Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6

Sonnen H, Thierbach G, Kautz S, Kalinowski J, Schneider J, Pühler A, Kutzner HJ (1991)
Gene 107(1): 69-74.

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Abstract
A new plasmid, pGA1, has been isolated from Corynebacterium glutamicum LP-6, and its detailed restriction map has been prepared. The 4.9-kb plasmid has a G + C content of 57%. It replicates in C. glutamicum ATCC13032 and is compatible with the three other plasmids, pCC1, pBL1 and pHM1519, commonly used for vector construction for amino acid-producing corynebacteria. Fusions of pGA1 with different Escherichia coli replicons (transferred from E. coli to Corynebacterium via transformation of spheroplasts or by filter mating experiments with intact cells) are shown to be suitable as shuttle plasmids; some of them are highly stable in C. glutamicum, even when propagated without any selection pressure.
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Sonnen H, Thierbach G, Kautz S, et al. Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6. Gene. 1991;107(1):69-74.
Sonnen, H., Thierbach, G., Kautz, S., Kalinowski, J., Schneider, J., Pühler, A., & Kutzner, H. J. (1991). Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6. Gene, 107(1), 69-74.
Sonnen, H., Thierbach, G., Kautz, S., Kalinowski, J., Schneider, J., Pühler, A., and Kutzner, H. J. (1991). Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6. Gene 107, 69-74.
Sonnen, H., et al., 1991. Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6. Gene, 107(1), p 69-74.
H. Sonnen, et al., “Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6”, Gene, vol. 107, 1991, pp. 69-74.
Sonnen, H., Thierbach, G., Kautz, S., Kalinowski, J., Schneider, J., Pühler, A., Kutzner, H.J.: Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6. Gene. 107, 69-74 (1991).
Sonnen, Hans, Thierbach, Georg, Kautz, Sabine, Kalinowski, Jörn, Schneider, Jörg, Pühler, Alfred, and Kutzner, Hans J. “Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6”. Gene 107.1 (1991): 69-74.
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Seibold G, Auchter M, Berens S, Kalinowski J, Eikmanns BJ., J. Biotechnol. 124(2), 2006
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Hansmeier N, Bartels FW, Ros R, Anselmetti D, Tauch A, Puhler A, Kalinowski J., J. Biotechnol. 112(1-2), 2004
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Plasmids of corynebacteria.
Deb JK, Nath N., FEMS Microbiol. Lett. 175(1), 1999
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Recent advances in the physiology and genetics of amino acid-producing bacteria.
Jetten MS, Sinskey AJ., Crit. Rev. Biotechnol. 15(1), 1995
PMID: 7736600

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