MERCURIAL ACTIVATION OF HUMAN-PMN LEUKOCYTE TYPE-IV PROCOLLAGENASE (GELATINASE)

TRIEBEL S, BLASER J, REINKE H, KNAUPER V, Tschesche H (1992)
FEBS LETTERS 298(2-3): 280-284.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Autoproteolytic activation and processing of human polymorphonuclear leucocyte (PMNL) type IV procollagenase (gelatinase) was initiated by HgCl2 and was investigated by kinetic analysis and N-terminal sequence determination of the reaction products. In the first instance the propeptide domain was lost by subsequent cleavage of the Asp15-Leu16, Glu40-Met41, Leu52-Leu53 and Ala74-Met75 peptide bonds. The PRCGVPD sequence motif (residues Pro78-Asp84), which is conserved in all metalloproteinases and expected to be relevant for latency, remained uncleaved at the activated enzyme. The generated intermediate was further processed by three C-terminal cleavages. The Glu666-Leu667, Ala506-Glu507 and Ala398-Leu399 bonds were hydrolysed sucessively. From the fragmentation products we were able to conclude that three released fragment peptides contained unpaired free cysteine with the residues Cys497, Cys653, Cys683. Cleavage of the first C-terminal peptide bond resulted in the loss of one of the conserved Cys residues of the hemopexin-like domain, whereas the Cys residue of the PRCGVPD motif was retained at the fully active enzyme. The possibility of an entirely different activation mechanism for PMNL type IV procollagenase is discussed.
Publishing Year
ISSN
PUB-ID

Cite this

TRIEBEL S, BLASER J, REINKE H, KNAUPER V, Tschesche H. MERCURIAL ACTIVATION OF HUMAN-PMN LEUKOCYTE TYPE-IV PROCOLLAGENASE (GELATINASE). FEBS LETTERS. 1992;298(2-3):280-284.
TRIEBEL, S., BLASER, J., REINKE, H., KNAUPER, V., & Tschesche, H. (1992). MERCURIAL ACTIVATION OF HUMAN-PMN LEUKOCYTE TYPE-IV PROCOLLAGENASE (GELATINASE). FEBS LETTERS, 298(2-3), 280-284.
TRIEBEL, S., BLASER, J., REINKE, H., KNAUPER, V., and Tschesche, H. (1992). MERCURIAL ACTIVATION OF HUMAN-PMN LEUKOCYTE TYPE-IV PROCOLLAGENASE (GELATINASE). FEBS LETTERS 298, 280-284.
TRIEBEL, S., et al., 1992. MERCURIAL ACTIVATION OF HUMAN-PMN LEUKOCYTE TYPE-IV PROCOLLAGENASE (GELATINASE). FEBS LETTERS, 298(2-3), p 280-284.
S. TRIEBEL, et al., “MERCURIAL ACTIVATION OF HUMAN-PMN LEUKOCYTE TYPE-IV PROCOLLAGENASE (GELATINASE)”, FEBS LETTERS, vol. 298, 1992, pp. 280-284.
TRIEBEL, S., BLASER, J., REINKE, H., KNAUPER, V., Tschesche, H.: MERCURIAL ACTIVATION OF HUMAN-PMN LEUKOCYTE TYPE-IV PROCOLLAGENASE (GELATINASE). FEBS LETTERS. 298, 280-284 (1992).
TRIEBEL, S, BLASER, J, REINKE, H, KNAUPER, V, and Tschesche, Harald. “MERCURIAL ACTIVATION OF HUMAN-PMN LEUKOCYTE TYPE-IV PROCOLLAGENASE (GELATINASE)”. FEBS LETTERS 298.2-3 (1992): 280-284.
This data publication is cited in the following publications:
This publication cites the following data publications:

10 Citations in Europe PMC

Data provided by Europe PubMed Central.

Gelatinase B/MMP-9 in Tumour Pathogenesis and Progression.
Farina AR, Mackay AR., Cancers (Basel) 6(1), 2014
PMID: 24473089
Labeling and enrichment of Arabidopsis thaliana matrix metalloproteases using an active-site directed, marimastat-based photoreactive probe.
Lenger J, Kaschani F, Lenz T, Dalhoff C, Villamor JG, Koster H, Sewald N, van der Hoorn RA., Bioorg. Med. Chem. 20(2), 2012
PMID: 21775155
Regulation of matrix metalloproteinase activity in health and disease.
Hadler-Olsen E, Fadnes B, Sylte I, Uhlin-Hansen L, Winberg JO., FEBS J. 278(1), 2011
PMID: 21087458
Human neutrophil elastase-mediated cleavage sites of MMP-9 and TIMP-1: implications to cystic fibrosis proteolytic dysfunction.
Jackson PL, Xu X, Wilson L, Weathington NM, Clancy JP, Blalock JE, Gaggar A., Mol. Med. 16(5-6), 2010
PMID: 20111696
Induction of matrix metalloproteinase-9 in mice during Toxocara canis larvae migration.
Lai SC, Chen KM, Chen HC, Lee HH., Parasitol. Res. 95(3), 2005
PMID: 15645290
Gelatinase-mediated migration and invasion of cancer cells.
Bjorklund M, Koivunen E., Biochim. Biophys. Acta 1755(1), 2005
PMID: 15907591
The human neutrophil lipocalin supports the allosteric activation of matrix metalloproteinases.
Tschesche H, Zolzer V, Triebel S, Bartsch S., Eur. J. Biochem. 268(7), 2001
PMID: 11277914
The matrix metalloproteinases and their natural inhibitors: prospects for treating degenerative tissue diseases.
Docherty AJ, O'Connell J, Crabbe T, Angal S, Murphy G., Trends Biotechnol. 10(6), 1992
PMID: 1368394
A 25 kDa alpha 2-microglobulin-related protein is a component of the 125 kDa form of human gelatinase.
Triebel S, Blaser J, Reinke H, Tschesche H., FEBS Lett. 314(3), 1992
PMID: 1281792

20 References

Data provided by Europe PubMed Central.

SV40-transformed human lung fibroblasts secrete a 92-kDa type IV collagenase which is identical to that secreted by normal human macrophages.
Wilhelm SM, Collier IE, Marmer BL, Eisen AZ, Grant GA, Goldberg GI., J. Biol. Chem. 264(29), 1989
PMID: 2551898
Characterization of gelatinase from pig polymorphonuclear leucocytes. A metalloproteinase resembling tumour type IV collagenase.
Murphy G, Ward R, Hembry RM, Reynolds JJ, Kuhn K, Tryggvason K., Biochem. J. 258(2), 1989
PMID: 2539808

bläser, eur j biochem (), 1991
The activation of human skin fibroblast procollagenase. Sequence identification of the major conversion products.
Grant GA, Eisen AZ, Marmer BL, Roswit WT, Goldberg GI., J. Biol. Chem. 262(12), 1987
PMID: 3032947

docherty, ann rheumatic dis 49(), 1990

stetler-stevenson, cancer metastasis rev 9(), 1990
Structure-function relationships in the collagenase family member transin.
Sanchez-Lopez R, Nicholson R, Gesnel MC, Matrisian LM, Breathnach R., J. Biol. Chem. 263(24), 1988
PMID: 2841336
Mutational analysis of the transin (rat stromelysin) autoinhibitor region demonstrates a role for residues surrounding the "cysteine switch".
Park AJ, Matrisian LM, Kells AF, Pearson R, Yuan ZY, Navre M., J. Biol. Chem. 266(3), 1991
PMID: 1988438
Synthetic substrates for vertebrate collagenase.
Masui Y, Takemoto T, Sakakibara S, Hori H, Nagai Y., Biochem Med 17(2), 1977
PMID: 193489
Matrix metalloproteinase 2 from human rheumatoid synovial fibroblasts. Purification and activation of the precursor and enzymic properties.
Okada Y, Morodomi T, Enghild JJ, Suzuki K, Yasui A, Nakanishi I, Salvesen G, Nagase H., Eur. J. Biochem. 194(3), 1990
PMID: 2269296
Oxidative autoactivation of latent collagenase by human neutrophils.
Weiss SJ, Peppin G, Ortiz X, Ragsdale C, Test ST., Science 227(4688), 1985
PMID: 2982211
Mechanisms of activation of tissue procollagenase by matrix metalloproteinase 3 (stromelysin).
Suzuki K, Enghild JJ, Morodomi T, Salvesen G, Nagase H., Biochemistry 29(44), 1990
PMID: 2176865
Human fibroblast stromelysin catalytic domain: expression, purification, and characterization of a C-terminally truncated form.
Marcy AI, Eiberger LL, Harrison R, Chan HK, Hutchinson NI, Hagmann WK, Cameron PM, Boulton DA, Hermes JD., Biochemistry 30(26), 1991
PMID: 1647201
Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining
Heukeshoven, Electrophoresis 6(3), 1985
Tissue cooperation in a proteolytic cascade activating human interstitial collagenase.
HE CS, Wilhelm SM, Pentland AP, Marmer BL, Grant GA, Eisen AZ, Goldberg GI., Proc. Natl. Acad. Sci. U.S.A. 86(8), 1989
PMID: 2468156

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 1312026
PubMed | Europe PMC

Search this title in

Google Scholar