TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS

DROGE W, BROER I, Pühler A (1992)
PLANTA 187(1): 142-151.

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L-Phosphinothricin (L-Pt)-resistant plants were constructed by introducing a modified phosphinothricin-N-acetyl-transferase gene (pat) via Agrobacterium-mediated gene transfer into tabacco (Nicotiana tabacum L), and via direct gene transfer into carrot (Daucus carota L). The metabolism of L-Pt was studied in these transgenic, Pt-resistant plants, as well as in the untransformed species. The degradation of L-Pt, C-14-labeled specifically at different C-atoms, was analysed by measuring the release of (CO2)-C-14 and by separating the labeled degradation products on thin-layer-chromatography plates. In untransformed tobacco and carrot plants, L-Pt was deaminated to form its corresponding oxo acid 4-methylphosphinico-2-oxo-butanoic acid (PPO), which subsequently was decarboxylated to form 3-methylphosphinico-propanoic acid (MPP). This compound was stable in plants. A third metabolite remained unidentified. The L-Pt was rapidly N-acetylated in herbicide-resistant tobacco and carrot plants, indicating that the degradation pathway of L-Pt into PPO and MPP was blocked. The N-acetylated product, L-N-acetyl-Pt remained stable with regard to degradation, but was found to exist in a second modified form. In addition, there was a pH-dependent, reversible change in the mobility of L-N-acetyl-Pt thin-layer during chromatography.
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DROGE W, BROER I, Pühler A. TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS. PLANTA. 1992;187(1):142-151.
DROGE, W., BROER, I., & Pühler, A. (1992). TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS. PLANTA, 187(1), 142-151.
DROGE, W., BROER, I., and Pühler, A. (1992). TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS. PLANTA 187, 142-151.
DROGE, W., BROER, I., & Pühler, A., 1992. TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS. PLANTA, 187(1), p 142-151.
W. DROGE, I. BROER, and A. Pühler, “TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS”, PLANTA, vol. 187, 1992, pp. 142-151.
DROGE, W., BROER, I., Pühler, A.: TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS. PLANTA. 187, 142-151 (1992).
DROGE, W, BROER, I, and Pühler, Alfred. “TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS”. PLANTA 187.1 (1992): 142-151.
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