DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS

BRAND T, MILTING H, ZIPPEL M, Jockusch H (1991)
FEBS LETTERS 283(2): 289-290.

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Dissociated cells from neonatal rat atria and ventricles were cultured in monolayers for 3 days. Newly synthesized S-35-methionine labeled myosin light chain isoforms ALC-1 ALC-2 (atrial) and VLC-1, VLC-2 (ventricular) were identified on 2D gels, and their pattern of synthesis was compared to that of myocard fragments immediately after explanatation. ALCs were synthesized in 5- to 10-fold excess over VLCs by atrial cultures, whereas the converse was true for ventricular cultures, with two exceptions: one third of the LC-1 synthesized by ventricular fragments was ALC-1, and dissociated atrial cells synthesized very little LC-2 of either isoform. The former finding corresponds to the relatively high proportion of ALC-1 in neonatal ventricular tissue. We conclude that the regional programme of LC isoform expression is basically retained after tissue explanation and even after dissociation and culturing of cardiac myocytes.
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BRAND T, MILTING H, ZIPPEL M, Jockusch H. DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS. 1991;283(2):289-290.
BRAND, T., MILTING, H., ZIPPEL, M., & Jockusch, H. (1991). DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS, 283(2), 289-290.
BRAND, T., MILTING, H., ZIPPEL, M., and Jockusch, H. (1991). DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS 283, 289-290.
BRAND, T., et al., 1991. DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS, 283(2), p 289-290.
T. BRAND, et al., “DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS”, FEBS LETTERS, vol. 283, 1991, pp. 289-290.
BRAND, T., MILTING, H., ZIPPEL, M., Jockusch, H.: DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS. 283, 289-290 (1991).
BRAND, T, MILTING, H, ZIPPEL, M, and Jockusch, Harald. “DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS”. FEBS LETTERS 283.2 (1991): 289-290.
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PMID: 2044768
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