Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake

Seep-Feldhaus AH, Kalinowski J, Pühler A (1991)
MOLECULAR MICROBIOLOGY 5(12): 2995-3005.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
Abstract / Bemerkung
Two Corynebacterium glutamicum mutants defective in lysine uptake were identified by analysing mutants resistant to S-(2-aminoethyl)-cysteine (AEC). A 5.6 kb genomic DNA fragment restoring AEC sensitivity and lysine uptake was isolated. A 4.2 kb subfragment was sequenced and three open reading frames were identified. Subcloning and gene disruption experiments showed that only the first open reading frame, termed lysI, is involved in lysine uptake. LysI consists of 501 amino acids with a M(r) of 53600. The hydrophobicity profile suggests that the lysI gene product is an integral membrane protein with 13 transmembrane segments. The amino acid sequence of lysI displays strong homology to that of the arcD gene product of Pseudomonas aeruginosa, which is proposed to act as an arginine-ornithine antiporter. Investigation of the influence of the lysI gene on lysine secretion suggests the existence of a separate lysine efflux system in C. glutamicum.
Erscheinungsjahr
Zeitschriftentitel
MOLECULAR MICROBIOLOGY
Band
5
Zeitschriftennummer
12
Seite
2995-3005
ISSN
eISSN
PUB-ID

Zitieren

Seep-Feldhaus AH, Kalinowski J, Pühler A. Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake. MOLECULAR MICROBIOLOGY. 1991;5(12):2995-3005.
Seep-Feldhaus, A. H., Kalinowski, J., & Pühler, A. (1991). Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake. MOLECULAR MICROBIOLOGY, 5(12), 2995-3005. doi:10.1111/j.1365-2958.1991.tb01859.x
Seep-Feldhaus, A. H., Kalinowski, J., and Pühler, A. (1991). Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake. MOLECULAR MICROBIOLOGY 5, 2995-3005.
Seep-Feldhaus, A.H., Kalinowski, J., & Pühler, A., 1991. Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake. MOLECULAR MICROBIOLOGY, 5(12), p 2995-3005.
A.H. Seep-Feldhaus, J. Kalinowski, and A. Pühler, “Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake”, MOLECULAR MICROBIOLOGY, vol. 5, 1991, pp. 2995-3005.
Seep-Feldhaus, A.H., Kalinowski, J., Pühler, A.: Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake. MOLECULAR MICROBIOLOGY. 5, 2995-3005 (1991).
Seep-Feldhaus, A. H., Kalinowski, Jörn, and Pühler, Alfred. “Molecular analysis of the Corynebacterium glutamicum lysl gene involved in lysine uptake”. MOLECULAR MICROBIOLOGY 5.12 (1991): 2995-3005.

20 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Anaerobic growth of Corynebacterium glutamicum via mixed-acid fermentation.
Michel A, Koch-Koerfges A, Krumbach K, Brocker M, Bott M., Appl Environ Microbiol 81(21), 2015
PMID: 26276118
Deletion of arcD in Streptococcus pneumoniae D39 impairs its capsule and attenuates virulence.
Gupta R, Yang J, Dong Y, Swiatlo E, Zhang JR, Metzger DW, Bai G., Infect Immun 81(10), 2013
PMID: 23918778
Characterization and molecular mechanism of AroP as an aromatic amino acid and histidine transporter in Corynebacterium glutamicum.
Shang X, Zhang Y, Zhang G, Chai X, Deng A, Liang Y, Wen T., J Bacteriol 195(23), 2013
PMID: 24056108
Identification of the membrane protein SucE and its role in succinate transport in Corynebacterium glutamicum.
Huhn S, Jolkver E, Krämer R, Marin K., Appl Microbiol Biotechnol 89(2), 2011
PMID: 20809072
Biotechnological production of polyamines by bacteria: recent achievements and future perspectives.
Schneider J, Wendisch VF., Appl Microbiol Biotechnol 91(1), 2011
PMID: 21552989
Mapping the membrane proteome of Corynebacterium glutamicum.
Schluesener D, Fischer F, Kruip J, Rögner M, Poetsch A., Proteomics 5(5), 2005
PMID: 15717325
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Krämer R, Linke B, McHardy AC, Meyer F, Möckel B, Pfefferle W, Pühler A, Rey DA, Rückert C, Rupp O, Sahm H, Wendisch VF, Wiegräbe I, Tauch A., J Biotechnol 104(1-3), 2003
PMID: 12948626
Efflux of compatible solutes in Corynebacterium glutamicum mediated by osmoregulated channel activity.
Ruffert S, Lambert C, Peter H, Wendisch VF, Krämer R., Eur J Biochem 247(2), 1997
PMID: 9266699
Transport mutants and transport genes of Corynebacterium glutamicum.
Eggeling L, Krämer R, Vrljic M, Kronemeyer W, Sahm H., Ann N Y Acad Sci 782(), 1996
PMID: 8659896
A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome.
Bathe B, Kalinowski J, Pühler A., Mol Gen Genet 252(3), 1996
PMID: 8842145
Recent advances in the physiology and genetics of amino acid-producing bacteria.
Jetten MS, Sinskey AJ., Crit Rev Biotechnol 15(1), 1995
PMID: 7736600
Structure of the gluABCD cluster encoding the glutamate uptake system of Corynebacterium glutamicum.
Kronemeyer W, Peekhaus N, Krämer R, Sahm H, Eggeling L., J Bacteriol 177(5), 1995
PMID: 7868586
Molecular aspects of lysine, threonine, and isoleucine biosynthesis in Corynebacterium glutamicum.
Eikmanns BJ, Eggeling L, Sahm H., Antonie Van Leeuwenhoek 64(2), 1993
PMID: 8092856

50 References

Daten bereitgestellt von Europe PubMed Central.

Control of transcription termination.
Adhya S, Gottesman M., Annu. Rev. Biochem. 47(), 1978
PMID: 354508
Establishing homologies in protein sequences.
Dayhoff MO, Barker WC, Hunt LT., Meth. Enzymol. 91(), 1983
PMID: 6855599
A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria
Simon, Bio/Technology 1(9), 1983
Cloning Systems in Amino Acid–Producing Corynebacteria
Martín, Bio/Technology 5(2), 1987
The production of -lysine by fermentation
TOSAKA, Trends in Biotechnology 1(3), 1983
Transformation of spheroplasts and protoplasts of Corynebacterium glutamicum
Thierbach, Applied Microbiology and Biotechnology 29(4), 1988
Isoleucine excretion in Corynebacterium glutamicum: evidence for a specific efflux carrier system
Ebbighausen, Applied Microbiology and Biotechnology 31(2), 1989
Evidence for an efflux carrier system involved in the secretion of glutamate by Corynebacterium glutamicum
Hoischen, Archives of Microbiology 151(4), 1989
DNA amplification and an unstable arginine gene in Streptomyces lividans 66.
Altenbuchner J, Cullum J., Mol. Gen. Genet. 195(1-2), 1984
PMID: 6092842
Cloning the dapA dapB cluster of the lysine-secreting bacterium Corynebacterium glutamicum
Cremer, MGG Molecular & General Genetics 220(3), 1990
A rapid boiling method for the preparation of bacterial plasmids.
Holmes DS, Quigley M., Anal. Biochem. 114(1), 1981
PMID: 6269464
Improvements of DNA sequencing gels.
Garoff H, Ansorge W., Anal. Biochem. 115(2), 1981
PMID: 7304971
Cloning of a DNA fragment from Corynebacterium glutamicum conferring aminoethyl cysteine resistance and feedback resistance to aspartokinase.
Thierbach G, Kalinowski J, Bachmann B, Puhler A., Appl. Microbiol. Biotechnol. 32(4), 1990
PMID: 1366393
A simple method for displaying the hydropathic character of a protein.
Kyte J, Doolittle RF., J. Mol. Biol. 157(1), 1982
PMID: 7108955
DNA sequencing with chain-terminating inhibitors.
Sanger F, Nicklen S, Coulson AR., Proc. Natl. Acad. Sci. U.S.A. 74(12), 1977
PMID: 271968
Arginine transport in Streptococcus lactis is catalyzed by a cationic exchanger.
Driessen AJ, Poolman B, Kiewiet R, Konings W., Proc. Natl. Acad. Sci. U.S.A. 84(17), 1987
PMID: 2819865
Rapid and sensitive protein similarity searches.
Lipman DJ, Pearson WR., Science 227(4693), 1985
PMID: 2983426
Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum.
Kalinowski J, Cremer J, Bachmann B, Eggeling L, Sahm H, Puhler A., Mol. Microbiol. 5(5), 1991
PMID: 1956296
Compilation and analysis of Escherichia coli promoter DNA sequences.
Hawley DK, McClure WR., Nucleic Acids Res. 11(8), 1983
PMID: 6344016
The current status and portability of our sequence handling software.
Staden R., Nucleic Acids Res. 14(1), 1986
PMID: 3511446
TAXONOMICAL STUDIES ON GLUTAMIC ACID-PRODUCING BACTERIA
ABE, The Journal of General and Applied Microbiology 13(3), 1967
MICROBIAL PRODUCTION OF L-LYSINE
SANO, The Journal of General and Applied Microbiology 16(5), 1970

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 1667221
PubMed | Europe PMC

Suchen in

Google Scholar