THE RHIZOBIUM-MELILOTI PMI GENE ENCODES A NEW TYPE OF PHOSPHOMANNOSE ISOMERASE

SCHMIDT M, Arnold W, NIEMANN A, KLEICKMANN A, Pühler A (1992)
GENE 122(1): 35-43.

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Abstract
Interspecific complementation of a Xanthomonas campestris pv. campestris phosphomannose isomerase (PMI) mutant was used to isolate a cosmid from a genomic library of Rhizobium meliloti 2011 carrying the pmi gene of this strain. Subcloning experiments localized the coding region to a 2.0-kb SalI-ClaI fragment. Nucleotide sequence analysis of this fragment indicated the presence of two open reading frames (ORFs), coding for 18- and 43-kDa polypeptides. The analysis of the gene function by gene disruption experiments showed that ORF2 codes for pmi. A comparison of the deduced amino acid sequence with the corresponding sequences of the Pseudomonas aeruginosa and Escherichia coli PMIs revealed no significant homology, indicating that the isolated gene encodes a new type of PMI. The construction of a pmi-deficient mutant of R. meliloti using the sacB-sacR cassette technique showed that the loss of PMI activity does not affect the symbiotic properties of this strain.
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SCHMIDT M, Arnold W, NIEMANN A, KLEICKMANN A, Pühler A. THE RHIZOBIUM-MELILOTI PMI GENE ENCODES A NEW TYPE OF PHOSPHOMANNOSE ISOMERASE. GENE. 1992;122(1):35-43.
SCHMIDT, M., Arnold, W., NIEMANN, A., KLEICKMANN, A., & Pühler, A. (1992). THE RHIZOBIUM-MELILOTI PMI GENE ENCODES A NEW TYPE OF PHOSPHOMANNOSE ISOMERASE. GENE, 122(1), 35-43.
SCHMIDT, M., Arnold, W., NIEMANN, A., KLEICKMANN, A., and Pühler, A. (1992). THE RHIZOBIUM-MELILOTI PMI GENE ENCODES A NEW TYPE OF PHOSPHOMANNOSE ISOMERASE. GENE 122, 35-43.
SCHMIDT, M., et al., 1992. THE RHIZOBIUM-MELILOTI PMI GENE ENCODES A NEW TYPE OF PHOSPHOMANNOSE ISOMERASE. GENE, 122(1), p 35-43.
M. SCHMIDT, et al., “THE RHIZOBIUM-MELILOTI PMI GENE ENCODES A NEW TYPE OF PHOSPHOMANNOSE ISOMERASE”, GENE, vol. 122, 1992, pp. 35-43.
SCHMIDT, M., Arnold, W., NIEMANN, A., KLEICKMANN, A., Pühler, A.: THE RHIZOBIUM-MELILOTI PMI GENE ENCODES A NEW TYPE OF PHOSPHOMANNOSE ISOMERASE. GENE. 122, 35-43 (1992).
SCHMIDT, M, Arnold, Walter, NIEMANN, A, KLEICKMANN, A, and Pühler, Alfred. “THE RHIZOBIUM-MELILOTI PMI GENE ENCODES A NEW TYPE OF PHOSPHOMANNOSE ISOMERASE”. GENE 122.1 (1992): 35-43.
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