CHARACTERIZATION OF RECA GENES AND RECA MUTANTS OF RHIZOBIUM-MELILOTI AND RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE

Selbitschka W, Arnold W, PRIEFER UB, ROTTSCHAFER T, SCHMIDT M, SIMON R, Pühler A (1991)
MOLECULAR & GENERAL GENETICS 229(1): 86-95.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ;
Abstract
DNA fragments carrying the recA genes of Rhizobium meliloti and Rhizobium leguminosarum biovar viciae were isolated by complementing a UV-sensitive recA- Escherichia coli strain. Sequence analysis revealed that the coding region of the R. meliloti recA gene consists of 1044 bp coding for 348 amino acids whereas the coding region of the R. leguminosarum bv. viciae recA gene has 1053 bp specifying 351 amino acids. The R. meliloti and R. leguminosarum bv. viciae recA genes show 84.8% homology at the DNA sequence level and of 90.1% at the amino acid sequence level. recA- mutant strains of both Rhizobium species were constructed by inserting a gentamicin resistance cassette into the respective recA gene. The resulting recA mutants exhibited an increased sensitivity to UV irradiation, were impaired in their ability to perform homologous recombination and showed a slightly reduced growth rate when compared with the respective wild-type strains. The Rhizobium recA strains did not have altered symbiotic nitrogen fixation capacity. Therefore, they represent ideal candidates for release experiments with impaired strains.
Publishing Year
ISSN
PUB-ID

Cite this

Selbitschka W, Arnold W, PRIEFER UB, et al. CHARACTERIZATION OF RECA GENES AND RECA MUTANTS OF RHIZOBIUM-MELILOTI AND RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE. MOLECULAR & GENERAL GENETICS. 1991;229(1):86-95.
Selbitschka, W., Arnold, W., PRIEFER, U. B., ROTTSCHAFER, T., SCHMIDT, M., SIMON, R., & Pühler, A. (1991). CHARACTERIZATION OF RECA GENES AND RECA MUTANTS OF RHIZOBIUM-MELILOTI AND RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE. MOLECULAR & GENERAL GENETICS, 229(1), 86-95.
Selbitschka, W., Arnold, W., PRIEFER, U. B., ROTTSCHAFER, T., SCHMIDT, M., SIMON, R., and Pühler, A. (1991). CHARACTERIZATION OF RECA GENES AND RECA MUTANTS OF RHIZOBIUM-MELILOTI AND RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE. MOLECULAR & GENERAL GENETICS 229, 86-95.
Selbitschka, W., et al., 1991. CHARACTERIZATION OF RECA GENES AND RECA MUTANTS OF RHIZOBIUM-MELILOTI AND RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE. MOLECULAR & GENERAL GENETICS, 229(1), p 86-95.
W. Selbitschka, et al., “CHARACTERIZATION OF RECA GENES AND RECA MUTANTS OF RHIZOBIUM-MELILOTI AND RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE”, MOLECULAR & GENERAL GENETICS, vol. 229, 1991, pp. 86-95.
Selbitschka, W., Arnold, W., PRIEFER, U.B., ROTTSCHAFER, T., SCHMIDT, M., SIMON, R., Pühler, A.: CHARACTERIZATION OF RECA GENES AND RECA MUTANTS OF RHIZOBIUM-MELILOTI AND RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE. MOLECULAR & GENERAL GENETICS. 229, 86-95 (1991).
Selbitschka, Werner, Arnold, Walter, PRIEFER, UB, ROTTSCHAFER, T, SCHMIDT, M, SIMON, R, and Pühler, Alfred. “CHARACTERIZATION OF RECA GENES AND RECA MUTANTS OF RHIZOBIUM-MELILOTI AND RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE”. MOLECULAR & GENERAL GENETICS 229.1 (1991): 86-95.
This data publication is cited in the following publications:
This publication cites the following data publications:

14 Citations in Europe PMC

Data provided by Europe PubMed Central.

Identification of genes relevant to symbiosis and competitiveness in Sinorhizobium meliloti using signature-tagged mutants.
Pobigaylo N, Szymczak S, Nattkemper TW, Becker A., Mol. Plant Microbe Interact. 21(2), 2008
PMID: 18184066
Long-term field release of bioluminescent Sinorhizobium meliloti strains to assess the influence of a recA mutation on the strains' survival.
Selbitschka W, Keller M, Miethling-Graff R, Dresing U, Schwieger F, Krahn I, Homann I, Dammann-Kalinowski T, Puhler A, Tebbe CC., Microb. Ecol. 52(3), 2006
PMID: 16924432
Differential gene expression during interactions between Heterobasidion annosum and Physisporinus sanguinolentus.
Iakovlev A, Olson A, Elfstrand M, Stenlid J., FEMS Microbiol. Lett. 241(1), 2004
PMID: 15556713
Reduced survival of a RecA-deficient Sinorhizobium meliloti strain in sterile and non-sterile during heat stress
Dresing U, Hagen M, Selbitschka W, Puhler A, Keller M., FEMS Microbiol. Ecol. 27(4), 1998
PMID: IND21976940
Mutational analysis of the Rhizobium etli recA operator.
Tapias A, Barbe J., J. Bacteriol. 180(23), 1998
PMID: 9829943
Construction and characterization of a Rhizobium leguminosarum biovar viciae strain designed to assess horizontal gene transfer in the environment.
Selbitschka W, Jording D, Nieman S, Schmidt R, Puhler A, Mendum T, Hirsch P., FEMS Microbiol. Lett. 128(3), 1995
PMID: 7781972
A biological containment system for Rhizobium meliloti based on the use of recombination-deficient (recA-) strains.
Selbitschka W, Dresing U, Hagen M, Niemann S, Puhler A., FEMS Microbiol. Ecol. 16(3), 1995
PMID: IND20456986
Interspecies regulation of the recA gene of gram-negative bacteria lacking an E. coli-like SOS operator.
Riera J, Fernandez de Henestrosa AR, Garriga X, Tapias A, Barbe J., Mol. Gen. Genet. 245(4), 1994
PMID: 7808403
Idiographic representation of conserved domain of a class II tRNA synthetase of unknown structure.
Ribas de Pouplana L, Buechter DD, Davis MW, Schimmel P., Protein Sci. 2(12), 1993
PMID: 8298469

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 1896024
PubMed | Europe PMC

Supplements

GenBank
X59957 X59956 S63909 S55532 S55531 S55530 S55529 S55528 S55527 S55291 S55272 S55278

Search this title in

Google Scholar