TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS

DROGE W, BROER I, Pühler A (1992)
PLANTA 187(1): 142-151.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ;
Abstract
L-Phosphinothricin (L-Pt)-resistant plants were constructed by introducing a modified phosphinothricin-N-acetyl-transferase gene (pat) via Agrobacterium-mediated gene transfer into tabacco (Nicotiana tabacum L), and via direct gene transfer into carrot (Daucus carota L). The metabolism of L-Pt was studied in these transgenic, Pt-resistant plants, as well as in the untransformed species. The degradation of L-Pt, C-14-labeled specifically at different C-atoms, was analysed by measuring the release of (CO2)-C-14 and by separating the labeled degradation products on thin-layer-chromatography plates. In untransformed tobacco and carrot plants, L-Pt was deaminated to form its corresponding oxo acid 4-methylphosphinico-2-oxo-butanoic acid (PPO), which subsequently was decarboxylated to form 3-methylphosphinico-propanoic acid (MPP). This compound was stable in plants. A third metabolite remained unidentified. The L-Pt was rapidly N-acetylated in herbicide-resistant tobacco and carrot plants, indicating that the degradation pathway of L-Pt into PPO and MPP was blocked. The N-acetylated product, L-N-acetyl-Pt remained stable with regard to degradation, but was found to exist in a second modified form. In addition, there was a pH-dependent, reversible change in the mobility of L-N-acetyl-Pt thin-layer during chromatography.
Publishing Year
ISSN
PUB-ID

Cite this

DROGE W, BROER I, Pühler A. TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS. PLANTA. 1992;187(1):142-151.
DROGE, W., BROER, I., & Pühler, A. (1992). TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS. PLANTA, 187(1), 142-151.
DROGE, W., BROER, I., and Pühler, A. (1992). TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS. PLANTA 187, 142-151.
DROGE, W., BROER, I., & Pühler, A., 1992. TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS. PLANTA, 187(1), p 142-151.
W. DROGE, I. BROER, and A. Pühler, “TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS”, PLANTA, vol. 187, 1992, pp. 142-151.
DROGE, W., BROER, I., Pühler, A.: TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS. PLANTA. 187, 142-151 (1992).
DROGE, W, BROER, I, and Pühler, Alfred. “TRANSGENIC PLANTS CONTAINING THE PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE GENE METABOLIZE THE HERBICIDE L-PHOSPHINOTHRICIN (GLUFOSINATE) DIFFERENTLY FROM UNTRANSFORMED PLANTS”. PLANTA 187.1 (1992): 142-151.
This data publication is cited in the following publications:
This publication cites the following data publications:

15 Citations in Europe PMC

Data provided by Europe PubMed Central.

Characterisation of glufosinate resistance mechanisms in Eleusine indica.
Jalaludin A, Yu Q, Zoellner P, Beffa R, Powles SB., Pest Manag. Sci. 73(6), 2017
PMID: 28094896
Qualitative/quantitative strategy for the determination of glufosinate and metabolites in plants.
Rojano-Delgado AM, Priego-Capote F, De Prado R, Luque de Castro MD., Anal Bioanal Chem 406(2), 2014
PMID: 24253412
D-glufosinate as a male sterility agent for hybrid seed production.
Hawkes T, Pline-Srnic W, Dale R, Friend E, Hollinshead T, Howe P, Thompson P, Viner R, Greenland A., Plant Biotechnol. J. 9(3), 2011
PMID: 20678098
Physiological characteristics of glufosinate resistance in rice
Tsai CJ, Wang CS, Wang CY., Weed Sci. 54(4), 2006
PMID: IND43901156
Physiological Mechanisms of Glyphosate Resistance
Pline-Srnic W., Weed Technol 20(2), 2006
PMID: IND43818559
Herbicidal inhibitors of amino acid biosynthesis and herbicide-tolerant crops.
Tan S, Evans R, Singh B., Amino Acids 30(2), 2006
PMID: 16547651

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 24177979
PubMed | Europe PMC

Search this title in

Google Scholar