOZL EXOB FRAGMENT OF MEGAPLASMID-2 - EXOB FUNCTIONS AS A UDP-GLUCOSE 4-EPIMERASE AND EXOZ SHOWS HOMOLOGY TO NODX OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN TOM. MOLECULAR MICROBIOLOGY. 5, 1519-1530 (1991).
BUENDIA, AM, ENENKEL, B, KOPLIN, R, Niehaus, Karsten, Arnold, Walter, and Pühler, Alfred. “THE RHIZOBIUM-MELILOTI EXOZL EXOB FRAGMENT OF MEGAPLASMID-2 - EXOB FUNCTIONS AS A UDP-GLUCOSE 4-EPIMERASE AND EXOZ SHOWS HOMOLOGY TO NODX OF RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN TOM”. MOLECULAR MICROBIOLOGY 5.6 (1991): 1519-1530.
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Different phenotypic classes of Sinorhizobium meliloti mutants defective in synthesis of K antigen.
Campbell GR, Reuhs BL, Walker GC., J. Bacteriol. 180(20), 1998
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Rhizobium extracellular structures in the symbiosis.
Coronado C, Sanchez-Andujar B, Palomares AJ., World J. Microbiol. Biotechnol. 12(2), 1996
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The genetic and biochemical basis for nodulation of legumes by rhizobia.
Pueppke SG., Crit. Rev. Biotechnol. 16(1), 1996
PMID: 8935908

62 References

Data provided by Europe PubMed Central.


Priefer, 1984

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0

Scott, Nucl Acids Res 14(), 1986

Schwedock, Mot Plant-Microbe Interact 2(), 1989

AUTHOR UNKNOWN, 0

Staden, Nucl Acids Res 14(), 1986

Staden, Nucl Acids Res 10(), 1982

Vincent, 1970
A second exopolysaccharide of Rhizobium meliloti strain SU47 that can function in root nodule invasion.
Zhan HJ, Levery SB, Lee CC, Leigh JA., Proc. Natl. Acad. Sci. U.S.A. 86(9), 1989
PMID: 2717610

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